{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T06:20:50Z","timestamp":1790317250750,"version":"4.1.0"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2006,3,1]],"date-time":"2006-03-01T00:00:00Z","timestamp":1141171200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A novel\n                    <jats:italic>Dehalococcoides<\/jats:italic>\n                    isolate capable of metabolic trichloroethene (TCE)-to-ethene reductive dechlorination was obtained from contaminated aquifer material. Growth studies and 16S rRNA gene-targeted analyses suggested culture purity; however, the careful quantitative analysis of\n                    <jats:italic>Dehalococcoides<\/jats:italic>\n                    16S rRNA gene and chloroethene reductive dehalogenase gene (i.e.,\n                    <jats:italic>vcrA<\/jats:italic>\n                    ,\n                    <jats:italic>tceA<\/jats:italic>\n                    , and\n                    <jats:italic>bvcA<\/jats:italic>\n                    ) copy numbers revealed that the culture consisted of multiple, distinct\n                    <jats:italic>Dehalococcoides<\/jats:italic>\n                    organisms. Subsequent transfers, along with quantitative PCR monitoring, yielded isolate GT, possessing only\n                    <jats:italic>vcrA<\/jats:italic>\n                    . These findings suggest that commonly used qualitative 16S rRNA gene-based procedures are insufficient to verify purity of\n                    <jats:italic>Dehalococcoides<\/jats:italic>\n                    cultures. Phylogenetic analysis revealed that strain GT is affiliated with the Pinellas group of the\n                    <jats:italic>Dehalococcoides<\/jats:italic>\n                    cluster and shares 100% 16S rRNA gene sequence identity with two other\n                    <jats:italic>Dehalococcoides<\/jats:italic>\n                    isolates, strain FL2 and strain CBDB1. The new isolate is distinct, as it respires the priority pollutants TCE,\n                    <jats:italic>cis<\/jats:italic>\n                    -1,2-dichloroethene (\n                    <jats:italic>cis<\/jats:italic>\n                    -DCE), 1,1-dichloroethene (1,1-DCE), and vinyl chloride (VC), thereby producing innocuous ethene and inorganic chloride. Strain GT dechlorinated TCE,\n                    <jats:italic>cis<\/jats:italic>\n                    -DCE, 1,1-DCE, and VC to ethene at rates up to 40, 41, 62, and 127 \u03bcmol liter\n                    <jats:sup>\u22121<\/jats:sup>\n                    day\n                    <jats:sup>\u22121<\/jats:sup>\n                    , respectively, but failed to dechlorinate PCE. Hydrogen was the required electron donor, which was depleted to a consumption threshold concentration of 0.76 \u00b1 0.13 nM with VC as the electron acceptor. In contrast to the known TCE dechlorinating isolates, strain GT dechlorinated TCE to ethene with very little formation of chlorinated intermediates, suggesting that this type of organism avoids the commonly observed accumulation of\n                    <jats:italic>cis<\/jats:italic>\n                    -DCE and VC during TCE-to-ethene dechlorination.\n                  <\/jats:p>","DOI":"10.1128\/aem.72.3.1980-1987.2006","type":"journal-article","created":{"date-parts":[[2006,3,3]],"date-time":"2006-03-03T12:47:56Z","timestamp":1141390076000},"page":"1980-1987","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":231,"title":["Quantitative PCR Confirms Purity of Strain GT, a Novel Trichloroethene-to-Ethene-Respiring\n                    <i>Dehalococcoides<\/i>\n                    Isolate"],"prefix":"10.1128","volume":"72","author":[{"given":"Youlboong","family":"Sung","sequence":"first","affiliation":[{"name":"School of Civil and Environmental Engineering"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsti M.","family":"Ritalahti","sequence":"additional","affiliation":[{"name":"School of Civil and Environmental Engineering"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert P.","family":"Apkarian","sequence":"additional","affiliation":[{"name":"Integrated Microscopy and Microanalytical Facility, Department of Chemistry, Emory University, Atlanta, Georgia 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank E.","family":"Lo\u0308ffler","sequence":"additional","affiliation":[{"name":"School of Civil and Environmental Engineering"},{"name":"School of Biology, Georgia Institute of Technology, Atlanta, Georgia 30332-0512"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1026504605443"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/35046063"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.2.953-959.2003"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.9.5538-5545.2004"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/es990638e"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.2.996-1003.2003"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01717"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2005.00830.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.2.485-495.2002"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050577"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.9.5290-5297.2004"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.10.6347-6351.2004"},{"key":"e_1_3_2_14_2","first-page":"1422","volume":"37","year":"2002","unstructured":"Lendvay, J. 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