{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:17:35Z","timestamp":1785435455894,"version":"3.56.0"},"reference-count":19,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T00:00:00Z","timestamp":1767657600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T00:00:00Z","timestamp":1767657600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["ASM Case Rep"],"published-print":{"date-parts":[[2026,1,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Metagenomic next-generation sequencing (mNGS) in plasma, cerebrospinal fluid (CSF), and bronchoalveolar lavage fluid is a relatively new technology that offers a means to potentially provide a diagnosis in cases where infection is suspected, but conventional diagnostic testing has not revealed a pathogen. There have been many publications of individual cases and overall appraisals of its utility in detecting bacteria, fungi, and DNA viruses associated with otherwise undiagnosed systemic infections. A recent article by Phillips et al. published in\n                      <jats:italic toggle=\"yes\">ASM Case Reports<\/jats:italic>\n                      (2:e00053-25, 2025,\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/doi.org\/10.1128\/asmcr.00053-25\" xlink:type=\"simple\">https:\/\/doi.org\/10.1128\/asmcr.00053-25<\/jats:ext-link>\n                      ) presents a case of meningitis in an immunosuppressed child that was ultimately determined to be caused by\n                      <jats:italic toggle=\"yes\">Trichosporon inkin<\/jats:italic>\n                      using mNGS. Elevated \u00df\u22121,3-D-glucan (BDG) levels in CSF and serum projected a diagnosis of fungal meningitis. Bacterial, fungal, and mycobacterial cultures were negative. Positive lateral flow cryptococcal antigen titers in serum and CSF complicated the anticipated diagnosis since\n                      <jats:italic toggle=\"yes\">Cryptococcus<\/jats:italic>\n                      spp. are thought to not have sufficient cell wall BDG to produce positive test results. Given the ultimate diagnosis of\n                      <jats:italic toggle=\"yes\">T. inkin<\/jats:italic>\n                      meningitis and the known cross-reactivity with\n                      <jats:italic toggle=\"yes\">Trichosporon asahii<\/jats:italic>\n                      per package insert, the unexpected cryptococcal antigen results raised the possibility of additional cross-reactivity. The authors uncovered this possibility by testing three known clinical isolates of\n                      <jats:italic toggle=\"yes\">T. inkin<\/jats:italic>\n                      which generated positive results. This case adds to the growing literature that highlights the utility of mNGS in providing a diagnosis in otherwise unresolved cases and shows that mNGS can be further instructive in elucidating limitations in commonly used diagnostic tests.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/asmcr.00140-25","type":"journal-article","created":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T16:17:18Z","timestamp":1762273038000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Metagenomic next-generation sequencing reveals cross-reactivity of lateral flow cryptococcal antigen assay with\n                    <i>Trichosporon inkin<\/i>"],"prefix":"10.1128","volume":"2","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7055-942X","authenticated-orcid":true,"given":"Eileen M.","family":"Burd","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02gars961","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pathology and Laboratory Medicine, Emory University School of Medicine","place":["Atlanta, USA"]},{"id":[{"id":"https:\/\/ror.org\/02gars961","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine","place":["Atlanta, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/asmcr.00053-25"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa218"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa220"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa041"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinmicnews.2021.04.003"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.01855-22"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics15161991"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.238170.118"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1803396"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1186424"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-024-03275-1"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40291-024-00727-9"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000211779"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02505-24"},{"key":"e_1_3_1_16_2","unstructured":"CrAg LFA Cryptococcal Antigen package insert. Document #:PIS-00107 Rev date 2024-09-19 Rev 3. 2024. Norman Oklahoma USA IMMY Inc."},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2014.12.017"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myac020"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.1.126-130.1995"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00003-11"}],"container-title":["ASM Case Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/asmcr.00140-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/asmcr.00140-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T13:43:21Z","timestamp":1767966201000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/asmcr.00140-25"}},"subtitle":[],"editor":[{"given":"Ritu","family":"Banerjee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1,6]]},"references-count":19,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1,6]]}},"alternative-id":["10.1128\/asmcr.00140-25"],"URL":"https:\/\/doi.org\/10.1128\/asmcr.00140-25","relation":{},"ISSN":["2996-2684"],"issn-type":[{"value":"2996-2684","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,6]]},"assertion":[{"value":"2025-11-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00140-25"}}