{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T06:54:49Z","timestamp":1767164089743,"version":"build-2238731810"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1967,3,1]],"date-time":"1967-03-01T00:00:00Z","timestamp":-89596800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Microbiol"],"published-print":{"date-parts":[[1967,3]]},"abstract":"<jats:p>\n                    Soluble antigens from\n                    <jats:italic>Histoplasma capsulatum<\/jats:italic>\n                    in the mycelial and yeast phase were purified by gel filtration, fixed onto paper discs, and employed in an indirect immunofluorescence procedure to detect antibody in sera from individuals infected with\n                    <jats:italic>H. capsulatum<\/jats:italic>\n                    . The elution patterns of crude histoplasmin passed through Sephadex G-200 revealed two minor peaks of protein showing immunofluorescence, complement fixing, and precipitating-antigen activity. A large peak containing the pigment and other low molecular weight materials showed no serological activity. A polysaccharide antigen obtained from fragmented, deproteinized yeast-phase cells was reactive in the fluorescent-antibody test but showed no antigen activity in complement fixation or precipitin tests. Although certain sera from culturally proven cases of blastomycosis, coccidioidomycosis, and cryptococcosis reacted with the purified\n                    <jats:italic>Histoplasma<\/jats:italic>\n                    antigens, preliminary evaluation indicated that the immunofluorescence technique may be of value as a screening procedure for the serodiagnosis of histoplasmosis.\n                  <\/jats:p>","DOI":"10.1128\/am.15.2.350-356.1967","type":"journal-article","created":{"date-parts":[[2020,12,5]],"date-time":"2020-12-05T03:48:12Z","timestamp":1607140092000},"page":"350-356","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Soluble Antigens for Immunofluorescence Detection of\n                    <i>Histoplasma capsulatum<\/i>\n                    Antibodies"],"prefix":"10.1128","volume":"15","author":[{"given":"William A.","family":"Hook","sequence":"first","affiliation":[{"name":"Department of Serology, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D.C."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Earl H.","family":"Fife","sequence":"additional","affiliation":[{"name":"Department of Serology, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D.C."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/am.15.2.350-356.1967","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/am.15.2.350-356.1967","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T02:39:16Z","timestamp":1645583956000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/am.15.2.350-356.1967"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1967,3]]},"references-count":0,"aliases":["10.1128\/aem.15.2.350-356.1967"],"journal-issue":{"issue":"2","published-print":{"date-parts":[[1967,3]]}},"alternative-id":["10.1128\/am.15.2.350-356.1967"],"URL":"https:\/\/doi.org\/10.1128\/am.15.2.350-356.1967","relation":{},"ISSN":["0003-6919"],"issn-type":[{"value":"0003-6919","type":"print"}],"subject":[],"published":{"date-parts":[[1967,3]]},"assertion":[{"value":"1967-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}