{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T05:41:26Z","timestamp":1790228486718,"version":"4.1.0"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2004,5,1]],"date-time":"2004-05-01T00:00:00Z","timestamp":1083369600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2004,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Protein-energy malnutrition is the primary cause of immune deficiency in children across the world. It has been related to changes in peripheral T-lymphocyte subsets. The aim of the present study was to evaluate the effects of infection and malnutrition on the proportion of peripheral-lymphocyte subsets in well-nourished non-bacterium-infected (WN), well-nourished bacterium-infected (WNI), and malnourished bacterium-infected (MNI) children by flow cytometry. A prospectively monitored cohort of 15 MNI, 12 WNI, and 17 WN children was studied. All the children were 3 years old or younger and had only bacterial infections. Results showed a significant decrease in the proportion of T CD3\n            <jats:sup>+<\/jats:sup>\n            (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.05 for relative and\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.03 for absolute values), CD4\n            <jats:sup>+<\/jats:sup>\n            (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.01 for relative and absolute values), and CD8\n            <jats:sup>+<\/jats:sup>\n            (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.05 for relative values) lymphocyte subsets in WNI children compared to the results seen with WN children. Additionally, B lymphocytes in MNI children showed significant lower values (CD20\n            <jats:sup>+<\/jats:sup>\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.02 for relative and\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.05 for absolute values) in relation to the results seen with WNI children. These results suggest that the decreased proportions of T-lymphocyte subsets observed in WNI children were associated with infection diseases and that the incapacity to increase the proportion of B lymphocyte was associated with malnutrition. This low proportion of B lymphocytes may be associated with the mechanisms involved in the immunodeficiency of malnourished children.\n          <\/jats:p>","DOI":"10.1128\/cdli.11.3.577-580.2004","type":"journal-article","created":{"date-parts":[[2004,5,11]],"date-time":"2004-05-11T19:33:03Z","timestamp":1084303983000},"page":"577-580","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":60,"title":["Flow Cytometry Study of Lymphocyte Subsets in Malnourished and Well-Nourished Children with Bacterial Infections"],"prefix":"10.1128","volume":"11","author":[{"given":"Oralia","family":"Na\u0301jera","sequence":"first","affiliation":[{"name":"Departamento de Atencio\u0301n a la Salud Universidad Auto\u0301noma Metropolitana\u2014Xochimilco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Gonza\u0301lez","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias de la Salud, Universidad Auto\u0301noma Metropolitana\u2014Iztapalapa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guadalupe","family":"Toledo","sequence":"additional","affiliation":[{"name":"Hospital Materno-Pedia\u0301trico, Xochimilco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Lo\u0301pez","sequence":"additional","affiliation":[{"name":"Secretari\u0301a de Salud del Gobierno del Distrito Federal, Iztapalapa, Me\u0301xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roci\u0301o","family":"Ortiz","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias de la Salud, Universidad Auto\u0301noma Metropolitana\u2014Iztapalapa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"235","volume":"60","year":"2003","unstructured":"Arredondo-Garc\u00eda, J. 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