{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T17:18:20Z","timestamp":1790011100063,"version":"4.0.1"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2006,3,1]],"date-time":"2006-03-01T00:00:00Z","timestamp":1141171200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Phylogenetic analysis of the ketosynthase (KS) gene sequences of marine sponge-derived\n                    <jats:italic>Salinispora<\/jats:italic>\n                    strains of actinobacteria indicated that the polyketide synthase (PKS) gene sequence most closely related to that of\n                    <jats:italic>Salinispora<\/jats:italic>\n                    was the rifamycin B synthase of\n                    <jats:italic>Amycolatopsis mediterranei<\/jats:italic>\n                    . This result was not expected from taxonomic species tree phylogenetics using 16S rRNA sequences. From the PKS sequence data generated from our sponge-derived\n                    <jats:italic>Salinispora<\/jats:italic>\n                    strains, we predicted that such strains might synthesize rifamycin-like compounds. Liquid chromatography-tandem mass spectrometry (LC\/MS\/MS) analysis was applied to one sponge-derived\n                    <jats:italic>Salinispora<\/jats:italic>\n                    strain to test the hypothesis of rifamycin synthesis. The analysis reported here demonstrates that this\n                    <jats:italic>Salinispora<\/jats:italic>\n                    isolate does produce compounds of the rifamycin class, including rifamycin B and rifamycin SV. A rifamycin-specific KS primer set was designed, and that primer set increased the number of rifamycin-positive strains detected by PCR screening relative to the number detectable using a conserved KS-specific set. Thus, the\n                    <jats:italic>Salinispora<\/jats:italic>\n                    group of actinobacteria represents a potential new source of rifamycins outside the genus\n                    <jats:italic>Amycolatopsis<\/jats:italic>\n                    and the first recorded source of rifamycins from marine bacteria.\n                  <\/jats:p>","DOI":"10.1128\/aem.72.3.2118-2125.2006","type":"journal-article","created":{"date-parts":[[2006,3,3]],"date-time":"2006-03-03T12:47:56Z","timestamp":1141390076000},"page":"2118-2125","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":126,"title":["Discovery of a New Source of Rifamycin Antibiotics in Marine Sponge Actinobacteria by Phylogenetic Prediction"],"prefix":"10.1128","volume":"72","author":[{"given":"Tae Kyung","family":"Kim","sequence":"first","affiliation":[{"name":"School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amitha K.","family":"Hewavitharana","sequence":"additional","affiliation":[{"name":"School of Pharmacy, University of Queensland, Brisbane, Queensland 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. Nicholas","family":"Shaw","sequence":"additional","affiliation":[{"name":"School of Pharmacy, University of Queensland, Brisbane, Queensland 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John A.","family":"Fuerst","sequence":"additional","affiliation":[{"name":"School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-5521(98)90141-7"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","unstructured":"Dewick P. M. 2002. Medicinal natural products: a biosynthetic approach. John Wiley Chichester United Kingdom.","DOI":"10.1002\/0470846275"},{"key":"e_1_3_2_5_2","first-page":"164","volume":"5","year":"1989","unstructured":"Felsenstein, J. 1989. PHYLIP\u2014phylogeny inference package (version 3.2). Cladistics5:164-166.","journal-title":"Cladistics"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr960034i"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2005.00785.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2005.00716.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-46-2-383"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.12.7520-7529.2004"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.63625-0"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1128"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.10.5005-5011.2002"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi0489964"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/hlca.19730560717"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1998.tb12861.x"},{"key":"e_1_3_2_17_2","first-page":"146","volume":"14","year":"1959","unstructured":"Sensi, P., P. Margalith, and M. T. Timbal. 1959. Rifomycin, a new antibiotic; preliminary report. Farmaco14:146-147.","journal-title":"Farmaco"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.270.5237.724"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.24.4876"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)93925-1"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.16.9051"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.72.3.2118-2125.2006","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.72.3.2118-2125.2006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,6]],"date-time":"2023-05-06T13:19:57Z","timestamp":1683379197000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.72.3.2118-2125.2006"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,3]]},"references-count":20,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,3]]}},"alternative-id":["10.1128\/AEM.72.3.2118-2125.2006"],"URL":"https:\/\/doi.org\/10.1128\/aem.72.3.2118-2125.2006","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1021511.368998","asserted-by":"object"}]},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,3]]},"assertion":[{"value":"2005-10-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-12-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}