{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T22:31:44Z","timestamp":1723069904221},"reference-count":16,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2003,5,1]],"date-time":"2003-05-01T00:00:00Z","timestamp":1051747200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2003,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Current serological tests for melioidosis, using impure or uncharacterized cell antigens from\n            <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n            , have problems in detection sensitivity and specificity. Therefore, we designed and expressed the recombinant flagellin (truncated at both the N- and C-terminal ends), and used the antigen to develop an indirect enzyme-linked immunosorbent assay (ELISA) to diagnose melioidosis. Comparison of the immunoreactivities of the full-length and truncated flagellins reveals that the truncated flagellin performed much better in detection specificity and sensitivity. Only the full-length flagellin was recognized by other bacterial causing septicemia and gave a false-positive result in Western analysis, indicating that the cross-reactive epitopes were located on the more highly conserved N- and C-terminal regions of flagellin. The indirect ELISA using recombinant truncated flagellin as the antigen achieved 93.8% sensitivity and 96.3% specificity and offered a more efficient serodiagnosis of melioidosis.\n          <\/jats:p>","DOI":"10.1128\/cdli.10.3.423-425.2003","type":"journal-article","created":{"date-parts":[[2003,5,8]],"date-time":"2003-05-08T19:08:45Z","timestamp":1052420925000},"page":"423-425","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Recombinant Truncated Flagellin of\n            <i>Burkholderia pseudomallei<\/i>\n            as a Molecular Probe for Diagnosis of Melioidosis"],"prefix":"10.1128","volume":"10","author":[{"given":"Yao-Shen","family":"Chen","sequence":"first","affiliation":[{"name":"Section of Infectious Disease, Kaohsiung Veterans General Hospital"},{"name":"Institute of Biomedical Science, National Sun Yat-Sen University"},{"name":"Department of Medical Technology, Fooyin Unversity, Kaohsiung"},{"name":"Department of Life Science and Institute of Biotechnology, National Dong-Hwa University, Hualien"},{"name":"Department of Medicine, China Medical Collage, Taichung, Taiwan, Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Shiuan","sequence":"additional","affiliation":[{"name":"Section of Infectious Disease, Kaohsiung Veterans General Hospital"},{"name":"Institute of Biomedical Science, National Sun Yat-Sen University"},{"name":"Department of Medical Technology, Fooyin Unversity, Kaohsiung"},{"name":"Department of Life Science and Institute of Biotechnology, National Dong-Hwa University, Hualien"},{"name":"Department of Medicine, China Medical Collage, Taichung, Taiwan, Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ssu-Ching","family":"Chen","sequence":"additional","affiliation":[{"name":"Section of Infectious Disease, Kaohsiung Veterans General Hospital"},{"name":"Institute of Biomedical Science, National Sun Yat-Sen University"},{"name":"Department of Medical Technology, Fooyin Unversity, Kaohsiung"},{"name":"Department of Life Science and Institute of Biotechnology, National Dong-Hwa University, Hualien"},{"name":"Department of Medicine, China Medical Collage, Taichung, Taiwan, Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soi-Moi","family":"Chye","sequence":"additional","affiliation":[{"name":"Section of Infectious Disease, Kaohsiung Veterans General Hospital"},{"name":"Institute of Biomedical Science, National Sun Yat-Sen University"},{"name":"Department of Medical Technology, Fooyin Unversity, Kaohsiung"},{"name":"Department of Life Science and Institute of Biotechnology, National Dong-Hwa University, Hualien"},{"name":"Department of Medicine, China Medical Collage, Taichung, Taiwan, Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya-Lei","family":"Chen","sequence":"additional","affiliation":[{"name":"Section of Infectious Disease, Kaohsiung Veterans General Hospital"},{"name":"Institute of Biomedical Science, National Sun Yat-Sen University"},{"name":"Department of Medical Technology, Fooyin Unversity, Kaohsiung"},{"name":"Department of Life Science and Institute of Biotechnology, National Dong-Hwa University, Hualien"},{"name":"Department of Medicine, China Medical Collage, Taichung, Taiwan, Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.5.1232-1236.1993"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.14.4.361-364.1981"},{"key":"e_1_3_2_4_2","first-page":"597","volume":"13","year":"1981","unstructured":"Ashdown, L. 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