{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T16:33:27Z","timestamp":1759336407073},"reference-count":31,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2003,9,1]],"date-time":"2003-09-01T00:00:00Z","timestamp":1062374400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2003,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>trans<\/jats:italic>\n            -Sialidase is an enzyme present on the surface of\n            <jats:italic>Trypanosoma cruzi<\/jats:italic>\n            and is an important antigen recognized by sera from patients with Chagas' disease. In the present study we investigated whether the benznidazole treatment of patients with Chagas' disease induced changes in the reactivity of serum toward a recombinant form of\n            <jats:italic>trans-<\/jats:italic>\n            sialidase in order to develop an assay for monitoring of patients after treatment for Chagas' disease, which is needed at Chagas' disease control centers. By using an enzyme-linked immunosorbent assay containing a recombinant protein corresponding to the catalytic domain of\n            <jats:italic>trans-<\/jats:italic>\n            sialidase, we found that the antigen had a high specificity for sera from untreated patients with Chagas' disease. Sera from healthy individuals or patients with active visceral leishmaniasis minimally cross-reacted with the antigen. Anti-\n            <jats:italic>trans<\/jats:italic>\n            -sialidase immunoglobulin was detected in 98% of 151 untreated patients with Chagas' disease. Of these, 124 patients were treated for 60 days with benznidazole (5 mg\/kg of body weight\/day), and their sera were assayed for reactivity with the recombinant\n            <jats:italic>trans-<\/jats:italic>\n            sialidase. By using this methodology, three groups of patients could be established. The first group (60 patients), which was considered to have been successfully treated, showed no reactivity after treatment. The second group (46 patients) still showed signs of infection, and after treatment their sera recognized\n            <jats:italic>trans-<\/jats:italic>\n            sialidase, but with reduced titers. The third group (18 patients) was considered to be resistant to drug treatment, and their sera presented identical reactivities before and after treatment. These results suggest that determination of the absence of antibodies to recombinant\n            <jats:italic>trans-<\/jats:italic>\n            sialidase in treated patients by the present assay is indicative of treatment success, while the presence of antibodies may indicate the persistence of infection. Therefore, this method may be useful for the diagnosis and monitoring of patients undergoing benznidazole treatment.\n          <\/jats:p>","DOI":"10.1128\/cdli.10.5.826-830.2003","type":"journal-article","created":{"date-parts":[[2003,9,9]],"date-time":"2003-09-09T17:05:04Z","timestamp":1063127104000},"page":"826-830","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Enzyme-Linked Immunoassay Using Recombinant\n            <i>trans<\/i>\n            -Sialidase of\n            <i>Trypanosoma cruzi<\/i>\n            Can Be Employed for Monitoring of Patients with Chagas' Disease after Drug Treatment"],"prefix":"10.1128","volume":"10","author":[{"given":"Vera Lucia","family":"Pereira-Chioccola","sequence":"first","affiliation":[{"name":"Laborato\u0301rio de Parasitologia, Instituto Adolfo Lutz"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abilio Augusto","family":"Fragata-Filho","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Xenodiagno\u0301stico, Instituto Dante Pazzanese de Cardiologia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio Marcos de Apparecida","family":"Levy","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Xenodiagno\u0301stico, Instituto Dante Pazzanese de Cardiologia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauricio M.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia, UNIFESP, Sa\u0303o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"Schenkman","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia, UNIFESP, Sa\u0303o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"850","volume":"37","year":"1993","unstructured":"Almeida, I. 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