{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T23:33:07Z","timestamp":1781134387320,"version":"3.54.1"},"reference-count":5,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>We investigated the effect of multiple freeze-thaw cycles on mumps, measles, and rubella virus serum antibody levels with whole-virus immunoglobulin G enzyme-linked immunoassays. Fresh serum samples from nine healthy adult volunteers were divided into six sets of five aliquots each. Samples were taken through a total of 10 freeze-thaw cycles and stored at 4\u00b0C until assayed. Each assay measurement was done in replicates of five, and the mean value was reported. After completing 10 freeze-thaw cycles, we found no clinically or statistically significant effect on measured antibody levels and found no discernible detrimental effect on the ability to measure these antibodies by enzyme-linked immunoassays.<\/jats:p>","DOI":"10.1128\/cdli.10.1.19-21.2003","type":"journal-article","created":{"date-parts":[[2003,1,9]],"date-time":"2003-01-09T23:04:09Z","timestamp":1042153449000},"page":"19-21","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["Effect of Multiple Freeze-Thaw Cycles on Detection of Measles, Mumps, and Rubella Virus Antibodies"],"prefix":"10.1128","volume":"10","author":[{"given":"Norman A.","family":"Pinsky","sequence":"first","affiliation":[{"name":"Mayo Vaccine Research Group"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeanne M.","family":"Huddleston","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert M.","family":"Jacobson","sequence":"additional","affiliation":[{"name":"Mayo Vaccine Research Group"},{"name":"Department of Pediatric and Adolescent Medicine, Mayo Clinic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter C.","family":"Wollan","sequence":"additional","affiliation":[{"name":"Olmsted Medical Group, Rochester, Minnesota 55905"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory A.","family":"Poland","sequence":"additional","affiliation":[{"name":"Mayo Vaccine Research Group"},{"name":"Department of Internal Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"1242","volume":"46","year":"1985","unstructured":"Blincoe, C., and D. W. Marble. 1985. Storage stability of some bovine plasma enzymes. Am. J. Vet. Res.46:1242-1244.","journal-title":"Am. J. Vet. Res."},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1016\/S0025-6196(12)61285-3","volume":"64","year":"1989","unstructured":"DiMagno, E. P., D. Corle, J. F. O'Brien, I. J. Masnyk, and R. Aamodt. 1989. Effect of long-term freezer storage, thawing, and refreezing on selected constituents of serum. Mayo Clin. Proc.64:1226-1234.","journal-title":"Mayo Clin. Proc."},{"key":"e_1_3_2_4_2","first-page":"308","volume":"33","year":"1987","unstructured":"Palombo, J. D., B. R. Bistrian, and G. L. Blackburn. 1987. Radioimmunoassay of apolipoprotein A-1 in serum and EDTA plasma, and effects of freeze-thaw cycles. Clin. Chem.33:308.","journal-title":"Clin. Chem."},{"key":"e_1_3_2_5_2","unstructured":"Paul J. R. and C. White. (ed.). 1973. Serologic epidemiology. Academic Press New York N.Y."},{"key":"e_1_3_2_6_2","first-page":"193","volume":"67","year":"1985","unstructured":"Petrakis, N. L. 1985. Biologic banking in cohort studies, with special reference to blood. NCI Monogr.67:193-198.","journal-title":"NCI Monogr."}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.10.1.19-21.2003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.10.1.19-21.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T21:06:59Z","timestamp":1627592819000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CDLI.10.1.19-21.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,1]]},"references-count":5,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2003,1]]}},"alternative-id":["10.1128\/CDLI.10.1.19-21.2003"],"URL":"https:\/\/doi.org\/10.1128\/cdli.10.1.19-21.2003","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,1]]},"assertion":[{"value":"2002-08-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-10-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}