{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T19:58:16Z","timestamp":1790193496060,"version":"4.1.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2004,3,1]],"date-time":"2004-03-01T00:00:00Z","timestamp":1078099200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2004,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A rapid and simple method for the simultaneous quantitation of serum immunoglobulin G (IgG) antibodies specific for\n            <jats:italic>Neisseria meningitidis<\/jats:italic>\n            serogroups A, C, Y, and W-135 was developed and evaluated. Four bead sets were generated, each conjugated with one of the meningococcal capsular polysaccharides (A, C, Y, or W-135) and serologically assessed by the use of antimeningococcal international reference sera. Cross-reactivity studies demonstrated no inhibition between monoplex and multiplex assays, and the assay was linear over a 24-fold serum dilution range. Inhibition studies demonstrated that the assay is specific, with &lt;25% heterologous inhibition occurring. The assay was also found to have low intra- and interassay variations and limits of detection \u2264650 pg\/ml. A comparison of the meningococcal bead assay with the standardized meningococcal enzyme-linked immunosorbent assay showed a good correlation between the IgG concentrations obtained by each assay. The tetraplex assay has the potential to be an important addition to the serologic evaluation of meningococcal capsular polysaccharide conjugate vaccines.\n          <\/jats:p>","DOI":"10.1128\/cdli.11.2.272-279.2004","type":"journal-article","created":{"date-parts":[[2004,3,10]],"date-time":"2004-03-10T20:13:13Z","timestamp":1078949593000},"page":"272-279","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":58,"title":["Development and Evaluation of a Tetraplex Flow Cytometric Assay for Quantitation of Serum Antibodies to\n            <i>Neisseria meningitidis<\/i>\n            Serogroups A, C, Y, and W-135"],"prefix":"10.1128","volume":"11","author":[{"given":"Gouri","family":"Lal","sequence":"first","affiliation":[{"name":"Health Protection Agency, Vaccine Evaluation Department, Manchester Royal Infirmary"},{"name":"Manchester Metropolitan University, Manchester, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Balmer","sequence":"additional","affiliation":[{"name":"Health Protection Agency, Vaccine Evaluation Department, Manchester Royal Infirmary"},{"name":"Manchester Metropolitan University, Manchester, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"Joseph","sequence":"additional","affiliation":[{"name":"Health Protection Agency, Vaccine Evaluation Department, Manchester Royal Infirmary"},{"name":"Manchester Metropolitan University, Manchester, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maureen","family":"Dawson","sequence":"additional","affiliation":[{"name":"Health Protection Agency, Vaccine Evaluation Department, Manchester Royal Infirmary"},{"name":"Manchester Metropolitan University, Manchester, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ray","family":"Borrow","sequence":"additional","affiliation":[{"name":"Health Protection Agency, Vaccine Evaluation Department, Manchester Royal Infirmary"},{"name":"Manchester Metropolitan University, Manchester, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.1086\/344324","volume":"186","year":"2002","unstructured":"Borrow, R., D. Goldblatt, N. Andrews, J. Southern, L. Ashton, S. Deane, R. Morris, K. Cartwright, and E. Miller. 2002. Antibody persistence and immunological memory at age 4 years after meningococcal group C conjugate vaccination in children in the United Kingdom. J. Infect. Dis.186:1353-1357.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1111\/j.1574-695X.2000.tb01460.x","volume":"28","year":"2000","unstructured":"Borrow, R., P. Richmond, E. B. Kaczmarski, A. Iverson, S. L. Martin, J. Findlow, M. Acuna, E. Longworth, R. O'Connor, J. Paul, and E. Miller. 2000. Meningococcal serogroup C-specific IgG antibody responses and serum bactericidal titers in children following vaccination with a meningococcal A\/C polysaccharide vaccine. FEMS Immunol. Med. Microbiol.28:79-85.","journal-title":"FEMS Immunol. Med. 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