{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T12:06:04Z","timestamp":1764936364582},"reference-count":33,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2004,9,1]],"date-time":"2004-09-01T00:00:00Z","timestamp":1093996800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2004,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>White-tailed deer (<jats:italic>Odocoileus virginianus<\/jats:italic>) have emerged as reservoirs of bovine tuberculosis in northern America. For tuberculosis surveillance of deer, antibody-based assays are particularly attractive because deer are handled only once and immediate processing of the sample is not required. Sera collected sequentially from 25<jats:italic>Mycobacterium bovis<\/jats:italic>-infected and 7 noninfected deer were evaluated by enzyme-linked immunosorbent assay (ELISA), immunoblotting, and multiantigen print immunoassay (MAPIA) for immunoglobulin specific to<jats:italic>M. bovis<\/jats:italic>antigens. Various routes of experimental<jats:italic>M. bovis<\/jats:italic>infection, such as intratonsillar inoculation (<jats:italic>n<\/jats:italic>= 11), aerosol (<jats:italic>n<\/jats:italic>= 6), and exposure to infected deer (in contact,<jats:italic>n<\/jats:italic>= 8), were studied. Upon infection, specific bands of reactivity at \u223c24 to 26 kDa, \u223c33 kDa, \u223c42 kDa, and \u223c75 kDa to<jats:italic>M. bovis<\/jats:italic>whole-cell sonicate were detected by immunoblot. Lipoarabinomannan-specific immunoglobulin was detected as early as 36 days postchallenge, and responses were detected for 94% of intratonsillarly and \u201cin-contact\u201d-infected deer. In MAPIA, sera were tested with 12 native and recombinant antigens coated on nitrocellulose. All in-contact-infected (8 of 8) and 10 of 11 intratonsillarly infected deer produced antibody reactive with one or more of the recombinant\/native antigens. Responses were boosted by injection of tuberculin for intradermal tuberculin skin testing. Additionally, three of six deer receiving a very low dose of<jats:italic>M. bovis<\/jats:italic>via aerosol exposure produced antibody specific to one or more recombinant proteins.<jats:italic>M. bovis<\/jats:italic>was isolated from one of three nonresponding aerosol-challenged deer. Of the 12 antigens tested, the most immunodominant protein was MPB83; however, a highly sensitive serodiagnostic test will likely require use of multiple antigens.<\/jats:p>","DOI":"10.1128\/cdli.11.5.849-855.2004","type":"journal-article","created":{"date-parts":[[2004,9,9]],"date-time":"2004-09-09T16:35:03Z","timestamp":1094747703000},"page":"849-855","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Antigen Recognition by Serum Antibodies in White-Tailed Deer (<i>Odocoileus virginianus<\/i>) Experimentally Infected with<i>Mycobacterium bovis<\/i>"],"prefix":"10.1128","volume":"11","author":[{"given":"W. R.","family":"Waters","sequence":"first","affiliation":[{"name":"National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Bacterial Diseases of Livestock Research Unit, Ames, Iowa"},{"name":"Chembio Diagnostic Systems, Inc., Medford, New York"},{"name":"Bacteriology Department, Veterinary Sciences Division, Stormont, Belfast, United Kingdom"},{"name":"Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. V.","family":"Palmer","sequence":"additional","affiliation":[{"name":"National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Bacterial Diseases of Livestock Research Unit, Ames, Iowa"},{"name":"Chembio Diagnostic Systems, Inc., Medford, New York"},{"name":"Bacteriology Department, Veterinary Sciences Division, Stormont, Belfast, United Kingdom"},{"name":"Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. P.","family":"Bannantine","sequence":"additional","affiliation":[{"name":"National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Bacterial Diseases of Livestock Research Unit, Ames, Iowa"},{"name":"Chembio Diagnostic Systems, Inc., Medford, New York"},{"name":"Bacteriology Department, Veterinary Sciences Division, Stormont, Belfast, United Kingdom"},{"name":"Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. L.","family":"Whipple","sequence":"additional","affiliation":[{"name":"National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Bacterial Diseases of Livestock Research Unit, Ames, Iowa"},{"name":"Chembio Diagnostic Systems, Inc., Medford, New York"},{"name":"Bacteriology Department, Veterinary Sciences Division, Stormont, Belfast, United Kingdom"},{"name":"Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Greenwald","sequence":"additional","affiliation":[{"name":"National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Bacterial Diseases of Livestock Research Unit, Ames, Iowa"},{"name":"Chembio Diagnostic Systems, Inc., Medford, New York"},{"name":"Bacteriology Department, Veterinary Sciences Division, Stormont, Belfast, United Kingdom"},{"name":"Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Esfandiari","sequence":"additional","affiliation":[{"name":"National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Bacterial Diseases of Livestock Research Unit, Ames, Iowa"},{"name":"Chembio Diagnostic Systems, Inc., Medford, New York"},{"name":"Bacteriology Department, Veterinary Sciences Division, Stormont, Belfast, United Kingdom"},{"name":"Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Andersen","sequence":"additional","affiliation":[{"name":"National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Bacterial Diseases of Livestock Research Unit, Ames, Iowa"},{"name":"Chembio Diagnostic Systems, Inc., Medford, New York"},{"name":"Bacteriology Department, Veterinary Sciences Division, Stormont, Belfast, United Kingdom"},{"name":"Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"McNair","sequence":"additional","affiliation":[{"name":"National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Bacterial Diseases of Livestock Research Unit, Ames, Iowa"},{"name":"Chembio Diagnostic Systems, Inc., Medford, New York"},{"name":"Bacteriology Department, Veterinary Sciences Division, Stormont, Belfast, United Kingdom"},{"name":"Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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