{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T17:56:56Z","timestamp":1788976616114,"version":"build-2803163510"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1995,3,1]],"date-time":"1995-03-01T00:00:00Z","timestamp":794016000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1995,3]]},"abstract":"<jats:p>Juvenile dermatomyositis (JDMS) is a vasculopathy affecting primarily skin and muscle. Although the etiology is unknown, immunopathogenetic mechanisms appear to play a role in both the susceptibility to the disease and its progression. We measured the percentage and absolute numbers of B cells and T-cell subsets in the peripheral blood of untreated JDMS patients with active early disease and compared the results with those obtained from a study of peripheral blood obtained from a heatlhy age-related control group. The absolute number of total lymphocytes in the peripheral blood of the JDMS patients was significantly lower (P &lt; 0.002) than that observed in the healthy control population, with an associated decrease in the absolute number of all T-cell subsets. No concomitant decrease in the absolute number of B lymphocytes was observed in the JDMS patients. In contrast, the percentage of B lymphocytes and the T-helper\/T-suppressor cell ratio were significantly higher in the JDMS group than in the control group (P &lt; 0.001 and P &lt; 0.002, respectively). Retrospective analysis of JDMS patients' serum samples obtained within 1 month of the flow cytometric evaluation indicated that 79% of the sera contained an antinuclear antibody and 46% had immunoglobulin G values above age-adjusted reference ranges. The increased percentage of B cells, the increased T-helper\/T-suppressor cell ratio, the positive antinuclear antibody results, and the increased concentration of serum immunoglobulin suggest that humoral immune dysregulation may contribute to the pathogenesis of JDMS.<\/jats:p>","DOI":"10.1128\/cdli.2.2.205-208.1995","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T18:42:48Z","timestamp":1578336168000},"page":"205-208","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Flow cytometric analyses of the lymphocyte subsets in peripheral blood of children with untreated active juvenile dermatomyositis"],"prefix":"10.1128","volume":"2","author":[{"given":"M R","family":"O'Gorman","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Northwestern University Medical School, Chicago, Illinois 60614."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V","family":"Corrochano","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Northwestern University Medical School, Chicago, Illinois 60614."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Roleck","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Northwestern University Medical School, Chicago, Illinois 60614."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Donovan","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Northwestern University Medical School, Chicago, Illinois 60614."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L M","family":"Pachman","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Northwestern University Medical School, Chicago, Illinois 60614."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Clinical Diagnostic Laboratory Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/cdli.2.2.205-208.1995","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/cdli.2.2.205-208.1995","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T06:02:05Z","timestamp":1645596125000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/cdli.2.2.205-208.1995"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,3]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1995,3]]}},"alternative-id":["10.1128\/cdli.2.2.205-208.1995"],"URL":"https:\/\/doi.org\/10.1128\/cdli.2.2.205-208.1995","relation":{},"ISSN":["1071-412X","1098-6588"],"issn-type":[{"value":"1071-412X","type":"print"},{"value":"1098-6588","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,3]]},"assertion":[{"value":"1995-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}