{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T13:40:09Z","timestamp":1773668409969,"version":"3.50.1"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2003,7,1]],"date-time":"2003-07-01T00:00:00Z","timestamp":1057017600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A dipstick assay for the detection of<jats:italic>Brucella<\/jats:italic>-specific immunoglobulin M (IgM) antibodies was evaluated by studying the serological response of 133 cultures and or serologically confirmed patients with brucellosis in its different stages along with those of 34 healthy controls. As regards patients with illness less than 3 months in duration, 93.1% tested positive by the dipstick assay, a percentage similar to that obtained in the standard serum agglutination test (SAT) (92.0%), somewhat lower than that obtained by culture (100%) and higher than that obtained by IgM enzyme-linked immunosorbent assay (ELISA) (80.5%). SAT was the most sensitive test (87.0%) for patients with illness more than 3 months in duration, followed by culture (50%), the dipstick assay (28.3%), and IgM ELISA (7.5%). The results demonstrate that the dipstick assay could well be used in the serodiagnosis of patients with acute brucellosis, as well as to identify patients with a long history of the illness. Under laboratory conditions this test has the advantage of being quick and IgM antibody-specific.<\/jats:p>","DOI":"10.1128\/cdli.10.4.612-615.2003","type":"journal-article","created":{"date-parts":[[2003,7,9]],"date-time":"2003-07-09T21:36:29Z","timestamp":1057786589000},"page":"612-615","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Comparison of a Dipstick Assay for Detection of<i>Brucella<\/i>-Specific Immunoglobulin M Antibodies with Other Tests for Serodiagnosis of Human Brucellosis"],"prefix":"10.1128","volume":"10","author":[{"given":"Encarnacio\u0301n","family":"Clavijo","sequence":"first","affiliation":[{"name":"Microbiology Unit, HCU Virgen de la Victoria"},{"name":"Department of Preventive Medicine, Faculty of Medicine, Ma\u0301laga University Ma\u0301laga"},{"name":"Department of Clinical Microbiology, University of Navarra, Pamplona, Spain"},{"name":"KIT Biomedical Research, Royal Tropical Institute\/Koninklijk Instituut voor de Tropen (KIT), Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramo\u0301n","family":"Di\u0301az","sequence":"additional","affiliation":[{"name":"Microbiology Unit, HCU Virgen de la Victoria"},{"name":"Department of Preventive Medicine, Faculty of Medicine, Ma\u0301laga University Ma\u0301laga"},{"name":"Department of Clinical Microbiology, University of Navarra, Pamplona, Spain"},{"name":"KIT Biomedical Research, Royal Tropical Institute\/Koninklijk Instituut voor de Tropen (KIT), Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A\u0301ngel","family":"Anguita","sequence":"additional","affiliation":[{"name":"Microbiology Unit, HCU Virgen de la Victoria"},{"name":"Department of Preventive Medicine, Faculty of Medicine, Ma\u0301laga University Ma\u0301laga"},{"name":"Department of Clinical Microbiology, University of Navarra, Pamplona, Spain"},{"name":"KIT Biomedical Research, Royal Tropical Institute\/Koninklijk Instituut voor de Tropen (KIT), Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Garci\u0301a","sequence":"additional","affiliation":[{"name":"Microbiology Unit, HCU Virgen de la Victoria"},{"name":"Department of Preventive Medicine, Faculty of Medicine, Ma\u0301laga University Ma\u0301laga"},{"name":"Department of Clinical Microbiology, University of Navarra, Pamplona, Spain"},{"name":"KIT Biomedical Research, Royal Tropical Institute\/Koninklijk Instituut voor de Tropen (KIT), Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfonso","family":"Pinedo","sequence":"additional","affiliation":[{"name":"Microbiology Unit, HCU Virgen de la Victoria"},{"name":"Department of Preventive Medicine, Faculty of Medicine, Ma\u0301laga University Ma\u0301laga"},{"name":"Department of Clinical Microbiology, University of Navarra, Pamplona, Spain"},{"name":"KIT Biomedical Research, Royal Tropical Institute\/Koninklijk Instituut voor de Tropen (KIT), Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henk L.","family":"Smits","sequence":"additional","affiliation":[{"name":"Microbiology Unit, HCU Virgen de la Victoria"},{"name":"Department of Preventive Medicine, Faculty of Medicine, Ma\u0301laga University Ma\u0301laga"},{"name":"Department of Clinical Microbiology, University of Navarra, Pamplona, Spain"},{"name":"KIT Biomedical Research, Royal Tropical Institute\/Koninklijk Instituut voor de Tropen (KIT), Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","first-page":"65","volume":"260","year":"1985","unstructured":"Altwegg, M., and E. Bohl. 1985. Evaluation of a rapid reliable and inexpensive screening test for serological diagnosis of human brucellosis. Zentbl. Bakteriol.260:65-70.","journal-title":"Zentbl. Bakteriol."},{"key":"e_1_3_1_3_2","first-page":"1","year":"2000","unstructured":"Anonymous. 2000. 1999. Comentario epidemiol\u00f3gico de las enfermedades de declaraci\u00f3n obligatoria y sistema de informaci\u00f3n microbiol\u00f3gica espa\u00f1a. Bol. Epidemiol. Sem.I:1-5.","journal-title":"Bol. Epidemiol. Sem."},{"key":"e_1_3_1_4_2","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1097\/00001432-199604000-00017","volume":"9","year":"1996","unstructured":"Ariza, J. 1996. Brucellosis. Curr. Opin. Infect. Dis.9:126-131.","journal-title":"Brucellosis. Curr. Opin. Infect. Dis."},{"key":"e_1_3_1_5_2","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1093\/clinids\/14.1.131","volume":"14","year":"1992","unstructured":"Ariza, J., T. Pellicer, R. Pallares, A. Foz, and F. Gudio. 1992. 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Specific antibodies detected during relapse of human brucellosis. J. Infect. Dis.157:918-924.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_1_18_2","unstructured":"N. Engl. J. Med. 1965 272"},{"key":"e_1_3_1_19_2","unstructured":"Rodr\u00edguez Torres A. R. Land\u00ednez R. Her\u00e1ldez Mej\u00edas and A. Foz. 1977. Gu\u00eda pr\u00e1ctica de diagn\u00f3stico de la brucelosis humana p. 55-122. Imprenta Server-Cuesta Valladolid Spain."},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.12.4179-4182.1999"},{"key":"e_1_3_1_21_2","unstructured":"Weyant R. S. C. W. Moss. R. E. Weavwe. D. G. Hollis. J. C. Jordan. E. C. Cook and M. I. Daneshaver. 1996. Identification of unusual pathogenic gram negative aerobic and facultatively anaerobic bacteria 2nd ed. The Williams and Wilkins Co. 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