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The expression of CD69 on CD4 T lymphocytes was significantly higher in patients with active TB (16.2% \u00b1 7.3%), individuals with clinically inactive TB (10.5% \u00b1 7.4%), and healthy individuals with a positive TST result (15.5% \u00b1 7.2%) than in healthy individuals with a negative TST result (3.8% \u00b1 4.3%) (<jats:italic>P<\/jats:italic>&lt; 0.005). We confirmed the correlation between CD69 antigen expression on T lymphocytes after stimulation with tuberculin and the TST induration diameter (Spearman rho = 0.783;<jats:italic>P<\/jats:italic>&lt; 0.001), an assay for gamma interferon (the Quantiferon-TB assay; Spearman rho = 0.613;<jats:italic>P<\/jats:italic>&lt; 0.001), and the lymphocyte BLAST transformation test (Spearman rho = 0.537;<jats:italic>P<\/jats:italic>&lt; 0.001). Our results demonstrate the usefulness of the determination of CD69 on CD4 T lymphocytes after in vitro stimulation with tuberculin as a rapid indicator of immune sensitization against<jats:italic>Mycobacterium tuberculosis<\/jats:italic>.<\/jats:p>","DOI":"10.1128\/cdli.12.1.101-106.2005","type":"journal-article","created":{"date-parts":[[2005,1,10]],"date-time":"2005-01-10T23:08:03Z","timestamp":1105398483000},"page":"101-106","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["CD69 Expression on CD4<sup>+<\/sup>T Lymphocytes after In Vitro Stimulation with Tuberculin Is an Indicator of Immune Sensitization against<i>Mycobacterium tuberculosis<\/i>Antigens"],"prefix":"10.1128","volume":"12","author":[{"given":"Bojana","family":"Avgustin","sequence":"first","affiliation":[{"name":"Department of Pulmonary Diseases, University Medical Centre, Ljubljana, Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Kotnik","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Immunology, Medical Faculty, University of Ljubljana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mojca","family":"S\u030ckoberne","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Immunology, Medical Faculty, University of Ljubljana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadej","family":"Malovrh","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Immunology, Medical Faculty, University of Ljubljana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleksandra","family":"Skralovnik-Stern","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Diseases, University Medical Centre, Ljubljana, Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marjeta","family":"Terc\u030celj","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Diseases, University Medical Centre, Ljubljana, Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","first-page":"1","volume":"48","year":"1999","unstructured":"Advisory Council on the Elimination of Tuberculosis. 1999. 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