{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:04:51Z","timestamp":1767164691161,"version":"build-2238731810"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1975,8,1]],"date-time":"1975-08-01T00:00:00Z","timestamp":176083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Microbiol"],"published-print":{"date-parts":[[1975,8]]},"abstract":"<jats:p>\n                    Cells of\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    ML30 in a mineral salts medium were exposed to dichlorodifluoromethane (f-12), cyclopropane, halothane, or Ethrane at concentrations of 1.25, 0.2, 0.04, and 0.008\u00d7 saturation for times up to 1,200 min, and at temperatures in the range of 2 to 37 C. When any of these anesthetics were applied for 300 min at 1.25\u00d7 saturation, a substantial decrease in number of survivors occurred. Halothane was most bactericidal, cyclopropane and Ethrane were moderately bactericidal, and f-12 was least bactericidal. At saturation values of less than 1.0, none of the four anesthetics had an appreciable effect on viability of\n                    <jats:italic>E. coli<\/jats:italic>\n                    . Greatest increases in cell permeability occurred when anesthetics were used at saturation values of 1.25, and permeability changes generally decreased as the concentrations of the chemicals were reduced. In many instances, anesthetics in the vapor state caused significant increases in cell permeability but little or no loss of viability. This indicated that a close relationship did not exist between loss of viability and increased permeability. All four anesthetics caused\n                    <jats:italic>E. coli<\/jats:italic>\n                    to lose substantial and similar amounts of compounds absorbing at 260 nm. Release of compounds absorbing at 260 nm generally increased as the saturation value of a given chemical was increased. Halothane, Ethrane, and cyclopropane but not f-12 caused lysis of\n                    <jats:italic>E. coli<\/jats:italic>\n                    ML30. Considering all results,\n                    <jats:italic>E. coli<\/jats:italic>\n                    ML30 was damaged more by halothane or cyclopropane than by f-12 or Ethrane. When f-12 was applied at a saturation value of 1.25, the bactericidal effect on\n                    <jats:italic>E. coli<\/jats:italic>\n                    was much greater at 37 or 22 C than at 12 or 2 C.\n                  <\/jats:p>","DOI":"10.1128\/am.30.2.178-185.1975","type":"journal-article","created":{"date-parts":[[2020,12,5]],"date-time":"2020-12-05T03:25:57Z","timestamp":1607138757000},"page":"178-185","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Comparative Effects of Anesthetics on the Viability and Integrity of\n                    <i>Escherichia coli<\/i>\n                    ML30"],"prefix":"10.1128","volume":"30","author":[{"given":"B. A.","family":"Prior","sequence":"first","affiliation":[{"name":"Department of Food Science, University of Wisconsin-Madison, Madison, Wisconsin 53706"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.","family":"Fennema","sequence":"additional","affiliation":[{"name":"Department of Food Science, University of Wisconsin-Madison, Madison, Wisconsin 53706"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. H.","family":"Marth","sequence":"additional","affiliation":[{"name":"Department of Food Science, University of Wisconsin-Madison, Madison, Wisconsin 53706"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/am.30.2.178-185.1975","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/am.30.2.178-185.1975","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T09:43:02Z","timestamp":1645609382000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/am.30.2.178-185.1975"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1975,8]]},"references-count":0,"aliases":["10.1128\/aem.30.2.178-185.1975"],"journal-issue":{"issue":"2","published-print":{"date-parts":[[1975,8]]}},"alternative-id":["10.1128\/am.30.2.178-185.1975"],"URL":"https:\/\/doi.org\/10.1128\/am.30.2.178-185.1975","relation":{},"ISSN":["0003-6919"],"issn-type":[{"value":"0003-6919","type":"print"}],"subject":[],"published":{"date-parts":[[1975,8]]},"assertion":[{"value":"1975-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}