{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T20:16:04Z","timestamp":1789416964759,"version":"build-2803163510"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2003,3,1]],"date-time":"2003-03-01T00:00:00Z","timestamp":1046476800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2003,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Rickettsia felis<\/jats:italic>is a flea-transmitted rickettsia. There is a discrepancy between its reported phylogenic and phenotypic identifications. Following the first report of<jats:italic>R. felis<\/jats:italic>, it was considered by tests with serologic reagents to be closely related to another recognized flea-transmitted rickettia,<jats:italic>R. typhi<\/jats:italic>. Subsequently, it appeared to be more closely related to spotted fever group (SFG) rickettsiae by genetic analysis. In the present work,<jats:italic>R. felis<\/jats:italic>was studied by microimmunofluorescence (MIF) serologic typing and with monoclonal antibodies (MAbs). Mouse polyclonal antisera to<jats:italic>R. felis<\/jats:italic>cross-reacted only with SFG rickettsiae. A neighbor-joining analysis based on MIF indicated that<jats:italic>R. felis<\/jats:italic>is actually related to SFG rickettsiae antigenically, clustering with<jats:italic>R. australis<\/jats:italic>,<jats:italic>R. akari<\/jats:italic>, and<jats:italic>R. montanensis.<\/jats:italic>A panel of 21 MAbs was raised against a 120-kDa protein antigen or a 17-kDa polypeptide of<jats:italic>R. felis<\/jats:italic>. They cross-reacted with most members of the SFG rickettsiae but not with<jats:italic>R. prowazekii<\/jats:italic>,<jats:italic>R. typhi<\/jats:italic>, or<jats:italic>R. canadensis<\/jats:italic>of the typhus group (TG) rickettsiae. Sixty-four MAbs previously generated to seven other ricketttsial species were tested with<jats:italic>R. felis.<\/jats:italic>Three MAbs reacted with the 120-kDa antigen and were generated by<jats:italic>R. africae<\/jats:italic>,<jats:italic>R. conorii<\/jats:italic>, and<jats:italic>R. akari<\/jats:italic>, respectively. They exhibited cross-reactivities with<jats:italic>R. felis<\/jats:italic>. All our data show that<jats:italic>R. felis<\/jats:italic>harbors the antigenic profile of an SFG rickettsia.<\/jats:p>","DOI":"10.1128\/cdli.10.2.221-228.2003","type":"journal-article","created":{"date-parts":[[2003,3,7]],"date-time":"2003-03-07T20:51:51Z","timestamp":1047070311000},"page":"221-228","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Antigenic Classification of<i>Rickettsia felis<\/i>by Using Monoclonal and Polyclonal Antibodies"],"prefix":"10.1128","volume":"10","author":[{"given":"Rong","family":"Fang","sequence":"first","affiliation":[{"name":"Unite\u0301 des Rickettsies, CNRS UMR 6020, IFR 48, Faculte\u0301 de Me\u0301decine, 13385 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Raoult","sequence":"additional","affiliation":[{"name":"Unite\u0301 des Rickettsies, CNRS UMR 6020, IFR 48, Faculte\u0301 de Me\u0301decine, 13385 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"400","DOI":"10.4269\/ajtmh.1990.43.400","volume":"43","year":"1990","unstructured":"Adams, J. 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