{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:27:20Z","timestamp":1785407240103,"version":"3.56.0"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["ASM Case Rep"],"published-print":{"date-parts":[[2025,11,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:sec>\n                      <jats:title>Background<\/jats:title>\n                      <jats:p>\n                        Prompt identification of clinically relevant\n                        <jats:italic toggle=\"yes\">Bordetella<\/jats:italic>\n                        (e.g.,\n                        <jats:italic toggle=\"yes\">B. pertussis<\/jats:italic>\n                        and\n                        <jats:italic toggle=\"yes\">B. parapertussis<\/jats:italic>\n                        ) is critical for both treatment and isolation decisions to mitigate transmission among close contacts. Due to the organisms\u2019 fastidious growth requirements, nucleic acid amplification tests (NAATs) have become a mainstay for identification and differentiation of\n                        <jats:italic toggle=\"yes\">B. pertussis<\/jats:italic>\n                        and\n                        <jats:italic toggle=\"yes\">B. parapertussis<\/jats:italic>\n                        . For NAATs detecting these closely related species, the nucleic acid target dictates assay sensitivity and specificity and varies between commercially available platforms.\n                      <\/jats:p>\n                    <\/jats:sec>\n                    <jats:sec>\n                      <jats:title>Case Summary<\/jats:title>\n                      <jats:p>\n                        This case report describes a child\u2019s course of cough and post-tussive emesis following bone marrow transplant for an underlying immunodeficiency. While an initial BioFire Respiratory Panel 2.1 (BioFire Diagnostics, LLC, Salt Lake City, UT, USA) detected\n                        <jats:italic toggle=\"yes\">B. pertussis<\/jats:italic>\n                        DNA, a subsequent maxillary sinus culture grew\n                        <jats:italic toggle=\"yes\">B. bronchiseptica<\/jats:italic>\n                        , identified by MALDI-TOF MS and whole-genome sequencing. Furthermore, on two subsequent occasions, nasopharyngeal specimens were positive for\n                        <jats:italic toggle=\"yes\">B. pertussis<\/jats:italic>\n                        on the BioFire RP2.1; however, they were negative for\n                        <jats:italic toggle=\"yes\">B. pertussis<\/jats:italic>\n                        on the DiaSorin Molecular Simplexa Bordetella Direct (DiaSorin Molecular LLC, Cypress, CA, USA). These seemingly disparate results can be explained by molecular target differences on the respective platforms and a rare instance of cross-reactivity with the genetic target for\n                        <jats:italic toggle=\"yes\">B. pertussis<\/jats:italic>\n                        (\n                        <jats:italic toggle=\"yes\">ptxP<\/jats:italic>\n                        ) in an isolate of\n                        <jats:italic toggle=\"yes\">B. bronchiseptica<\/jats:italic>\n                        .\n                      <\/jats:p>\n                    <\/jats:sec>\n                    <jats:sec>\n                      <jats:title>Conclusion<\/jats:title>\n                      <jats:p>\n                        This case, where the presence of\n                        <jats:italic toggle=\"yes\">ptxP<\/jats:italic>\n                        was confirmed by whole genome sequencing in an isolate of\n                        <jats:italic toggle=\"yes\">B. bronchiseptica<\/jats:italic>\n                        , highlights a caveat to the molecular diagnosis of pertussis and emphasizes the importance of understanding gene targets to interpret results when false positive detections are suspected.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/asmcr.00084-24","type":"journal-article","created":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T13:00:12Z","timestamp":1757422812000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Molecular methods to diagnose pertussis: a case of confusion with\n                    <i>Bordetella bronchiseptica<\/i>"],"prefix":"10.1128","volume":"1","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1945-8006","authenticated-orcid":false,"given":"Christopher M.","family":"Zarbock","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03x3g5467","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pathology and Immunology, Division of Laboratory and Genomic Medicine, Washington University School of Medicine","place":["St. Louis, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3079-5575","authenticated-orcid":false,"given":"Esse M.","family":"Evbuomwan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03x3g5467","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pathology and Immunology, Division of Laboratory and Genomic Medicine, Washington University School of Medicine","place":["St. Louis, USA"]},{"id":[{"id":"https:\/\/ror.org\/03x3g5467","id-type":"ROR","asserted-by":"publisher"}],"name":"The Edison Family Center for Genome Sciences and Systems Biology, Washington University School of Medicine","place":["St. Louis, USA"]},{"id":[{"id":"https:\/\/ror.org\/01yc7t268","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biomedical Engineering, Washington University in St. Louis","place":["St. Louis, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Shenep","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03x3g5467","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pediatric Infectious Diseases, Washington University School of Medicine","place":["St. Louis, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0455-8370","authenticated-orcid":true,"given":"Gautam","family":"Dantas","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03x3g5467","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pathology and Immunology, Division of Laboratory and Genomic Medicine, Washington University School of Medicine","place":["St. Louis, USA"]},{"id":[{"id":"https:\/\/ror.org\/03x3g5467","id-type":"ROR","asserted-by":"publisher"}],"name":"The Edison Family Center for Genome Sciences and Systems Biology, Washington University School of Medicine","place":["St. Louis, USA"]},{"id":[{"id":"https:\/\/ror.org\/01yc7t268","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biomedical Engineering, Washington University in St. Louis","place":["St. Louis, USA"]},{"id":[{"id":"https:\/\/ror.org\/03x3g5467","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pediatric Infectious Diseases, Washington University School of Medicine","place":["St. Louis, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3139-3153","authenticated-orcid":true,"given":"Rebekah E.","family":"Dumm","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03x3g5467","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pathology and Immunology, Division of Laboratory and Genomic Medicine, Washington University School of Medicine","place":["St. Louis, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.2741\/mattoo"},{"key":"e_1_3_3_3_2","volume-title":"Bordetella and related genera","author":"Kilgore PE","year":"2023","unstructured":"Kilgore PE, Coenye T. 2023. Bordetella and related genera. 13th ed. American Society of Microbiology.","edition":"13"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.2.326-382.2005"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-012-1718-3"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.00164-22"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00783-24"},{"key":"e_1_3_3_8_2","unstructured":"BIOM\u00c9RIEUX. 2023. BIOFIRE respiratory panel 2.1 (RP2.1) package insert. Salt Lake City (UT) BIOFIRE Diagnostics LLC"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005595"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000685"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07641-9"},{"key":"e_1_3_3_12_2","unstructured":"R K. 2018. Pheatmap: pretty heatmaps. R package version 1.0.12. https:\/\/cran.r-project.org\/web\/packages\/pheatmap\/pheatmap.pdf."},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0906412106"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr039"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.1.55-60.2000"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1227"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-13-545"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj014"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1923"},{"key":"e_1_3_3_20_2","unstructured":"DiaSorin. 2018. Simplexa Bordetella direct package insert. Cypress California U.S.A DiaSorin Molecular LLC. https:\/\/www.accessdata.fda.gov\/cdrh_docs\/pdf17\/K173498.pdf."},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jemermed.2012.09.037"}],"container-title":["ASM Case Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/asmcr.00084-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/asmcr.00084-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T13:01:59Z","timestamp":1762261319000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/asmcr.00084-24"}},"subtitle":[],"editor":[{"given":"Nicholas M.","family":"Moore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,11,4]]},"references-count":20,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,11,4]]}},"alternative-id":["10.1128\/asmcr.00084-24"],"URL":"https:\/\/doi.org\/10.1128\/asmcr.00084-24","relation":{},"ISSN":["2996-2684"],"issn-type":[{"value":"2996-2684","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,4]]},"assertion":[{"value":"2025-05-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00084-24"}}