{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T04:31:00Z","timestamp":1719808260850},"reference-count":18,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2003,9,1]],"date-time":"2003-09-01T00:00:00Z","timestamp":1062374400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2003,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The ability to use mitochondrial heat shock protein 70 (MTP) of<jats:italic>Trypanosoma congolense<\/jats:italic>as a diagnostic antigen was examined. One cDNA clone was obtained by immunoscreening of a<jats:italic>T. congolense<\/jats:italic>procyclic form (PCF) cDNA library with monoclonal antibody (MAb) 10F9. The cDNA clone contained an open reading frame of 1,977 bp encoding a polypeptide consisting of 659 amino acids. Southern blotting analysis indicated that there were at least three copies of the<jats:italic>MTP<\/jats:italic>gene in the haploid genome. Interference of the MTP RNA resulted in complete inhibition, which indicated that MTP is essential at the PCF stage. Northern and Western blotting analyses revealed that MTP is expressed both in the bloodstream form (BSF) and in PCF. The B-cell epitope recognized by MAb 10F9 was located within 206 amino acids from the C terminus. Depending on the conditions of protein extraction, MTP was cleaved into smaller polypeptides by endogenous proteases. However, the C-terminal epitope of MTP was preserved with a high degree of antigenicity, even after cleavage. Antibody detection by enzyme-linked immunosorbent assay with the truncated recombinant MTP revealed that anti-MTP antibodies exist in experimentally infected mouse sera. Thus, MTP may be useful as an antigen for the serodiagnosis of primary<jats:italic>T. congolense<\/jats:italic>infection.<\/jats:p>","DOI":"10.1128\/cdli.10.5.926-933.2003","type":"journal-article","created":{"date-parts":[[2003,9,9]],"date-time":"2003-09-09T17:05:04Z","timestamp":1063127104000},"page":"926-933","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Cloning and Expression of Mitochondrial Heat Shock Protein 70 of<i>Trypanosoma congolense<\/i>and Potential Use as a Diagnostic Antigen"],"prefix":"10.1128","volume":"10","author":[{"given":"Hiroshi","family":"Bannai","sequence":"first","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuya","family":"Sakurai","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noboru","family":"Inoue","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chihiro","family":"Sugimoto","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ikuo","family":"Igarashi","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1006\/expr.2001.4667","volume":"100","year":"2002","unstructured":"Boulange, A., J. Katende, and E. Anthie. 2002. Trypanosoma congolense: expression of a heat shock protein 70 and initial evaluation as a diagnostic antigen for bovine trypanosomosis. Exp. Parasitol.100:6-11.","journal-title":"Exp. Parasitol."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.9.4893-4899.2000"},{"key":"e_1_3_2_4_2","first-page":"1017","volume":"88","year":"2002","unstructured":"Chen, M., F. Aosai, K. Norose, H. S. Mun, O. Takeuchi, S. Akira, and A. Yano. 2002. Involvement of MyD88 in host defense and the down-regulation of anti-heat shock protein 70 autoantibody formation by MyD88 in Toxoplasma gondii-infected mice. J. Parasitol.88:1017-1019.","journal-title":"J. Parasitol."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S0166-6851(97)00222-3","volume":"92","year":"1998","unstructured":"Chi, T. B., V. Silvia, B. Brown, and N. Williams. 1998. Subunit 9 of the mitochondrial ATP synthase of Trypanosoma brucei is nuclearly encoded and developmentally regulated. Mol. Biochem. Parasitol.92:29-38.","journal-title":"Mol. Biochem. Parasitol."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.13.6.3598-3610.1993"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"3987","DOI":"10.4049\/jimmunol.144.10.3987","volume":"144","year":"1990","unstructured":"Engman, D. M., E. A. Dragon, and J. E. Donelson. 1990. Human humoral immunity to hsp70 during Trypanosoma cruzi infection. J. Immunol.144:3987-3991.","journal-title":"J. Immunol."},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/0166-6851(92)90210-B","volume":"51","year":"1992","unstructured":"Engman, D. M., S. Fehr, and J. E. Donelson. 1992. Specific functional domains of mitochondrial hsp70s suggested by sequence comparison of the trypanosome and yeast proteins. Mol. Biochem. Parasitol.51:153-156.","journal-title":"Mol. Biochem. Parasitol."},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/S0304-4017(97)00134-9","volume":"73","year":"1997","unstructured":"Greiner, M., S. Kumar, and C. Kyeswa. 1997. Evaluation and comparison of antibody ELISAs for serodiagnosis of bovine trypanosomosis. Vet. Parasitol.73:197-205.","journal-title":"Vet. Parasitol."},{"key":"e_1_3_2_10_2","first-page":"240","volume":"73","year":"1997","unstructured":"Hausler, T., Y. D. Stierhof, J. Blattner, and C. Clayton. 1997. Conservation of mitochondrial targeting sequence function in mitochondrial and hydrogenosomal proteins from the early-branching eukaryotes Crithidia,Trypanosoma and Trichomonas. Eur. J. Cell Biol.73:240-251.","journal-title":"Eur. J. Cell Biol."},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1017\/S0031182000062533","volume":"102","year":"1991","unstructured":"Hirumi, H., and K. Hirumi. 1991. In vitro cultivation of Trypanosoma congolense bloodstream forms in the absence of feeder cell layer. Parasitology102:225-236.","journal-title":"Parasitology"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1292\/jvms.62.1041","volume":"62","year":"2000","unstructured":"Inoue, N., A. T. Lluz, T. Mori, H. Nagasawa, K. Fujisaki, and T. Mikami. 2000. Novel species specific antigens of Trypanosoma congolense and their different localization among life-cycle stages. J. Vet. Med. Sci.62:1041-1045.","journal-title":"J. Vet. Med. Sci."},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/S0166-6851(02)00015-4","volume":"120","year":"2002","unstructured":"Inoue, N., K. Otsu, D. M. Ferraro, and J. E. Donelson. 2002. Tetracycline-regulated RNA interference in Trypanosoma congolense. Mol. Biochem. Parasitol.120:309-313.","journal-title":"Mol. Biochem. Parasitol."},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/S0166-6851(00)00300-5","volume":"111","year":"2000","unstructured":"LaCount, D. J., S. Bruse, K. L. Hill, and J. E. Donelson. 2000. Double-strand RNA interference in Trypanosoma brucei using head-to-head promoters. Mol. Biochem. Parasitol.111:67-76.","journal-title":"Mol. Biochem. Parasitol."},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/0014-4894(70)90120-7","volume":"28","year":"1970","unstructured":"Lanham, S. M., and D. G. Godfrey. 1970. Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose. Exp. Parasitol.28:521-534.","journal-title":"Exp. Parasitol."},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"A38","DOI":"10.1016\/S0167-5699(05)80011-8","volume":"12","year":"1991","unstructured":"Polla, B. S. 1991. Heat shock proteins in host-parasite interactions. Immunol. Today12:A38-A41.","journal-title":"Immunol. Today"},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/0166-6851(92)90022-C","volume":"53","year":"1992","unstructured":"Requena, J. M., A. Jimenez-Ruiz, M. Soto, R. Assiego, J. F. Santaren, M. C. Lopez, M. E. Patarroyo, and C. Alonso. 1992. Regulation of hsp70 expression in Trypanosoma cruzi by temperature and growth phase. Mol. Biochem. Parasitol.53:201-211.","journal-title":"Mol. Biochem. Parasitol."},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1242\/jcs.104.4.1091","volume":"104","year":"1993","unstructured":"Searle, S., M. V. McCrossan, and D. F. Smith. 1993. Expression of mitochondrial stress protein in the protozoan parasite Leishmania major. J. Cell Sci.104:1091-1100.","journal-title":"J. Cell Sci."},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/S0166-6851(01)00282-1","volume":"115","year":"2001","unstructured":"Silvia, V., B. Brown, T. B. Chi, and N. Williams. 2001. The Trypanosoma brucei mitochondrial ATP synthase is developmentally regulated at the level of transcript stability. Mol. Biochem. Parasitol.115:177-187.","journal-title":"Mol. Biochem. Parasitol."}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.10.5.926-933.2003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.10.5.926-933.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,26]],"date-time":"2023-04-26T21:55:37Z","timestamp":1682546137000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CDLI.10.5.926-933.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,9]]},"references-count":18,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2003,9]]}},"alternative-id":["10.1128\/CDLI.10.5.926-933.2003"],"URL":"https:\/\/doi.org\/10.1128\/cdli.10.5.926-933.2003","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,9]]},"assertion":[{"value":"2002-12-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-06-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}