{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:47:40Z","timestamp":1767167260956,"version":"build-2238731810"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1975,9,1]],"date-time":"1975-09-01T00:00:00Z","timestamp":178761600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Microbiol"],"published-print":{"date-parts":[[1975,9]]},"abstract":"<jats:p>\n                    Baby hamster kidney (BHK-21) cells were adapted to grow in shaker culture using Waymouth medium 752\/1 containing 20 mM\n                    <jats:italic>N<\/jats:italic>\n                    -2-hydroxyethyl-piperazine-N\u2032-2\u2032-ethanesulfonic acid buffer and supplemented with 2.5% (vol\/vol) calf serum, 0.002% (wt\/vol) sodium oleate, and 0.2% fatty acid-free bovine serum albumin (WO\n                    <jats:sub>2.5<\/jats:sub>\n                    ). Infectivity of Japanese encephalitis virus grown in the cells adapted to WO\n                    <jats:sub>2.5<\/jats:sub>\n                    approached 2 \ufffd 10\n                    <jats:sup>8<\/jats:sup>\n                    plaque-forming units per ml. The culture volume of infected cells was reduced fivefold 12 h after infection. This step resulted in a 10-fold increase in infectivity over that obtained from infected cultures not subjected to volume reduction.\n                  <\/jats:p>","DOI":"10.1128\/am.30.3.433-438.1975","type":"journal-article","created":{"date-parts":[[2020,12,5]],"date-time":"2020-12-05T03:28:00Z","timestamp":1607138880000},"page":"433-438","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Adaptation of BHK-21 Cells to Growth in Shaker Culture and Subsequent Challenge by Japanese Encephalitis Virus"],"prefix":"10.1128","volume":"30","author":[{"given":"Louis E.","family":"Guskey","sequence":"first","affiliation":[{"name":"The Hormel Institute, University of Minnesota, Austin, Minnesota 55912"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Howard M.","family":"Jenkin","sequence":"additional","affiliation":[{"name":"The Hormel Institute, University of Minnesota, Austin, Minnesota 55912"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/am.30.3.433-438.1975","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/am.30.3.433-438.1975","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T09:45:49Z","timestamp":1645609549000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/am.30.3.433-438.1975"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1975,9]]},"references-count":0,"aliases":["10.1128\/aem.30.3.433-438.1975"],"journal-issue":{"issue":"3","published-print":{"date-parts":[[1975,9]]}},"alternative-id":["10.1128\/am.30.3.433-438.1975"],"URL":"https:\/\/doi.org\/10.1128\/am.30.3.433-438.1975","relation":{},"ISSN":["0003-6919"],"issn-type":[{"value":"0003-6919","type":"print"}],"subject":[],"published":{"date-parts":[[1975,9]]},"assertion":[{"value":"1975-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}