{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:28:45Z","timestamp":1767166125645,"version":"build-2238731810"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[1965,7,1]],"date-time":"1965-07-01T00:00:00Z","timestamp":-142128000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Microbiol"],"published-print":{"date-parts":[[1965,7]]},"abstract":"<jats:p>\n                    Polyvalent\n                    <jats:italic>Shigella<\/jats:italic>\n                    conjugates were prepared for\n                    <jats:italic>Shigella<\/jats:italic>\n                    groups A, B, C, and D. After preliminary testing with pure cultures of both homologous and heterologous organisms, these reagents were used in three evaluation studies. Fecal specimens from patients hospitalized with diarrhea, from children involved in an institutional outbreak of dysentery due to\n                    <jats:italic>S. sonnei<\/jats:italic>\n                    , and from patients with diarrhea in Arizona were screened by fluorescent-antibody (FA) tests and were cultured. Specimens were examined at various periods of time after collection and after incubation in broth and saline. Results showed that shigellae were detected most frequently when specimens were cultured immediately after collection. FA tests revealed more positive results when the specimens were incubated in either saline or broth than when they were examined immediately after collection. The\n                    <jats:italic>S. sonnei<\/jats:italic>\n                    conjugate gave the most reliable results of any of the\n                    <jats:italic>Shigella<\/jats:italic>\n                    FA reagents used in these investigations. It proved to be both sensitive and specific.\n                  <\/jats:p>","DOI":"10.1128\/am.13.4.605-613.1965","type":"journal-article","created":{"date-parts":[[2020,12,5]],"date-time":"2020-12-05T04:59:00Z","timestamp":1607144340000},"page":"605-613","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Current Status of Immunofluorescence Techniques for Rapid Detection of Shigellae in Fecal Specimens"],"prefix":"10.1128","volume":"13","author":[{"given":"Berenice M.","family":"Thomason","sequence":"first","affiliation":[{"name":"Communicable Disease Center, U.S. Public Health Service, Atlanta, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Glenda S.","family":"Cowart","sequence":"additional","affiliation":[{"name":"Communicable Disease Center, U.S. Public Health Service, Atlanta, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William B.","family":"Cherry","sequence":"additional","affiliation":[{"name":"Communicable Disease Center, U.S. Public Health Service, Atlanta, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/am.13.4.605-613.1965","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/am.13.4.605-613.1965","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T01:08:05Z","timestamp":1645578485000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/am.13.4.605-613.1965"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1965,7]]},"references-count":0,"aliases":["10.1128\/aem.13.4.605-613.1965"],"journal-issue":{"issue":"4","published-print":{"date-parts":[[1965,7]]}},"alternative-id":["10.1128\/am.13.4.605-613.1965"],"URL":"https:\/\/doi.org\/10.1128\/am.13.4.605-613.1965","relation":{},"ISSN":["0003-6919"],"issn-type":[{"value":"0003-6919","type":"print"}],"subject":[],"published":{"date-parts":[[1965,7]]},"assertion":[{"value":"1965-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}