{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T10:52:58Z","timestamp":1760784778842},"reference-count":0,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1994,5,1]],"date-time":"1994-05-01T00:00:00Z","timestamp":767750400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1994,5]]},"abstract":"<jats:p>Immunophenotyping by flow cytometry and frozen-section immunoperoxidase was compared on 21 consecutive lymph node biopsy specimens, of which a diagnosis of lymphoma was made for 11 specimens. Samples for flow cytometry were obtained by a fine-needle aspiration technique. Concordance between frozen-section immunoperoxidase and flow cytometry for all routine markers on all specimens ranged from 76 to 100%. In general, B-cell markers showed poorer concordance than T-cell markers, with kappa and lambda light chains having the poorest concordance, at 76% each. Flow cytometry was significantly more sensitive (90 versus 30%; P &lt; 0.006) and had a significantly higher negative predictive value (100 versus 63%; P &lt; 0.006) than frozen-section immunoperoxidase for demonstrating light-chain restriction. There was no significant difference in the specificities (100 versus 91%) or positive predictive values (100% each) between the two methods. Both methods demonstrated characteristic immunophenotypes for intermediate cell lymphomas, small lymphocytic lymphomas, and T-cell lymphoblastic lymphomas. Frozen-section immunoperoxidase and flow cytometry appear to be significantly concordant methods for immunophenotypic analysis of lymph node biopsies. Light-chain restriction is more readily demonstrated by flow cytometry than frozen-section immunoperoxidase. We believe that ex vivo fine-needle aspiration is a simple and reliable method of obtaining cell suspensions of lymph nodes for flow cytometry.<\/jats:p>","DOI":"10.1128\/cdli.1.3.299-303.1994","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T18:33:31Z","timestamp":1578335611000},"page":"299-303","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["A comparative study of frozen-section immunoperoxidase and flow cytometry for immunophenotypic analysis of lymph node biopsies"],"prefix":"10.1128","volume":"1","author":[{"given":"K W","family":"Biesemier","sequence":"first","affiliation":[{"name":"Department of Pathology, University of North Carolina-Chapel Hill 27514, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G A","family":"Dent","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of North Carolina-Chapel Hill 27514, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K B","family":"Pryzwansky","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of North Carolina-Chapel Hill 27514, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J D","family":"Folds","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of North Carolina-Chapel Hill 27514, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Clinical Diagnostic Laboratory Immunology"],"original-title":[],"language":"en","deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T05:16:09Z","timestamp":1645593369000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/cdli.1.3.299-303.1994"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,5]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1994,5]]}},"alternative-id":["10.1128\/cdli.1.3.299-303.1994"],"URL":"https:\/\/doi.org\/10.1128\/cdli.1.3.299-303.1994","relation":{},"ISSN":["1071-412X","1098-6588"],"issn-type":[{"value":"1071-412X","type":"print"},{"value":"1098-6588","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,5]]},"assertion":[{"value":"1994-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}