{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T15:28:41Z","timestamp":1788881321776,"version":"build-2803163510"},"reference-count":14,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2003,7,1]],"date-time":"2003-07-01T00:00:00Z","timestamp":1057017600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Sequencing of\n            <jats:italic>bp26<\/jats:italic>\n            , the gene encoding the\n            <jats:italic>Brucella<\/jats:italic>\n            sp. immunogenic BP26 periplasmic protein, was performed in the reference strains of\n            <jats:italic>Brucella abortus<\/jats:italic>\n            ,\n            <jats:italic>B. suis<\/jats:italic>\n            , and\n            <jats:italic>B. ovis<\/jats:italic>\n            . The three\n            <jats:italic>bp26<\/jats:italic>\n            sequences were almost identical to that published for\n            <jats:italic>B. melitensis<\/jats:italic>\n            16M\n            <jats:italic>bp26<\/jats:italic>\n            , and only minor nucleotide substitutions, without modifying the amino acid sequence, were observed between species. The\n            <jats:italic>bp26<\/jats:italic>\n            genes of the seven\n            <jats:italic>B. abortus<\/jats:italic>\n            biovar reference strains and\n            <jats:italic>B. abortus<\/jats:italic>\n            S19 and RB51 vaccine strains were also sequenced. Again, only minor differences were found. Surprisingly, the\n            <jats:italic>bp26<\/jats:italic>\n            nucleotide sequence for\n            <jats:italic>B. abortus<\/jats:italic>\n            S19 was almost identical to that found for\n            <jats:italic>B. melitensis<\/jats:italic>\n            16M and differed from the sequence described previously by others (O. L. Rossetti, A. I. Arese, M. L. Boschiroli, and S. L. Cravero, J. Clin. Microbiol. 34:165-169, 1996) for the same\n            <jats:italic>B. abortus<\/jats:italic>\n            strain. The epitope mapping of BP26, performed by using a panel of monoclonal antibodies and recombinant DNA techniques, allowed the identification of an immunodominant region of the protein interesting for the diagnosis of\n            <jats:italic>B. melitensis<\/jats:italic>\n            and\n            <jats:italic>B. ovis<\/jats:italic>\n            infection in sheep. A recombinant fusion protein containing this region of BP26 reacted indeed, in Western blotting, as the entire recombinant BP26 against sera from\n            <jats:italic>B. melitensis-<\/jats:italic>\n            or\n            <jats:italic>B. ovis<\/jats:italic>\n            -infected sheep while it avoided false-positive reactions observed with sera from\n            <jats:italic>Brucella<\/jats:italic>\n            -free sheep when using the entire recombinant BP26. Thus, use of this recombinant fusion protein instead the entire recombinant BP26 could improve the specific serological diagnosis of\n            <jats:italic>B. melitensis<\/jats:italic>\n            or\n            <jats:italic>B. ovis<\/jats:italic>\n            infection in sheep.\n          <\/jats:p>","DOI":"10.1128\/cdli.10.4.647-651.2003","type":"journal-article","created":{"date-parts":[[2003,7,9]],"date-time":"2003-07-09T21:36:29Z","timestamp":1057786589000},"page":"647-651","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Epitope Mapping of the\n            <i>Brucella melitensis<\/i>\n            BP26 Immunogenic Protein: Usefulness for Diagnosis of Sheep Brucellosis"],"prefix":"10.1128","volume":"10","author":[{"given":"Patricia","family":"Seco-Mediavilla","sequence":"first","affiliation":[{"name":"Departamento de Microbiologi\u0301a y Gene\u0301tica, Universidad de Salamanca, 37007 Salamanca"},{"name":"Unidad de Sanidad Animal, Servicio de Investigacio\u0301n Agroalimentaria, Diputacio\u0301n General de Arago\u0301n, 50080 Zaragoza, Spain"},{"name":"Station de Pathologie Infectieuse et Immunologie"},{"name":"Unite\u0301 BioAgresseurs, Sante\u0301 et Environnement, Institut National de la Recherche Agronomique, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Michel","family":"Verger","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a y Gene\u0301tica, Universidad de Salamanca, 37007 Salamanca"},{"name":"Unidad de Sanidad Animal, Servicio de Investigacio\u0301n Agroalimentaria, Diputacio\u0301n General de Arago\u0301n, 50080 Zaragoza, Spain"},{"name":"Station de Pathologie Infectieuse et Immunologie"},{"name":"Unite\u0301 BioAgresseurs, Sante\u0301 et Environnement, Institut National de la Recherche Agronomique, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maggy","family":"Grayon","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a y Gene\u0301tica, Universidad de Salamanca, 37007 Salamanca"},{"name":"Unidad de Sanidad Animal, Servicio de Investigacio\u0301n Agroalimentaria, Diputacio\u0301n General de Arago\u0301n, 50080 Zaragoza, Spain"},{"name":"Station de Pathologie Infectieuse et Immunologie"},{"name":"Unite\u0301 BioAgresseurs, Sante\u0301 et Environnement, Institut National de la Recherche Agronomique, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Cloeckaert","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a y Gene\u0301tica, Universidad de Salamanca, 37007 Salamanca"},{"name":"Unidad de Sanidad Animal, Servicio de Investigacio\u0301n Agroalimentaria, Diputacio\u0301n General de Arago\u0301n, 50080 Zaragoza, Spain"},{"name":"Station de Pathologie Infectieuse et Immunologie"},{"name":"Unite\u0301 BioAgresseurs, Sante\u0301 et Environnement, Institut National de la Recherche Agronomique, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara M.","family":"Mari\u0301n","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a y Gene\u0301tica, Universidad de Salamanca, 37007 Salamanca"},{"name":"Unidad de Sanidad Animal, Servicio de Investigacio\u0301n Agroalimentaria, Diputacio\u0301n General de Arago\u0301n, 50080 Zaragoza, Spain"},{"name":"Station de Pathologie Infectieuse et Immunologie"},{"name":"Unite\u0301 BioAgresseurs, Sante\u0301 et Environnement, Institut National de la Recherche Agronomique, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel S.","family":"Zygmunt","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a y Gene\u0301tica, Universidad de Salamanca, 37007 Salamanca"},{"name":"Unidad de Sanidad Animal, Servicio de Investigacio\u0301n Agroalimentaria, Diputacio\u0301n General de Arago\u0301n, 50080 Zaragoza, Spain"},{"name":"Station de Pathologie Infectieuse et Immunologie"},{"name":"Unite\u0301 BioAgresseurs, Sante\u0301 et Environnement, Institut National de la Recherche Agronomique, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Ferna\u0301ndez-Lago","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a y Gene\u0301tica, Universidad de Salamanca, 37007 Salamanca"},{"name":"Unidad de Sanidad Animal, Servicio de Investigacio\u0301n Agroalimentaria, Diputacio\u0301n General de Arago\u0301n, 50080 Zaragoza, Spain"},{"name":"Station de Pathologie Infectieuse et Immunologie"},{"name":"Unite\u0301 BioAgresseurs, Sante\u0301 et Environnement, Institut National de la Recherche Agronomique, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nieves","family":"Vizcai\u0301no","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a y Gene\u0301tica, Universidad de Salamanca, 37007 Salamanca"},{"name":"Unidad de Sanidad Animal, Servicio de Investigacio\u0301n Agroalimentaria, Diputacio\u0301n General de Arago\u0301n, 50080 Zaragoza, Spain"},{"name":"Station de Pathologie Infectieuse et Immunologie"},{"name":"Unite\u0301 BioAgresseurs, Sante\u0301 et Environnement, Institut National de la Recherche Agronomique, 37380 Nouzilly, 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