{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T01:38:39Z","timestamp":1773279519734,"version":"3.50.1"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[1973,5,1]],"date-time":"1973-05-01T00:00:00Z","timestamp":105062400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Microbiol"],"published-print":{"date-parts":[[1973,5]]},"abstract":"<jats:p>\n            Incorporation of neomycin to the culture medium was found to be effective in inhibiting\n            <jats:italic>Escherichia coli<\/jats:italic>\n            contaminants without interfering with the growth of serotype\n            <jats:italic>L. autumnalis<\/jats:italic>\n            . The growth of 12 other\n            <jats:italic>Leptospira<\/jats:italic>\n            serotypes was unaffected by the addition of 300 \u03bcg of neomycin per ml to Ellinghausen medium or 5 \u03bcg\/ml to Fletcher medium. Neomycin-containing medium was found to be of value in the isolation of leptospiras from cultures of blood from infected laboratory animals. A higher percentage of isolates was obtained in swine kidneys from an abattoir in medium containing neomycin than resulted from the same medium without antibiotic or with 5-fluorouracil. Contaminated leptospiral cultures growing in media with 5-fluorouracil were purified by subculturing into neomycin-containing media.\n          <\/jats:p>","DOI":"10.1128\/am.25.5.781-786.1973","type":"journal-article","created":{"date-parts":[[2020,12,5]],"date-time":"2020-12-05T09:25:12Z","timestamp":1607160312000},"page":"781-786","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Selective Isolation of Leptospiras from Contaminated Material by Incorporation of Neomycin to Culture Media"],"prefix":"10.1128","volume":"25","author":[{"given":"Donald M.","family":"Myers","sequence":"first","affiliation":[{"name":"Pan American Zoonoses Center, Pan American Health Organization, Ramos Mejia, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V\u00edctor M.","family":"Varela-D\u00edaz","sequence":"additional","affiliation":[{"name":"Pan American Zoonoses Center, Pan American Health Organization, Ramos Mejia, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/am.25.5.781-786.1973","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/am.25.5.781-786.1973","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T13:53:21Z","timestamp":1645624401000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/am.25.5.781-786.1973"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,5]]},"references-count":0,"aliases":["10.1128\/aem.25.5.781-786.1973"],"journal-issue":{"issue":"5","published-print":{"date-parts":[[1973,5]]}},"alternative-id":["10.1128\/am.25.5.781-786.1973"],"URL":"https:\/\/doi.org\/10.1128\/am.25.5.781-786.1973","relation":{},"ISSN":["0003-6919"],"issn-type":[{"value":"0003-6919","type":"print"}],"subject":[],"published":{"date-parts":[[1973,5]]},"assertion":[{"value":"1973-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}