{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T03:42:12Z","timestamp":1790826132977,"version":"4.1.0"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2005,9,1]],"date-time":"2005-09-01T00:00:00Z","timestamp":1125532800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2005,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The location and abundance of<jats:italic>Campylobacter jejuni<\/jats:italic>and<jats:italic>Campylobacter lanienae<\/jats:italic>in the intestines of beef cattle were investigated using real-time quantitative PCR in two studies. In an initial study, digesta and tissue samples were obtained along the digestive tract of two beef steers known to shed<jats:italic>C. jejuni<\/jats:italic>and<jats:italic>C. lanienae<\/jats:italic>(steers A and B). At the time of slaughter, steer B weighed 540 kg, compared to 600 kg for steer A, yet the intestine of steer B (40.5 m) was 36% longer than the intestine of steer A (26.1 m). In total, 323 digesta samples (20-cm intervals) and 998 tissue samples (3.3- to 6.7-cm intervals) were processed.<jats:italic>Campylobacter<\/jats:italic>DNA was detected in the digesta and in association with tissues throughout the small and large intestines of both animals. Although<jats:italic>C. jejuni<\/jats:italic>and<jats:italic>C. lanienae<\/jats:italic>DNA were detected in both animals, only steer A contained substantial quantities of<jats:italic>C. jejuni<\/jats:italic>DNA. In both digesta and tissues of steer A,<jats:italic>C. jejuni<\/jats:italic>was present in the duodenum and jejunum. Considerable quantities of<jats:italic>C. jejuni<\/jats:italic>DNA also were observed in the digesta obtained from the cecum and ascending colon, but minimal DNA was associated with tissues of these regions. In contrast, steer B contained substantial quantities of<jats:italic>C. lanienae<\/jats:italic>DNA, and DNA of this bacterium was limited to the large intestine (i.e., the cecum, proximal ascending colon, descending colon, and rectum); the majority of tissue-associated<jats:italic>C. lanienae<\/jats:italic>DNA was present in the cecum, descending colon, and rectum. In a second study, the location and abundance of<jats:italic>C. jejuni<\/jats:italic>and<jats:italic>C. lanienae<\/jats:italic>DNA were confirmed in the intestines of 20 arbitrarily selected beef cattle. DNA of<jats:italic>C. jejuni<\/jats:italic>and<jats:italic>C. lanienae<\/jats:italic>were detected in the digesta of 57% and 95% of the animals, respectively.<jats:italic>C. jejuni<\/jats:italic>associated with intestinal tissues was most abundant in the duodenum, ileum, and rectum. However, one animal contributed disproportionately to the abundance of<jats:italic>C. jejuni<\/jats:italic>DNA in the ileum and rectum.<jats:italic>C. lanienae<\/jats:italic>was most abundant in the large intestine, and the highest density of DNA of this bacterium was found in the cecum. Therefore,<jats:italic>C. jejuni<\/jats:italic>colonized the proximal small intestine of asymptomatic beef cattle, whereas<jats:italic>C. lanienae<\/jats:italic>primarily resided in the cecum, descending colon, and rectum. This information could be instrumental in developing efficacious strategies to manage the release of these bacteria from the gastrointestinal tracts of cattle.<\/jats:p>","DOI":"10.1128\/aem.71.9.5145-5153.2005","type":"journal-article","created":{"date-parts":[[2005,9,8]],"date-time":"2005-09-08T21:30:13Z","timestamp":1126215013000},"page":"5145-5153","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Colonization of Cattle Intestines by<i>Campylobacter jejuni<\/i>and<i>Campylobacter lanienae<\/i>"],"prefix":"10.1128","volume":"71","author":[{"given":"G. 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