{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:36:06Z","timestamp":1767166566951,"version":"build-2238731810"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1969,3,1]],"date-time":"1969-03-01T00:00:00Z","timestamp":-26438400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Microbiol"],"published-print":{"date-parts":[[1969,3]]},"abstract":"\n Studies were conducted on the interaction of various parameters which affect the storage stability and growth potential of liquid cultures of\n Pasteurella tularensis<\/jats:italic>\n live vaccine strain (LVS) and Rift Valley fever virus Van Wyk strain (RVFV). Storage variables studied with LVS included four storage temperatures (4, -20, -65, -175 C), single and multiple freeze-thaw cycles, two freezing and two thawing rates (slow and fast), various inoculum levels (1, 3, 5, and 10%) for the determination of growth potential, and the retention of immunizing potential (mice and guinea pig) after storage. Neither the freezing rate nor the number of freeze-thaw cycles seriously affected the growth of LVS after storage at -175C; however, the slow rate of thaw proved deleterious as were all temperatures of storage except -175 C after 1 year of storage, as shown by both criteria of evaluation. RVFV produced in two combinations of cell lines and media (LM cell line-199 peptone medium and LDR cell line-Eagle's minimum essential medium) was stored at three serum levels (10, 20, 40%), three\n p<\/jats:italic>\n H values (6.2., 7.0, 7.8), and three temperatures (-20, -65, -175 C). These studies indicated: (i) virus produced in the LDR cell line and Eagle's medium was more stable than that produced in the LM cell line and 199 peptone medium for either short- or long-term storage; (ii) serum levels did not affect stability; and (iii) low\n p<\/jats:italic>\n H resulted in losses during long-term storage under all conditions tested. Thus, cryogenic storage is advantageous for stock culture maintenance of bacteria and viruses and for other similar applications.\n <\/jats:p>","DOI":"10.1128\/am.17.3.427-434.1969","type":"journal-article","created":{"date-parts":[[2020,12,5]],"date-time":"2020-12-05T03:02:03Z","timestamp":1607137323000},"page":"427-434","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Interacting Factors That Influence Long-term Storage of Live\n Pasteurella tularensis<\/i>\n Vaccine and Rift Valley Fever Virus"],"prefix":"10.1128","volume":"17","author":[{"given":"Frederick","family":"Klein","sequence":"first","affiliation":[{"name":"Department of the Army, Fort Detrick, Frederick, Maryland 21701"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerry S.","family":"Walker","sequence":"additional","affiliation":[{"name":"Department of the Army, Fort Detrick, Frederick, Maryland 21701"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bill G.","family":"Mahlandt","sequence":"additional","affiliation":[{"name":"Department of the Army, Fort Detrick, Frederick, Maryland 21701"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard C.","family":"Carter","sequence":"additional","affiliation":[{"name":"Department of the Army, Fort Detrick, Frederick, Maryland 21701"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael D.","family":"Orlando","sequence":"additional","affiliation":[{"name":"Department of the Army, Fort Detrick, Frederick, Maryland 21701"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francis J.","family":"Weirether","sequence":"additional","affiliation":[{"name":"Department of the Army, Fort Detrick, Frederick, Maryland 21701"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralph E.","family":"Lincoln","sequence":"additional","affiliation":[{"name":"Department of the Army, Fort Detrick, Frederick, Maryland 21701"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/am.17.3.427-434.1969","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/am.17.3.427-434.1969","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T04:08:22Z","timestamp":1645589302000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/am.17.3.427-434.1969"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1969,3]]},"references-count":0,"aliases":["10.1128\/aem.17.3.427-434.1969"],"journal-issue":{"issue":"3","published-print":{"date-parts":[[1969,3]]}},"alternative-id":["10.1128\/am.17.3.427-434.1969"],"URL":"https:\/\/doi.org\/10.1128\/am.17.3.427-434.1969","relation":{},"ISSN":["0003-6919"],"issn-type":[{"value":"0003-6919","type":"print"}],"subject":[],"published":{"date-parts":[[1969,3]]},"assertion":[{"value":"1969-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}