{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T14:03:37Z","timestamp":1790863417485,"version":"4.1.0"},"reference-count":69,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Yersinia enterocolitica<\/jats:italic>\n            O:9 bears a smooth lipopolysaccharide (S-LPS) of\n            <jats:italic>Brucella<\/jats:italic>\n            sp. O-chain A + C\/Y epitopic structure and is a cause of false-positive serological reactions (FPSR) in standard tests for cattle brucellosis.\n            <jats:italic>Brucella<\/jats:italic>\n            S-LPS, cross-reacting S-LPSs representing several O-chain epitope combinations,\n            <jats:italic>Brucella<\/jats:italic>\n            core lipid A epitopes (rough LPS),\n            <jats:italic>Brucella abortus<\/jats:italic>\n            S-LPS-derived polysaccharide, native hapten polysaccharide, rough LPS group 3 outer membrane protein complexes, recombinant BP26, and cytosolic proteins were tested in enzyme-linked immunosorbent assays (ELISA) and precipitation tests to detect cattle brucellosis (sensitivity) and to differentiate it from FPSR (specificity). No single serological test and antigen combination showed 100% sensitivity and specificity simultaneously. Immunoprecipitation tests with native hapten polysaccharide, counterimmunoelectrophoresis with cytosolic proteins, and a chaotropic ELISA with\n            <jats:italic>Brucella<\/jats:italic>\n            S-LPS were 100% specific but less sensitive than the Rose Bengal test, complement fixation, and indirect ELISA with\n            <jats:italic>Brucella<\/jats:italic>\n            S-LPSs and native hapten or S-LPS-derived polysaccharides. A competitive ELISA with\n            <jats:italic>Brucella<\/jats:italic>\n            S-LPS and M84 C\/Y-specific monoclonal antibody was not 100% specific and was less sensitive than other tests. ELISA with\n            <jats:italic>Brucella suis<\/jats:italic>\n            bv. 2 S-LPS (deficient in C epitopes),\n            <jats:italic>Escherichia hermannii<\/jats:italic>\n            S-LPSs [lacking the contiguous \u03b1-(1-2)-linked perosamine residues characteristic of\n            <jats:italic>Y. enterocolitica<\/jats:italic>\n            S-LPS], BP26 recombinant protein, and\n            <jats:italic>Brucella<\/jats:italic>\n            cytosolic fractions did not provide adequate sensitivity\/specificity ratios. Although no serological test and antigen combination fully resolved the diagnosis of bovine brucellosis in the presence of FPSR, some are simple and practical alternatives to the brucellin skin test currently recommended for differential diagnosis.\n          <\/jats:p>","DOI":"10.1128\/cdli.12.1.141-151.2005","type":"journal-article","created":{"date-parts":[[2005,1,10]],"date-time":"2005-01-10T23:08:03Z","timestamp":1105398483000},"page":"141-151","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":73,"title":["Efficacy of Several Serological Tests and Antigens for Diagnosis of Bovine Brucellosis in the Presence of False-Positive Serological Results Due to\n            <i>Yersinia enterocolitica<\/i>\n            O:9"],"prefix":"10.1128","volume":"12","author":[{"given":"P. M.","family":"Mun\u0303oz","sequence":"first","affiliation":[{"name":"Centro de Investigacio\u0301n y Tecnologi\u0301a Agroalimentaria, Gobierno de Arago\u0301n, Zaragoza"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. M.","family":"Mari\u0301n","sequence":"additional","affiliation":[{"name":"Centro de Investigacio\u0301n y Tecnologi\u0301a Agroalimentaria, Gobierno de Arago\u0301n, Zaragoza"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Monreal","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Universidad de Navarra, Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Gonza\u0301lez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Universidad de Navarra, Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Garin-Bastuji","sequence":"additional","affiliation":[{"name":"OIE\/FAO Reference Laboratory for Brucellosis, Agence Franc\u0327aise de Se\u0301curite\u0301 Sanitaire des Aliments, Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Di\u0301az","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Universidad de Navarra, Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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