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Elevated levels of total immunoglobulin E (IgE), specific IgE, and IgG antibodies in sera are important immunodiagnostic criteria for ABPA. International reference standards or standardized immunodiagnostic assays are not available due to a lack of well-defined diagnostic antigens. The present study was carried out to identify and evaluate the immunodiagnostic relevance of synthetic epitopic peptides of Asp f 1, a major allergen, antigen, or cytotoxin of<jats:italic>A. fumigatus<\/jats:italic>. Five overlapping peptides were synthesized from the N terminus of Asp f 1, one of the potential immunodominant regions predicted by algorithmic programs. The 11-amino-acid synthetic peptide (P1) significantly inhibited both IgG binding (89.10% \u00b1 4.45%) and IgE binding (77.32% \u00b1 3.38%) of the standardized diagnostic antigen (SDA) (a well-defined pool of diagnostically relevant allergens and antigens of<jats:italic>A. fumigatus<\/jats:italic>). With a panel of sera of ABPA patients, allergic patients with skin test negativity to<jats:italic>A. fumigatus<\/jats:italic>, and healthy individuals, P1 showed a higher diagnostic efficiency than SDA (specific IgG, 100%; specific IgE, 98.3%). The diagnostic efficiency of P1 could be attributed to the presence of homologous epitopes in various immunodominant allergens or antigens of<jats:italic>A. fumigatus<\/jats:italic>. The ability of P1 to induce histamine release from sensitized mast cells and a Th2 type of cytokine profile in peripheral blood mononuclear cells of ABPA patients suggests its potential for use in intradermal testing. P1 could be further explored for development of a standardized, specific, and sensitive immunodiagnostic test for aspergillosis.<\/jats:p>","DOI":"10.1128\/cdli.11.3.552-558.2004","type":"journal-article","created":{"date-parts":[[2004,5,11]],"date-time":"2004-05-11T19:33:03Z","timestamp":1084303983000},"page":"552-558","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Use of a Synthetic Peptide Epitope of Asp f 1, a Major Allergen or Antigen of<i>Aspergillus fumigatus<\/i>, for Improved Immunodiagnosis of Allergic Bronchopulmonary Aspergillosis"],"prefix":"10.1128","volume":"11","author":[{"given":"Taruna","family":"Madan","sequence":"first","affiliation":[{"name":"Molecular Biochemistry and Diagnostics, Institute of Genomics and Integrative Biology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priyanka","family":"Priyadarsiny","sequence":"additional","affiliation":[{"name":"Molecular Biochemistry and Diagnostics, Institute of Genomics and Integrative Biology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mudit","family":"Vaid","sequence":"additional","affiliation":[{"name":"Molecular Biochemistry and Diagnostics, Institute of Genomics and Integrative Biology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neel","family":"Kamal","sequence":"additional","affiliation":[{"name":"Molecular Biochemistry and Diagnostics, Institute of Genomics and Integrative Biology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashok","family":"Shah","sequence":"additional","affiliation":[{"name":"Clinical Research Centre, Vallabh Bhai Patel Chest Institute, University of Delhi, Delhi"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wahajul","family":"Haq","sequence":"additional","affiliation":[{"name":"Biopolymers Division, Central Drug Research Institute, Lucknow, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seturam Bandacharya","family":"Katti","sequence":"additional","affiliation":[{"name":"Biopolymers Division, Central Drug Research Institute, Lucknow, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. 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