{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:31:22Z","timestamp":1767166282098,"version":"build-2238731810"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1973,7,1]],"date-time":"1973-07-01T00:00:00Z","timestamp":110332800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Microbiol"],"published-print":{"date-parts":[[1973,7]]},"abstract":"<jats:p>\n                    The exposure of exponentially growing\n                    <jats:italic>Pseudomonas fluorescens<\/jats:italic>\n                    P7 cells to heating at 36 C for 2 h in a defined medium, followed by cooling to 25 C and further incubation at this, the optimal growth temperature, resulted in the apparent death of approximately 99% of the cells, as determined by their inability to form colonies on Trypticase soy agar. Continued incubation at 25 C resulted in an extremely rapid increase in the Trypticase soy agar count, demonstrating that the phenomenon observed was not death but rather injury. Presumptive evidence of heat-stimulated ribonucleic acid (RNA) degradation and membrane damage was provided by the observed loss of 260-nm absorbing materials. Confirmation of RNA degradation was obtained by colorimetric analysis. Ribosomal RNA from normal and injured cells, which was electrophoretically separated on polyacrylamide gels, revealed that the 23\n                    <jats:italic>S<\/jats:italic>\n                    and 16\n                    <jats:italic>S<\/jats:italic>\n                    species were only partially destroyed. Inhibitor studies demonstrated, however, that RNA synthesis was necessary for recovery. The unusual accumulation of 17\n                    <jats:italic>S<\/jats:italic>\n                    RNA during recovery pointed to the presence of a heat-induced lesion in the RNA maturation process. A thermally induced membrane lesion is also discussed.\n                  <\/jats:p>","DOI":"10.1128\/am.26.1.78-85.1973","type":"journal-article","created":{"date-parts":[[2020,12,5]],"date-time":"2020-12-05T04:15:32Z","timestamp":1607141732000},"page":"78-85","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Characterization of Mild Thermal Stress in\n                    <i>Pseudomonas fluorescens<\/i>\n                    and Its Repair"],"prefix":"10.1128","volume":"26","author":[{"given":"Rodney J. H.","family":"Gray","sequence":"first","affiliation":[{"name":"Department of Food Science, University of Illinois at Urbana-Champaign, Urbana, Illinois"},{"name":"Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lloyd D.","family":"Witter","sequence":"additional","affiliation":[{"name":"Department of Food Science, University of Illinois at Urbana-Champaign, Urbana, Illinois"},{"name":"Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z. John","family":"Ordal","sequence":"additional","affiliation":[{"name":"Department of Food Science, University of Illinois at Urbana-Champaign, Urbana, Illinois"},{"name":"Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/am.26.1.78-85.1973","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/am.26.1.78-85.1973","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T08:44:31Z","timestamp":1645605871000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/am.26.1.78-85.1973"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,7]]},"references-count":0,"aliases":["10.1128\/aem.26.1.78-85.1973"],"journal-issue":{"issue":"1","published-print":{"date-parts":[[1973,7]]}},"alternative-id":["10.1128\/am.26.1.78-85.1973"],"URL":"https:\/\/doi.org\/10.1128\/am.26.1.78-85.1973","relation":{},"ISSN":["0003-6919"],"issn-type":[{"value":"0003-6919","type":"print"}],"subject":[],"published":{"date-parts":[[1973,7]]},"assertion":[{"value":"1973-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}