{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:53:29Z","timestamp":1767167609070,"version":"build-2238731810"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1970,9,1]],"date-time":"1970-09-01T00:00:00Z","timestamp":20995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Microbiol"],"published-print":{"date-parts":[[1970,9]]},"abstract":"<jats:p>Previous studies have demonstrated that L-forms of bacteria may play a role in persistent, chronic, or recurrent urinary-tract infections. A 2-year program was initiated to determine the feasibility of culturing for L-forms on a routine basis, and to determine the effectiveness of such a program. In relation to the total number of specimens, few L-forms were actually isolated. In comparison with the amount of equipment and technician time required, the return was negligible; only 0.5% of all urine specimens were positive for L-forms. An increase to only 1.2% was noted when culturing for L-forms was limited to patients with a diagnosis of bacteriuria or pyelonephritis. It is recommended that this technique be reserved for those patients with a long history of recurrent urinary-tract infections, after other attempts to cure the patient have met with failure.<\/jats:p>","DOI":"10.1128\/am.20.3.323-327.1970","type":"journal-article","created":{"date-parts":[[2020,12,5]],"date-time":"2020-12-05T04:29:25Z","timestamp":1607142565000},"page":"323-327","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Isolation of L-Forms in a Clinical Microbiology Laboratory"],"prefix":"10.1128","volume":"20","author":[{"given":"John A.","family":"Swierczewski","sequence":"first","affiliation":[{"name":"Department of Microbiology, St. Mary Mercy Hospital, Gary, Indiana 46402"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Perla","family":"Reyes","sequence":"additional","affiliation":[{"name":"Department of Microbiology, St. Mary Mercy Hospital, Gary, Indiana 46402"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/am.20.3.323-327.1970","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/am.20.3.323-327.1970","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T05:58:05Z","timestamp":1645595885000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/am.20.3.323-327.1970"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1970,9]]},"references-count":0,"aliases":["10.1128\/aem.20.3.323-327.1970"],"journal-issue":{"issue":"3","published-print":{"date-parts":[[1970,9]]}},"alternative-id":["10.1128\/am.20.3.323-327.1970"],"URL":"https:\/\/doi.org\/10.1128\/am.20.3.323-327.1970","relation":{},"ISSN":["0003-6919"],"issn-type":[{"value":"0003-6919","type":"print"}],"subject":[],"published":{"date-parts":[[1970,9]]},"assertion":[{"value":"1970-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}