{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T11:10:51Z","timestamp":1759057851581},"reference-count":0,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1994,3,1]],"date-time":"1994-03-01T00:00:00Z","timestamp":762480000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1994,3]]},"abstract":"<jats:p>Antibodies to human T-cell lymphotropic virus type I (HTLV-I) trans-activator protein (p40taxI) were determined in serum specimens from individuals infected with HTLV-I (n = 138) and HTLV-II (n = 19). Western blot (immunoblot) analysis using recombinant tax demonstrated the presence of anti-tax antibodies in 96% of patients (25 of 26) with HTLV-I-associated myelopathy, 43% of those (20 of 46) with adult T-cell leukemia, and 61% of asymptomatic HTLV-I blood donors (40 of 66); only one of the HTLV-II specimens reacted with the recombinant tax protein. Synthetic peptides (Tax8(106-125), Tax22(316-335), Tax-23(331-350), and Tax-24(336-353) representing the immunodominant epitopes of p40taxI detected anti-tax antibodies in 66 (48%), 50 (36%), 66 (48%), and 64 (46%) of 138 HTLV-I-positive specimens, respectively. An enzyme immunoassay using an equimolar ratio of these four peptides allowed sensitive detection of anti-tax antibodies in 96% of patients (25 of 26) with HTLV-1-associated myelopathy, 52% of adult T-cell leukemia patients (24 of 46), and 62% of asymptomatic HTLV-1-infected donors (41 of 66). The synthetic peptide-based cocktail assay was HTLV-I specific, since none of the HTLV-II-infected specimens reacted with these peptides. Interestingly, the corresponding regions from the HTLV-II tax protein, Tax8II(106-125), and Tax-22II(312-331) did not react with either HTLV-II or HTLV-I specimens. Thus, a synthetic peptide-based assay composed of immunodominant epitopes located towards the amino terminus and the C terminus of p40taxI provides a reliable and sensitive assay for the detection of anti-tax antibodies in seroepidemiologic studies.<\/jats:p>","DOI":"10.1128\/cdli.1.2.176-181.1994","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T18:33:14Z","timestamp":1578335594000},"page":"176-181","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Detection of antibodies to trans-activator protein (p40taxI) of human T-cell lymphotropic virus type I by a synthetic peptide-based assay"],"prefix":"10.1128","volume":"1","author":[{"given":"D L","family":"Rudolph","sequence":"first","affiliation":[{"name":"Retrovirus Diseases Branch, Centers for Disease Control, Atlanta, Georgia 30333, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J E","family":"Coligan","sequence":"additional","affiliation":[{"name":"Retrovirus Diseases Branch, Centers for Disease Control, Atlanta, Georgia 30333, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R B","family":"Lal","sequence":"additional","affiliation":[{"name":"Retrovirus Diseases Branch, Centers for Disease Control, Atlanta, Georgia 30333, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Clinical Diagnostic Laboratory Immunology"],"original-title":[],"language":"en","deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T05:47:08Z","timestamp":1645595228000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/cdli.1.2.176-181.1994"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,3]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,3]]}},"alternative-id":["10.1128\/cdli.1.2.176-181.1994"],"URL":"https:\/\/doi.org\/10.1128\/cdli.1.2.176-181.1994","relation":{},"ISSN":["1071-412X","1098-6588"],"issn-type":[{"value":"1071-412X","type":"print"},{"value":"1098-6588","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,3]]},"assertion":[{"value":"1994-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}