{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:23:53Z","timestamp":1785414233198,"version":"3.56.0"},"reference-count":13,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T00:00:00Z","timestamp":1767657600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T00:00:00Z","timestamp":1767657600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["ASM Case Rep"],"published-print":{"date-parts":[[2026,1,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:sec>\n                      <jats:title>Background<\/jats:title>\n                      <jats:p>\n                        Rapidly growing non-tuberculous mycobacteria (NTM) are increasingly recognized as causative agents in healthcare-associated infections.\n                        <jats:italic toggle=\"yes\">Mycobacterium wolinskyi<\/jats:italic>\n                        , first described in 1999, has been associated with post-traumatic and post-operative wound infections.\n                      <\/jats:p>\n                    <\/jats:sec>\n                    <jats:sec>\n                      <jats:title>Case Summary<\/jats:title>\n                      <jats:p>\n                        We report a case of\n                        <jats:italic toggle=\"yes\">M. wolinskyi<\/jats:italic>\n                        pacemaker pocket infection and lead endocarditis in a 73-year-old man, 3 weeks after implantable cardioverter-defibrillator (ICD) implantation. Initial culture from a superficial pocket swab yielded slow-growing, white colonies on chocolate agar, and the strain was identified by MALDI-TOF as\n                        <jats:italic toggle=\"yes\">M. wolinskyi<\/jats:italic>\n                        . However, the 16S rRNA,\n                        <jats:italic toggle=\"yes\">hsp65<\/jats:italic>\n                        ,\n                        <jats:italic toggle=\"yes\">rpoB,<\/jats:italic>\n                        and\n                        <jats:italic toggle=\"yes\">recA<\/jats:italic>\n                        sequences were identical to those of the unofficially described\n                        <jats:italic toggle=\"yes\">M. jacuzzi<\/jats:italic>\n                        species but different from the corresponding sequences of the\n                        <jats:italic toggle=\"yes\">M. wolinskyi<\/jats:italic>\n                        ATCC 700010 reference strain, which showed an average nucleotide identity (ANI) of 97.49% upon whole genome comparison. Therapy consisted of ICD removal, surgical debridement of the pocket, and prolonged combination therapy with oral moxifloxacin and co-trimoxazole, with favorable clinical outcome.\n                      <\/jats:p>\n                    <\/jats:sec>\n                    <jats:sec>\n                      <jats:title>Conclusion<\/jats:title>\n                      <jats:p>\n                        This case demonstrates that our strain of\n                        <jats:italic toggle=\"yes\">M. wolinskyi<\/jats:italic>\n                        could be an emerging pathogen in device-associated infections. The case highlights the diagnostic limitations of routine methods and emphasizes the role of 16S sequencing and whole genome sequencing in accurate taxonomic classification of NTM species. Broader genomic surveillance is warranted to understand the epidemiology and taxonomy of this rapidly growing NTM.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/asmcr.00146-25","type":"journal-article","created":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T13:08:38Z","timestamp":1761052118000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["<i>Mycobacterium wolinskyi<\/i>\n                    as an emerging cause of pacemaker pocket infection and lead endocarditis: a case report and genomic characterization"],"prefix":"10.1128","volume":"2","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-9431-7763","authenticated-orcid":true,"given":"Julie","family":"Dom","sequence":"first","affiliation":[{"name":"Clinical laboratory of Microbiology, Jessa Hospital","place":["Hasselt, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinoud","family":"Cartuyvels","sequence":"additional","affiliation":[{"name":"Clinical laboratory of Microbiology, Jessa Hospital","place":["Hasselt, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Bruggemans","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Immunity, Jessa Hospital","place":["Hasselt, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timo","family":"Froyen","sequence":"additional","affiliation":[{"name":"Clinical laboratory of Molecular Microbiology, Jessa Hospital","place":["Hasselt, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra","family":"Hilkens","sequence":"additional","affiliation":[{"name":"Clinical laboratory of Microbiology, Jessa Hospital","place":["Hasselt, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koen","family":"Magerman","sequence":"additional","affiliation":[{"name":"Clinical laboratory of Microbiology, Jessa Hospital","place":["Hasselt, Belgium"]},{"id":[{"id":"https:\/\/ror.org\/04nbhqj75","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Immunology and Infection, Hasselt University","place":["Hasselt, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Martens","sequence":"additional","affiliation":[{"name":"Clinical laboratory of Microbiology, Jessa Hospital","place":["Hasselt, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Britta","family":"Van Meensel","sequence":"additional","affiliation":[{"name":"Clinical laboratory of Microbiology, Jessa Hospital","place":["Hasselt, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jozef","family":"Dingemans","sequence":"additional","affiliation":[{"name":"Clinical laboratory of Molecular Microbiology, Jessa Hospital","place":["Hasselt, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-49-4-1493"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2011.08.005"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad326"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0906412106"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-73607-x"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2021.07.003"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx343"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/507535"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-020-05385-w"},{"key":"e_1_3_1_11_2","unstructured":"LPSN. 2025. Mycobacterium Jacuzzii. LPSN. Available from: https:\/\/lpsn.dsmz.de\/species\/mycobacterium-jacuzzii"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.005585"},{"key":"e_1_3_1_13_2","unstructured":"Zhejiang University School of Medicine. 2024. Mycobacterium Wolinskii Strain: ZJ240206 Genome Sequencing [Internet]. National Library of Medicine Bethesda (MD). Available from: https:\/\/www.ncbi.nlm.nih.gov\/bioproject\/PRJNA1199355"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/9781555818814"}],"container-title":["ASM Case Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/asmcr.00146-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/asmcr.00146-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T13:43:14Z","timestamp":1767966194000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/asmcr.00146-25"}},"subtitle":[],"editor":[{"given":"Nicholas M.","family":"Moore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1,6]]},"references-count":13,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1,6]]}},"alternative-id":["10.1128\/asmcr.00146-25"],"URL":"https:\/\/doi.org\/10.1128\/asmcr.00146-25","relation":{},"ISSN":["2996-2684"],"issn-type":[{"value":"2996-2684","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,6]]},"assertion":[{"value":"2025-08-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00146-25"}}