{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T15:49:33Z","timestamp":1770738573145,"version":"3.49.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2003,9,1]],"date-time":"2003-09-01T00:00:00Z","timestamp":1062374400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2003,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We evaluated industrially prepared Western blot strips designed to avoid the cross-reactions observed with indirect immunofluorescence and enzyme-linked immunosorbent assays used for the serodiagnosis of trichinellosis. The antigen preparations were crude extracts of\n            <jats:italic>Trichinella spiralis<\/jats:italic>\n            . The Western blot profile characteristic of trichinellosis was characterized by comparing 60 sera from patients infected by\n            <jats:italic>Trichinella<\/jats:italic>\n            to 11 sera from healthy subjects, 51 sera from patients with other proven parasitic diseases (cysticercosis, schistosomiasis, strongyloidosis, fascioliasis, toxocariasis, liver amebiasis, anisakiasis, filariasis, toxoplasmosis, hydatidosis, or malaria), and 23 sera from patients with autoantibodies. Specific 43- to 44-kDa and 64-kDa bands were obtained with all of the sera from 51 patients with acute trichinellosis, in 4 out of 9 patients at the early stages of the disease, and in only 1 control patient, who had suspected anisakiasis and in whom trichinellosis could not be ruled out by muscle biopsy.\n          <\/jats:p>","DOI":"10.1128\/cdli.10.5.793-796.2003","type":"journal-article","created":{"date-parts":[[2003,9,9]],"date-time":"2003-09-09T17:05:04Z","timestamp":1063127104000},"page":"793-796","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Development and Evaluation of a Western Blot Kit for Diagnosis of Human Trichinellosis"],"prefix":"10.1128","volume":"10","author":[{"given":"He\u0301le\u0300ne","family":"Yera","sequence":"first","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Centre National de Re\u0301fe\u0301rence des Trichinella Ho\u0302pital Cochin, Universite\u0301 R. Descartes, Assistance Publique-Ho\u0302pitaux de Paris, 75014 Paris"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shakir","family":"Andiva","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Centre National de Re\u0301fe\u0301rence des Trichinella Ho\u0302pital Cochin, Universite\u0301 R. Descartes, Assistance Publique-Ho\u0302pitaux de Paris, 75014 Paris"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Perret","sequence":"additional","affiliation":[{"name":"UMR BIPAR INRA AFSSA ENVA UPVM, Maisons Alfort 94700"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denis","family":"Limonne","sequence":"additional","affiliation":[{"name":"LDBIO Diagnostics, 69009 Lyon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pascal","family":"Boireau","sequence":"additional","affiliation":[{"name":"UMR BIPAR INRA AFSSA ENVA UPVM, Maisons Alfort 94700"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean","family":"Dupouy-Camet","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Centre National de Re\u0301fe\u0301rence des Trichinella Ho\u0302pital Cochin, Universite\u0301 R. Descartes, Assistance Publique-Ho\u0302pitaux de Paris, 75014 Paris"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1016\/0020-7519(93)90173-V","volume":"23","year":"1993","unstructured":"Alcantara, P., and D. Correa. 1993. Human humoral immune responses against Trichinella spiralis. Int. J. Parasitol.23:657-660.","journal-title":"Int. J. Parasitol."},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"1302","DOI":"10.1093\/oxfordjournals.aje.a114923","volume":"127","year":"1988","unstructured":"Ancelle, T., J. Dupouy-Camet, M. E. Bougnoux, V. Fourestie, H. Petit, G. Mougeot, J. P. Nozais, and J. Lapierre. 1988. Two outbreaks of trichinosis caused by horsemeat in France in 1985. Am. J. Epidemiol.127:1302-1311.","journal-title":"Am. J. Epidemiol."},{"key":"e_1_3_2_4_2","first-page":"79","volume":"60","year":"2002","unstructured":"Andiva, S., H. Yera, S. Haeghebaert, C. Tourte-Schaefer, J. F. Magnaval, and J. Dupouy-Camet. 2002. Comparative evaluation of a latex agglutination test, two ELISA tests and a Western blot test for the serodiagnosis of human trichinellosis. Ann. Biol. Clin. (Paris)60:79-83.","journal-title":"Ann. Biol. Clin. (Paris)"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1017\/S0031182000063502","volume":"92","year":"1986","unstructured":"Aronstein, W. S., S. A. Lewis, A. P. Norden, J. P. Dalton, and M. Strand. 1986. Molecular identity of a major antigen of Schistosoma mansoni which cross-reacts with Trichinella spiralis and Fasciola hepatica. Parasitology92:133-151.","journal-title":"Parasitology"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1017\/S003118200007880X","volume":"100","year":"1990","unstructured":"Bolas-Fernandez, F., and D. Wakelin. 1990. Infectivity, antigenicity and host responses to isolates of the genus Trichinella. 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