{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T01:57:53Z","timestamp":1744163873865},"reference-count":36,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2003,5,1]],"date-time":"2003-05-01T00:00:00Z","timestamp":1051747200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2003,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A recombinant protein containing the immunodominant conserved epitope region of the 56-kDa outer membrane protein of the Karp strain of\n            <jats:italic>Orientia tsutsugamushi<\/jats:italic>\n            was purified to near homogeneity using recombinant DNA techniques. The purified protein was used to immunize rabbits and produced an antibody that could recognize different strains of\n            <jats:italic>O. tsutsugamushi<\/jats:italic>\n            , as demonstrated both by Western blotting and immunofluorescence assay. An enzyme-linked immunosorbent assay (ELISA) based on this recombinant protein was developed to detect antibody (immunoglobulin G [IgG]) against\n            <jats:italic>O. tsutsugamushi<\/jats:italic>\n            in mice captured in different districts of Taiwan during 2000 to 2001. A significant difference was found in the antibody seroprevalence rates of\n            <jats:italic>Suncus murinus<\/jats:italic>\n            mice captured in different districts of Taiwan (\u03c7\n            <jats:sup>2<\/jats:sup>\n            <jats:sub>4, 0.95<\/jats:sub>\n            = 26.64;\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.05). Furthermore, a significant difference of IgG seropositivity rates was observed among different kinds of mice (\u03c7\n            <jats:sup>2<\/jats:sup>\n            <jats:sub>5, 0.95<\/jats:sub>\n            = 93.85;\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.05). Antibody seropositivity rates were higher in\n            <jats:italic>Bandicota indica<\/jats:italic>\n            (100%),\n            <jats:italic>Rattus flavipectus<\/jats:italic>\n            (96.17%), and\n            <jats:italic>Rattus losea<\/jats:italic>\n            (95.83%) than in\n            <jats:italic>Rattus norvegicus<\/jats:italic>\n            (86.05%) and\n            <jats:italic>Rattus mindanensis<\/jats:italic>\n            (83.67%) (\u03c7\n            <jats:sup>2<\/jats:sup>\n            <jats:sub>diff, 5, 0.95<\/jats:sub>\n            = 12.59,\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.05). The lowest antibody seropositivity rate (54.4%) was observed in\n            <jats:italic>Suncus murinus<\/jats:italic>\n            . Antibody seropositivity rates of mice from different districts differed significantly because of the significant difference in antibody seroprevalence rates for\n            <jats:italic>S. murinus<\/jats:italic>\n            . The results of this study indicated that the recombinant protein ELISA developed in this study could be used to conduct large-scale surveillance of rodent mice for the presence of antibody against\n            <jats:italic>O. tsutsugamushi.<\/jats:italic>\n            The high seroprevalence rates in rodent mice (except\n            <jats:italic>S. murinus<\/jats:italic>\n            ) suggest that people residing in these districts are at increased risk of developing\n            <jats:italic>O. tsutsugamushi<\/jats:italic>\n            infection.\n          <\/jats:p>","DOI":"10.1128\/cdli.10.3.451-458.2003","type":"journal-article","created":{"date-parts":[[2003,5,8]],"date-time":"2003-05-08T19:08:45Z","timestamp":1052420925000},"page":"451-458","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Development of a Recombinant Protein-Based Enzyme-Linked Immunosorbent Assay and Its Applications in Field Surveillance of Rodent Mice for Presence of Immunoglobulin G against\n            <i>Orientia tsutsugamushi<\/i>"],"prefix":"10.1128","volume":"10","author":[{"given":"Yeau-Ching","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Preventive Medicine, National Defense Medical Center, Taipei 100, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting-Yu","family":"Jian","sequence":"additional","affiliation":[{"name":"Institute of Preventive Medicine, National Defense Medical Center, Taipei 100, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lih-Jeng","family":"Tarn","sequence":"additional","affiliation":[{"name":"Institute of Preventive Medicine, National Defense Medical Center, Taipei 100, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao-Wen","family":"Hung","sequence":"additional","affiliation":[{"name":"Institute of Preventive Medicine, National Defense Medical Center, Taipei 100, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai-Yuan","family":"Chao","sequence":"additional","affiliation":[{"name":"Institute of Preventive Medicine, National Defense Medical Center, Taipei 100, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Da-Der","family":"Ji","sequence":"additional","affiliation":[{"name":"Institute of Preventive Medicine, National Defense Medical Center, Taipei 100, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hwan-Wun","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Preventive Medicine, National Defense Medical Center, Taipei 100, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Ausubel F. 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