{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:36:09Z","timestamp":1767166569975,"version":"build-2238731810"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[1970,11,1]],"date-time":"1970-11-01T00:00:00Z","timestamp":26265600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Microbiol"],"published-print":{"date-parts":[[1970,11]]},"abstract":"<jats:p>\n                    A practical solvent system for the detection of microorganisms in topical ointments has been developed. The method involves dissolving 0.5 g of topical ointment in 50 ml of a solvent mixture (92 parts isopropyl myristate, 6 parts carbon disulfide, and 2 parts xylene) and filtering it through a 0.45-\u03bcm membrane filter. Residual solvent is then washed from the filter pad with 200 ml of sterile 0.5% Brain Heart Infusion broth containing 0.1% Tween 80. The filter pad is then removed and placed on a petri plate containing Trypticase Soy Agar medium. The petri plates thus prepared are then incubated at 37 C for 7 days, and the colonies produced are then counted. The toxicity of the solvent mixture was determined against\n                    <jats:italic>Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella newington<\/jats:italic>\n                    , and spores of\n                    <jats:italic>Bacillus subtilis<\/jats:italic>\n                    and was found to be less toxic than the heat-sterilized isopropyl myristate and comparable to the filter-sterilized isopropyl myristate.\n                  <\/jats:p>","DOI":"10.1128\/am.20.5.802-804.1970","type":"journal-article","created":{"date-parts":[[2020,12,5]],"date-time":"2020-12-05T03:43:46Z","timestamp":1607139826000},"page":"802-804","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Microcount Method for Petrolatum-Based Topical Ointments Containing Waxes"],"prefix":"10.1128","volume":"20","author":[{"given":"Kiyoshi","family":"Tsuji","sequence":"first","affiliation":[{"name":"Control Analytical Research and Development, The Upjohn Company, Kalamazoo, Michigan 49001"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John H.","family":"Robertson","sequence":"additional","affiliation":[{"name":"Control Analytical Research and Development, The Upjohn Company, Kalamazoo, Michigan 49001"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/am.20.5.802-804.1970","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/am.20.5.802-804.1970","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T06:27:47Z","timestamp":1645597667000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/am.20.5.802-804.1970"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1970,11]]},"references-count":0,"aliases":["10.1128\/aem.20.5.802-804.1970"],"journal-issue":{"issue":"5","published-print":{"date-parts":[[1970,11]]}},"alternative-id":["10.1128\/am.20.5.802-804.1970"],"URL":"https:\/\/doi.org\/10.1128\/am.20.5.802-804.1970","relation":{},"ISSN":["0003-6919"],"issn-type":[{"value":"0003-6919","type":"print"}],"subject":[],"published":{"date-parts":[[1970,11]]},"assertion":[{"value":"1970-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}