{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:18:31Z","timestamp":1792801111085,"version":"4.5.0"},"reference-count":11,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Group 3 of outer membrane proteins (OMPs) of<jats:italic>Brucella<\/jats:italic>includes Omp25 and Omp31, which share 34% identity. Omp25 is highly conserved in<jats:italic>Brucella<\/jats:italic>species, and Omp31 is present in all<jats:italic>Brucella<\/jats:italic>species, except<jats:italic>Brucella abortus<\/jats:italic>. Antibodies to<jats:italic>Brucella melitensis<\/jats:italic>Omp31 have been sought only in infected sheep, and Western blotting of sera from infected sheep did not reveal anti-Omp31 reactivity. We obtained recombinant purified Omp31 (<jats:italic>B. melitensis<\/jats:italic>) and tested its recognition by sera from humans and animals suffering from brucellosis by an indirect enzyme-linked immunosorbent assay (ELISA). Serum samples from 74 patients, 57 sheep, and 47 dogs were analyzed; brucellosis was confirmed by bacteriological isolation in all ovine and canine cases and 31 human cases of brucellosis. Thirty-five patients (47%) were positive for antibodies to Omp31, including seven cases of<jats:italic>Brucella suis<\/jats:italic>infection, two cases of<jats:italic>B. abortus<\/jats:italic>infection, and three cases of<jats:italic>B. melitensis<\/jats:italic>infection. Of 39 sheep naturally infected with<jats:italic>B. melitensis<\/jats:italic>(biovars 1 and 3), 23 (59%) were positive for antibodies to Omp31. Anti-Omp31 antibodies were also detected in 12 of 18 rams (67%) in which<jats:italic>Brucella ovis<\/jats:italic>was isolated from semen. Antibodies to Omp31 were also found in 41 (87%) of the 47 dogs, including 13 with recent infection. These results suggest that an indirect ELISA using recombinant purified Omp31 from<jats:italic>B. melitensis<\/jats:italic>would be of limited value for the diagnosis of human and animal brucellosis. Nevertheless, the potential usefulness of this antigen in combination with other recombinant proteins from<jats:italic>Brucella<\/jats:italic>should not be dismissed.<\/jats:p>","DOI":"10.1128\/cdli.11.1.111-114.2004","type":"journal-article","created":{"date-parts":[[2004,1,11]],"date-time":"2004-01-11T00:18:20Z","timestamp":1073780300000},"page":"111-114","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Antibody Reactivity to Omp31 from<i>Brucella melitensis<\/i>in Human and Animal Infections by Smooth and Rough Brucellae"],"prefix":"10.1128","volume":"11","author":[{"given":"Juliana","family":"Cassataro","sequence":"first","affiliation":[{"name":"Laboratorio de Inmunogene\u0301tica, Hospital de Cli\u0301nicas"},{"name":"Instituto de Estudios de la Inmunidad Humoral, Facultad de Farmacia y Bioqui\u0301mica, Universidad de Buenos Aires"},{"name":"Servicio de Brucelosis, Hospital F.J. Mun\u0303iz, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karina","family":"Pasquevich","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunogene\u0301tica, Hospital de Cli\u0301nicas"},{"name":"Instituto de Estudios de la Inmunidad Humoral, Facultad de Farmacia y Bioqui\u0301mica, Universidad de Buenos Aires"},{"name":"Servicio de Brucelosis, Hospital F.J. Mun\u0303iz, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Bruno","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunogene\u0301tica, Hospital de Cli\u0301nicas"},{"name":"Instituto de Estudios de la Inmunidad Humoral, Facultad de Farmacia y Bioqui\u0301mica, Universidad de Buenos Aires"},{"name":"Servicio de Brucelosis, Hospital F.J. Mun\u0303iz, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge C.","family":"Wallach","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunogene\u0301tica, Hospital de Cli\u0301nicas"},{"name":"Instituto de Estudios de la Inmunidad Humoral, Facultad de Farmacia y Bioqui\u0301mica, Universidad de Buenos Aires"},{"name":"Servicio de Brucelosis, Hospital F.J. Mun\u0303iz, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos A.","family":"Fossati","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunogene\u0301tica, Hospital de Cli\u0301nicas"},{"name":"Instituto de Estudios de la Inmunidad Humoral, Facultad de Farmacia y Bioqui\u0301mica, Universidad de Buenos Aires"},{"name":"Servicio de Brucelosis, Hospital F.J. Mun\u0303iz, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo C.","family":"Baldi","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunogene\u0301tica, Hospital de Cli\u0301nicas"},{"name":"Instituto de Estudios de la Inmunidad Humoral, Facultad de Farmacia y Bioqui\u0301mica, Universidad de Buenos Aires"},{"name":"Servicio de Brucelosis, Hospital F.J. Mun\u0303iz, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/S0378-1135(97)00134-X","volume":"57","year":"1997","unstructured":"Baldi, P. C., M. M. Wanke, M. E. Loza, N. Monachesi, and C. A. Fossati. 1997. Diagnosis of canine brucellosis by detection of IgG antibodies against an 18 kDa cytoplasmic protein of Brucella spp. Vet. Microbiol.57:273-281.","journal-title":"Vet. Microbiol."},{"key":"e_1_3_2_3_2","first-page":"366","volume":"9","year":"2002","unstructured":"Cassataro, J., M. V. Delpino, C. A. Velikovsky, L. Bruno, C. A. Fossati, and P. C. Baldi. 2002. Diagnostic usefulness of antibodies against the ribosome recycling factor from Brucella melitensis in human or canine brucellosis. Clin. Diagn. Lab. Immunol.9:366-369.","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.12.3980-3987.1990"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/S0378-1135(02)00211-0","volume":"90","year":"2002","unstructured":"Cloeckaert, A., N. Vizca\u00edno, J. Y. Paquet, R. A. Bowden, and P. H. Elzer. 2002. Major outer membrane proteins of Brucella spp.: past, present and future. Vet. Microbiol.90:229-247.","journal-title":"Vet. Microbiol."},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S1286-4579(02)00075-8","volume":"5","year":"2003","unstructured":"Estein, S. M., J. Cassataro, N. Vizca\u00edno, M. S. Zygmunt, A. Cloeckaert, and R. A. Bowden. 2003. The recombinant Omp31 from Brucella melitensis alone or associated with rough lipopolysaccharide induces protection against Brucella ovis infection in BALB\/c mice. Microbes Infect.5:85-93.","journal-title":"Microbes Infect."},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S0378-1135(97)00196-X","volume":"59","year":"1998","unstructured":"Kittelberger, R., D. S. Diack, N. Vizca\u00edno, M. S. Zygmunt, and A. Cloeckaert. 1998. Characterization of an immunodominant antigen in Brucella ovis and evaluation of its use in an enzyme-linked immunosorbent assay. Vet. Microbiol.59:213-227.","journal-title":"Vet. 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DNA polymorphism at the omp-31 locus of Brucella spp.: evidence for a large deletion in Brucella abortus, and other species-specific markers. Microbiology143:2913-2921.","journal-title":"Microbiology"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.11.7020-7028.2001"}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.11.1.111-114.2004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.11.1.111-114.2004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,14]],"date-time":"2024-12-14T21:54:36Z","timestamp":1734213276000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CDLI.11.1.111-114.2004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1]]},"references-count":11,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,1]]}},"alternative-id":["10.1128\/CDLI.11.1.111-114.2004"],"URL":"https:\/\/doi.org\/10.1128\/cdli.11.1.111-114.2004","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,1]]},"assertion":[{"value":"2003-09-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-11-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}