{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:35:29Z","timestamp":1767166529263,"version":"build-2238731810"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1965,1,1]],"date-time":"1965-01-01T00:00:00Z","timestamp":-157766400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Microbiol"],"published-print":{"date-parts":[[1965,1]]},"abstract":"<jats:p>\n                    The effect of nutrients of natural source, such as corn steep liquor, peptone, and yeast extract, on the fermentative production of L-glutamic acid from hydrocarbons by a\n                    <jats:italic>Corynebacterium<\/jats:italic>\n                    was studied. Corn steep liquor and meat extract were found to be remarkably stimulatory to L-glutamic acid production; about 5 g per liter of L-glutamic acid were accumulated in a culture broth containing 3%\n                    <jats:italic>n<\/jats:italic>\n                    -paraffins, 0.01% corn steep liquor, and mineral salts. Among nutritional factors contained in corn steep liquor, biotin had very little effect on the accumulation of L-glutamic acid, but thiamine was highly stimulatory to L-glutamic acid production. The optimal concentration of thiamine for L-glutamic acid production was 3 to 5 \u03bcg per liter, and for cell growth, 50 \u03bcg per liter. L-Glutamic acid was accumulated in negligible quantity when the amount of thiamine in the culture broth was sufficient to support abundant growth of bacterial cells.\n                  <\/jats:p>","DOI":"10.1128\/am.13.1.1-4.1965","type":"journal-article","created":{"date-parts":[[2020,12,5]],"date-time":"2020-12-05T04:57:53Z","timestamp":1607144273000},"page":"1-4","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Effects of Corn Steep Liquor and Thiamine on\n                    <scp>l<\/scp>\n                    -Glutamic Acid Fermentation of Hydrocarbons"],"prefix":"10.1128","volume":"13","author":[{"given":"Joji","family":"Takahashi","sequence":"first","affiliation":[{"name":"Department of Agricultural Chemistry, University of Tokyo, Bunkyo-ku, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaetsu","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Agricultural Chemistry, University of Tokyo, Bunkyo-ku, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukio","family":"Imada","sequence":"additional","affiliation":[{"name":"Department of Agricultural Chemistry, University of Tokyo, Bunkyo-ku, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koichi","family":"Yamada","sequence":"additional","affiliation":[{"name":"Department of Agricultural Chemistry, University of Tokyo, Bunkyo-ku, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/am.13.1.1-4.1965","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/am.13.1.1-4.1965","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T00:51:15Z","timestamp":1645577475000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/am.13.1.1-4.1965"}},"subtitle":["IV. Utilization of Hydrocarbons by Microorganisms"],"short-title":[],"issued":{"date-parts":[[1965,1]]},"references-count":0,"aliases":["10.1128\/aem.13.1.1-4.1965"],"journal-issue":{"issue":"1","published-print":{"date-parts":[[1965,1]]}},"alternative-id":["10.1128\/am.13.1.1-4.1965"],"URL":"https:\/\/doi.org\/10.1128\/am.13.1.1-4.1965","relation":{},"ISSN":["0003-6919"],"issn-type":[{"value":"0003-6919","type":"print"}],"subject":[],"published":{"date-parts":[[1965,1]]},"assertion":[{"value":"1965-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}