{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T00:28:11Z","timestamp":1791073691185,"version":"4.1.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2005,2,1]],"date-time":"2005-02-01T00:00:00Z","timestamp":1107216000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2005,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In the United States, there is currently a major gap in the diagnostic capabilities with regard to plague. To address this, we developed an antigen capture assay using an essential virulence factor secreted by\n            <jats:italic>Yersinia<\/jats:italic>\n            spp., LcrV, as the target antigen. We generated anti-LcrV monoclonal antibodies (MAbs) and screened them for the ability to bind bacterially secreted native\n            <jats:italic>Yersinia pestis<\/jats:italic>\n            LcrV. Anti-LcrV MAb 19.31 was used as a capture antibody, and biotinylated MAb 40.1 was used for detection. The detection limit of this highly sensitive\n            <jats:italic>Yersinia<\/jats:italic>\n            LcrV capture enzyme-linked immunosorbent assay is 0.1 ng\/ml. The assay detected LcrV from human sputum and blood samples treated with concentrations as low as 0.5 ng\/ml of bacterially secreted native\n            <jats:italic>Y. pestis<\/jats:italic>\n            LcrV. This assay could be used as a tool to help confirm the diagnosis of plague in patients presenting with pneumonia.\n          <\/jats:p>","DOI":"10.1128\/cdli.12.2.339-346.2005","type":"journal-article","created":{"date-parts":[[2005,2,7]],"date-time":"2005-02-07T22:28:47Z","timestamp":1107815327000},"page":"339-346","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["LcrV Capture Enzyme-Linked Immunosorbent Assay for Detection of\n            <i>Yersinia pestis<\/i>\n            from Human Samples"],"prefix":"10.1128","volume":"12","author":[{"given":"Maria J. C.","family":"Gomes-Solecki","sequence":"first","affiliation":[{"name":"Center for Infectious Diseases"},{"name":"Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook"},{"name":"Brookhaven National Laboratory, Upton, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne G.","family":"Savitt","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases"},{"name":"Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook"},{"name":"Brookhaven National Laboratory, Upton, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca","family":"Rowehl","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases"},{"name":"Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook"},{"name":"Brookhaven National Laboratory, Upton, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John D.","family":"Glass","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases"},{"name":"Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook"},{"name":"Brookhaven National Laboratory, Upton, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James B.","family":"Bliska","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases"},{"name":"Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook"},{"name":"Brookhaven National Laboratory, Upton, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond J.","family":"Dattwyler","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases"},{"name":"Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook"},{"name":"Brookhaven National Laboratory, Upton, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.2.434-464.2004"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.37.2.506-512.1982"},{"key":"e_1_3_2_4_2","unstructured":"Centers for Disease Control and Prevention. 2001. 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