{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:04:22Z","timestamp":1791216262768,"version":"4.1.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2006,2,1]],"date-time":"2006-02-01T00:00:00Z","timestamp":1138752000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Amino acid conversion to aroma compounds by\n            <jats:italic>Lactococcus lactis<\/jats:italic>\n            is limited by the low production of \u03b1-ketoglutarate that is necessary for the first step of conversion. Recently, glutamate dehydrogenase (GDH) activity that catalyzes the reversible glutamate deamination to \u03b1-ketoglutarate was detected in\n            <jats:italic>L. lactis<\/jats:italic>\n            strains isolated from a vegetal source, and the gene responsible for the activity in\n            <jats:italic>L. lactis<\/jats:italic>\n            NCDO1867 was identified and characterized. The gene is located on a 70-kb plasmid also encoding cadmium resistance. In this study,\n            <jats:italic>gdh<\/jats:italic>\n            gene inactivation and overexpression confirmed the direct impact of GDH activity of\n            <jats:italic>L. lactis<\/jats:italic>\n            on amino acid catabolism in a reaction medium at pH 5.5, the pH of cheese. By using cadmium resistance as a selectable marker, the plasmid carrying\n            <jats:italic>gdh<\/jats:italic>\n            was naturally transmitted to another\n            <jats:italic>L. lactis<\/jats:italic>\n            strain by a mating procedure. The transfer conferred to the host strain GDH activity and the ability to catabolize amino acids in the presence of glutamate in the reaction medium. However, the plasmid appeared unstable in a strain also containing the protease lactose plasmid pLP712, indicating an incompatibility between these two plasmids.\n          <\/jats:p>","DOI":"10.1128\/aem.72.2.1402-1409.2006","type":"journal-article","created":{"date-parts":[[2006,2,3]],"date-time":"2006-02-03T21:18:24Z","timestamp":1139001504000},"page":"1402-1409","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Glutamate Dehydrogenase Activity Can Be Transmitted Naturally to\n            <i>Lactococcus lactis<\/i>\n            Strains To Stimulate Amino Acid Conversion to Aroma Compounds"],"prefix":"10.1128","volume":"72","author":[{"given":"Catherine","family":"Tanous","sequence":"first","affiliation":[{"name":"Institut National de la Recherche Agronomique, Unite\u0301 de Biochimie et Structure des Prote\u0301ines, 78352 Jouy en Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilie","family":"Chambellon","sequence":"additional","affiliation":[{"name":"Institut National de la Recherche Agronomique, Unite\u0301 de Biochimie et Structure des Prote\u0301ines, 78352 Jouy en Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Le Bars","sequence":"additional","affiliation":[{"name":"Institut National de la Recherche Agronomique, Unite\u0301 de Biochimie et Structure des Prote\u0301ines, 78352 Jouy en Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilbert","family":"Delespaul","sequence":"additional","affiliation":[{"name":"R &amp; D Fromageries Bel, Vendo\u0302me, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mireille","family":"Yvon","sequence":"additional","affiliation":[{"name":"Institut National de la Recherche Agronomique, Unite\u0301 de Biochimie et Structure des Prote\u0301ines, 78352 Jouy en Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0958-6946(01)00053-X"},{"key":"e_1_3_2_3_2","first-page":"335","volume":"36","year":"1993","unstructured":"Benachenhou-Lahfa, N., P. Forterre, and B. Labedan. 1993. Evolution of glutamate dehydrogenase genes: evidence for two paralogous protein families and unusual branching patterns of the archaebacteria in the universal tree of life. J. Mol. Evol.36:335-346.","journal-title":"J. Mol. Evol."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.11.3628-3635.1993"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/plas.2000.1484"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1631-0691(03)00119-7"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.6.3061-3068.2003"},{"key":"e_1_3_2_9_2","first-page":"367","volume":"238","year":"2004","unstructured":"de la Plaza, M., P. Fernandez de Palencia, C. Pelaez, and T. Requena. 2004. 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