{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T16:23:33Z","timestamp":1790439813222,"version":"4.1.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2006,3,1]],"date-time":"2006-03-01T00:00:00Z","timestamp":1141171200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Unlike\n            <jats:italic>Bacillus subtilis<\/jats:italic>\n            and\n            <jats:italic>Escherichia coli<\/jats:italic>\n            , the gram-positive lactic acid bacterium\n            <jats:italic>Lactococcus lactis<\/jats:italic>\n            does not possess the SecDF protein, a component of the secretion (Sec) machinery involved in late secretion stages and required for the high-capacity protein secretion in\n            <jats:italic>B. subtilis<\/jats:italic>\n            . In this study, we complemented the\n            <jats:italic>L. lactis<\/jats:italic>\n            Sec machinery with SecDF from\n            <jats:italic>B. subtilis<\/jats:italic>\n            and evaluated the effect on the secretion of two forms of staphylococcal nuclease, NucB and NucT, which are efficiently and poorly secreted, respectively. The\n            <jats:italic>B. subtilis<\/jats:italic>\n            SecDF-encoding gene was tested in\n            <jats:italic>L. lactis<\/jats:italic>\n            at different levels. Increased quantities of the precursor and mature forms were observed only at low levels of SecDF and at high NucT production levels. This SecDF secretion enhancement was observed at the optimal growth temperature (30\u00b0C) and was even greater at 15\u00b0C. Furthermore, the introduction of\n            <jats:italic>B. subtilis<\/jats:italic>\n            SecDF into\n            <jats:italic>L. lactis<\/jats:italic>\n            was shown to have a positive effect on a secreted form of\n            <jats:italic>Brucella abortus<\/jats:italic>\n            L7\/L12 antigen.\n          <\/jats:p>","DOI":"10.1128\/aem.72.3.2272-2279.2006","type":"journal-article","created":{"date-parts":[[2006,3,3]],"date-time":"2006-03-03T17:47:56Z","timestamp":1141408076000},"page":"2272-2279","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Complementation of the\n            <i>Lactococcus lactis<\/i>\n            Secretion Machinery with\n            <i>Bacillus subtilis<\/i>\n            SecDF Improves Secretion of Staphylococcal Nuclease"],"prefix":"10.1128","volume":"72","author":[{"given":"S.","family":"Nouaille","sequence":"first","affiliation":[{"name":"Unite\u0301 de Recherches Laitie\u0300res et de Ge\u0301ne\u0301tique Applique\u0301e, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Morello","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Recherches Laitie\u0300res et de Ge\u0301ne\u0301tique Applique\u0301e, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France"},{"name":"GTP-Technology, Prologue Biotech, rue Pierre et Marie Curie, BP 28262, 31682 Labege Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Cortez-Peres","sequence":"additional","affiliation":[{"name":"Unite\u0301 d'Ecologie et Physiologie du Syste\u0300me Digestif, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Le Loir","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie, UMR1253 INRA-Agrocampus, 65 rue de Saint-Brieuc, CS84215 35042 Rennes Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Commissaire","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Recherches Laitie\u0300res et de Ge\u0301ne\u0301tique Applique\u0301e, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. J.","family":"Gratadoux","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Recherches Laitie\u0300res et de Ge\u0301ne\u0301tique Applique\u0301e, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France"},{"name":"Unite\u0301 d'Ecologie et Physiologie du Syste\u0300me Digestif, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Poumerol","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Recherches Laitie\u0300res et de Ge\u0301ne\u0301tique Applique\u0301e, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Gruss","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Recherches Laitie\u0300res et de Ge\u0301ne\u0301tique Applique\u0301e, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Langella","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Recherches Laitie\u0300res et de Ge\u0301ne\u0301tique Applique\u0301e, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France"},{"name":"Unite\u0301 d'Ecologie et Physiologie du Syste\u0300me Digestif, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(03)00475-0"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.33.21217"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.GR-1697R"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.6.1554-1563.1995"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.8.3.545-551.2001"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.14.4391-4399.1993"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.10.3662-3667.1996"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.14.4157-4166.2001"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1992.tb05122.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.5.12.3610-3616.1985"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2003.10.120"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.154.1.1-9.1983"},{"key":"e_1_3_2_14_2","unstructured":"Gibson T. 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