{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:46:30Z","timestamp":1767167190392,"version":"build-2238731810"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[1972,5,1]],"date-time":"1972-05-01T00:00:00Z","timestamp":73526400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Microbiol"],"published-print":{"date-parts":[[1972,5]]},"abstract":"<jats:p>\n                    A method for the preparation of purified suspensions of\n                    <jats:italic>Coxiella burneti<\/jats:italic>\n                    by Genetron extraction followed by continuous-flow density gradient ultracentrifugation is described. Both phases of the Henzerling strain of\n                    <jats:italic>C. burneti<\/jats:italic>\n                    were found in a zone between 53 and 65% (w\/w) sucrose. Based on chemical assays, the Genetron zonal rickettsial suspensions were found to be as pure as the rickettsial suspensions which were prepared by the ether extraction method currently in use for producing Q fever vaccines for human use.\n                  <\/jats:p>","DOI":"10.1128\/am.23.5.841-845.1972","type":"journal-article","created":{"date-parts":[[2020,12,5]],"date-time":"2020-12-05T04:22:25Z","timestamp":1607142145000},"page":"841-845","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Preparation of Purified Suspensions of\n                    <i>Coxiella burneti<\/i>\n                    by Genetron Extraction Followed by Continuous-Flow Ultracentrifugation"],"prefix":"10.1128","volume":"23","author":[{"given":"Doria R.","family":"Dubois","sequence":"first","affiliation":[{"name":"Department of Biologics Research, Walter Reed Army Institute of Research, Washington, D.C. 20012"},{"name":"Department of Biology, Catholic University, Washington, D.C. 20017"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ernest C.","family":"Cutchins","sequence":"additional","affiliation":[{"name":"Department of Biologics Research, Walter Reed Army Institute of Research, Washington, D.C. 20012"},{"name":"Department of Biology, Catholic University, Washington, D.C. 20017"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanford","family":"Berman","sequence":"additional","affiliation":[{"name":"Department of Biologics Research, Walter Reed Army Institute of Research, Washington, D.C. 20012"},{"name":"Department of Biology, Catholic University, Washington, D.C. 20017"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph P.","family":"Lowenthal","sequence":"additional","affiliation":[{"name":"Department of Biologics Research, Walter Reed Army Institute of Research, Washington, D.C. 20012"},{"name":"Department of Biology, Catholic University, Washington, D.C. 20017"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert L.","family":"Timchak","sequence":"additional","affiliation":[{"name":"Department of Biologics Research, Walter Reed Army Institute of Research, Washington, D.C. 20012"},{"name":"Department of Biology, Catholic University, Washington, D.C. 20017"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/am.23.5.841-845.1972","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/am.23.5.841-845.1972","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T08:15:39Z","timestamp":1645604139000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/am.23.5.841-845.1972"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,5]]},"references-count":0,"aliases":["10.1128\/aem.23.5.841-845.1972"],"journal-issue":{"issue":"5","published-print":{"date-parts":[[1972,5]]}},"alternative-id":["10.1128\/am.23.5.841-845.1972"],"URL":"https:\/\/doi.org\/10.1128\/am.23.5.841-845.1972","relation":{},"ISSN":["0003-6919"],"issn-type":[{"value":"0003-6919","type":"print"}],"subject":[],"published":{"date-parts":[[1972,5]]},"assertion":[{"value":"1972-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}