{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:51:20Z","timestamp":1767167480318,"version":"build-2238731810"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1968,1,1]],"date-time":"1968-01-01T00:00:00Z","timestamp":-63158400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Microbiol"],"published-print":{"date-parts":[[1968,1]]},"abstract":"<jats:p>\n                    A procedure employing concentration with Sephadex and analysis by gel diffusion (Ouchterlony) was used to detect the toxins of\n                    <jats:italic>Clostridium botulinum<\/jats:italic>\n                    in foods. Botulinal toxins with toxic levels of 370 to 557 mouse LD\n                    <jats:sub>50<\/jats:sub>\n                    per milliliter were detected in the food samples. Test results were verified by use of the mouse protection test. Approximately 24 hr were required to complete the entire procedure.\n                  <\/jats:p>","DOI":"10.1128\/am.16.1.21-24.1968","type":"journal-article","created":{"date-parts":[[2020,12,5]],"date-time":"2020-12-05T03:49:53Z","timestamp":1607140193000},"page":"21-24","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Detection of Botulinal Toxins by Immunodiffusion"],"prefix":"10.1128","volume":"16","author":[{"given":"Barry L.","family":"Vermilyea","sequence":"first","affiliation":[{"name":"Department of Dairy and Food Industry, Iowa State University, Ames, Iowa 50010"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Homer W.","family":"Walker","sequence":"additional","affiliation":[{"name":"Department of Dairy and Food Industry, Iowa State University, Ames, Iowa 50010"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John C.","family":"Ayres","sequence":"additional","affiliation":[{"name":"Department of Dairy and Food Industry, Iowa State University, Ames, Iowa 50010"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/am.16.1.21-24.1968","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/am.16.1.21-24.1968","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T03:38:59Z","timestamp":1645587539000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/am.16.1.21-24.1968"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1968,1]]},"references-count":0,"aliases":["10.1128\/aem.16.1.21-24.1968"],"journal-issue":{"issue":"1","published-print":{"date-parts":[[1968,1]]}},"alternative-id":["10.1128\/am.16.1.21-24.1968"],"URL":"https:\/\/doi.org\/10.1128\/am.16.1.21-24.1968","relation":{},"ISSN":["0003-6919"],"issn-type":[{"value":"0003-6919","type":"print"}],"subject":[],"published":{"date-parts":[[1968,1]]},"assertion":[{"value":"1968-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}