{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T11:58:02Z","timestamp":1780055882242,"version":"3.54.0"},"reference-count":15,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T00:00:00Z","timestamp":1746489600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T00:00:00Z","timestamp":1746489600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["ASM Case Rep"],"published-print":{"date-parts":[[2025,5,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:sec>\n              <jats:title>Background<\/jats:title>\n              <jats:p>Myiases are fly larvae parasitic infections involving host tissues. In humans, myiases generally occur in people with reduced self-care capacity or living in poor hygienic conditions, with wounds serving as an entry point for flies. In addition to direct larvae damage, myiases constitute a risk factor for secondary bacterial infections.<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n              <jats:title>Case Summary<\/jats:title>\n              <jats:p>\n                An 81-year-old patient with multiple comorbidities and living in poor hygienic conditions accessed the emergency room of a secondary care hospital in the Varese area following deterioration in his clinical condition and presence of maggots on his right leg. Blood cultures grew gram-negative bacilli, which could not be identified using standard procedures: biochemical tests yielded\n                <jats:italic toggle=\"yes\">Pasteurella canis<\/jats:italic>\n                as a result with 95% probability, whereas matrix-assisted laser desorption ionization time-of-flight mass spectrometry could not find the spectrum in its database. The species\n                <jats:italic toggle=\"yes\">Ignatzschineria larvae<\/jats:italic>\n                was determined by 16S rRNA sequencing, and its significance was interpreted upon consultation between microbiologists and clinicians.\n              <\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n              <jats:title>Conclusion<\/jats:title>\n              <jats:p>\n                This case underscores the role of collaboration between clinical microbiology laboratories and clinicians in diagnosing and managing uncommon infections. The identification of\n                <jats:italic toggle=\"yes\">I. larvae<\/jats:italic>\n                was obtained through 16S rRNA sequencing, highlighting how sequencing-based approaches are becoming increasingly implemented as diagnostic tools when traditional methods fail in identifying rare microorganisms.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/asmcr.00113-24","type":"journal-article","created":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T09:16:22Z","timestamp":1742894182000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Maggot-associated\n            <i>Ignatzschineria larvae<\/i>\n            bacteremia: a case report"],"prefix":"10.1128","volume":"1","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-5562-5140","authenticated-orcid":false,"given":"Paolo","family":"Gigante","sequence":"first","affiliation":[{"name":"Laboratory of 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Varese","place":["Varese, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigina","family":"Guasti","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery, University of Insubria","place":["Varese, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Novazzi","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Microbiology and Virology, ASST Sette Laghi, University Hospital of Varese","place":["Varese, Italy"]},{"name":"Department of Medicine and Technological Innovation, University of Insubria","place":["Varese, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8484-3205","authenticated-orcid":true,"given":"Mattia","family":"Carbotti","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Microbiology and Virology, ASST Sette Laghi, University Hospital of Varese","place":["Varese, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianluca","family":"Cassani","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Microbiology and Virology, ASST Sette Laghi, University Hospital of Varese","place":["Varese, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domenico","family":"Caleca","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Microbiology and Virology, ASST Sette Laghi, University Hospital of Varese","place":["Varese, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riccardo","family":"Capuano","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Microbiology and Virology, ASST Sette Laghi, University Hospital of Varese","place":["Varese, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9e","family":"Pasciuta","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Microbiology and Virology, ASST 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