{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:52:56Z","timestamp":1777380776948,"version":"3.51.4"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Finding evidence of anisakidosis requires invasive methods. We have developed a serological assay based on the detection of an immunoglobulin E (IgE) specifically directed against Ani s 1 protein, a major parasite allergen of\n            <jats:italic>Anisakis simplex<\/jats:italic>\n            , which has shown a high level of accuracy in the diagnosis of anisakidosis. We used this tool to determine the prevalence of anti-Ani s 1 IgE in dyspeptic patients and to investigate if its seropositivity could be related to epidemiological factors other than raw fish consumption. A total of 174 dyspeptic patients who submitted to upper digestive tract endoscopy were studied. Specific IgE against Ani s 1 was determined by immunoblotting. Quantitative information on smoking, alcohol consumption, and fish consumption as well as a history of gastric surgery was recorded. Twenty-four (13.8%) patients were seropositive for Ani s 1 protein. The seroprevalence of anti-Ani s 1 IgE increased with age in patients who were less than 62 years old (\n            <jats:italic>P<\/jats:italic>\n            = 0.047). Seropositivity to Ani s 1 was associated with the consumption of fish in vinegar (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001), raw fish (\n            <jats:italic>P<\/jats:italic>\n            = 0.001), and smoked fish (\n            <jats:italic>P<\/jats:italic>\n            = 0.007). There was no relationship between anti-Ani s 1 IgE seropositivity and the number of cigarettes smoked (\n            <jats:italic>P<\/jats:italic>\n            = 0.098) or alcohol intake (\n            <jats:italic>P<\/jats:italic>\n            = 0.179). Five patients had undergone previous gastric surgery, and three of those patients were seropositive for Ani s 1 (\n            <jats:italic>P<\/jats:italic>\n            = 0.019). In multivariate analysis, the consumption of fish in vinegar (\n            <jats:italic>P<\/jats:italic>\n            = 0.006), raw fish (\n            <jats:italic>P<\/jats:italic>\n            = 0.017), and smoked fish (\n            <jats:italic>P<\/jats:italic>\n            = 0.002) and a history of gastric surgery (\n            <jats:italic>P<\/jats:italic>\n            = 0.005) were independent factors associated with anti-Ani s 1 IgE detection. In conclusion, at present, anisakidosis might frequently be underdiagnosed, and it might have a clinical role in patients with upper dyspeptic symptoms. Uncooked-fish ingestion and previous gastric surgery were associated with seropositivity for Ani s 1 protein.\n          <\/jats:p>","DOI":"10.1128\/cdli.11.1.115-118.2004","type":"journal-article","created":{"date-parts":[[2004,1,11]],"date-time":"2004-01-11T00:18:20Z","timestamp":1073780300000},"page":"115-118","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["High Prevalence of Seropositivity to a Major Allergen of\n            <i>Anisakis simplex<\/i>\n            , Ani s 1, in Dyspeptic Patients"],"prefix":"10.1128","volume":"11","author":[{"given":"Carlos","family":"Toro","sequence":"first","affiliation":[{"name":"Services of Infectious Diseases"},{"name":"Immunology"},{"name":"Microbiology"},{"name":"Gastroenterology"},{"name":"Pathology, Hospital Carlos III"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mari\u0301a Luisa","family":"Caballero","sequence":"additional","affiliation":[{"name":"Services of Infectious Diseases"},{"name":"Immunology"},{"name":"Microbiology"},{"name":"Gastroenterology"},{"name":"Pathology, Hospital Carlos III"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margarita","family":"Baquero","sequence":"additional","affiliation":[{"name":"Services of Infectious Diseases"},{"name":"Immunology"},{"name":"Microbiology"},{"name":"Gastroenterology"},{"name":"Pathology, Hospital Carlos III"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"Garci\u0301a-Samaniego","sequence":"additional","affiliation":[{"name":"Services of Infectious Diseases"},{"name":"Immunology"},{"name":"Microbiology"},{"name":"Gastroenterology"},{"name":"Pathology, Hospital Carlos III"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabel","family":"Casado","sequence":"additional","affiliation":[{"name":"Services of Infectious Diseases"},{"name":"Immunology"},{"name":"Microbiology"},{"name":"Gastroenterology"},{"name":"Pathology, Hospital Carlos III"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margarita","family":"Rubio","sequence":"additional","affiliation":[{"name":"Services of Infectious Diseases"},{"name":"Immunology"},{"name":"Microbiology"},{"name":"Gastroenterology"},{"name":"Pathology, Hospital Carlos III"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ignacio","family":"Moneo","sequence":"additional","affiliation":[{"name":"Services of Infectious Diseases"},{"name":"Immunology"},{"name":"Microbiology"},{"name":"Gastroenterology"},{"name":"Pathology, Hospital Carlos III"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1007\/s004360050120","volume":"82","year":"1996","unstructured":"Adroher, F. J., A. Valero, J. Ruiz-Valero, and L. Iglesias. 1996. Larval anisakids (Nematoda: Ascaridoidea) in horse mackerel (Trachurus trachurus) from the fish market in Granada, Spain. Parasitol. Res.82:319-322.","journal-title":"Parasitol. Res."},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/S0304-4017(99)00037-0","volume":"84","year":"1999","unstructured":"Ashton, F. H., J. Li, and G. A. Schad. 1999. Chemo- and thermosensory neurons: structure and function in animal parasitic nematodes. Vet. Parasitol.84:297-316.","journal-title":"Vet. Parasitol."},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/S1471-4922(01)02152-3","volume":"18","year":"2002","unstructured":"Audicana, M. T., I. J. Ansotegui, L. Fern\u00e1ndez de Corres, and M. W. Kennedy. 2002. Anisakis simplex: dangerous\u2014dead and alive? 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Gut29:843-847.","journal-title":"Gut"}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.11.1.115-118.2004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.11.1.115-118.2004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T20:34:04Z","timestamp":1627590844000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CDLI.11.1.115-118.2004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,1]]}},"alternative-id":["10.1128\/CDLI.11.1.115-118.2004"],"URL":"https:\/\/doi.org\/10.1128\/cdli.11.1.115-118.2004","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,1]]},"assertion":[{"value":"2003-07-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-10-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}