{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:35:53Z","timestamp":1761060953168},"reference-count":0,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1996,1,1]],"date-time":"1996-01-01T00:00:00Z","timestamp":820454400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1996,1]]},"abstract":"<jats:p>In vitro correlates of type 1 hypersensitivity to natural latex (NL) proteins continue to be limited by both sensitivity and specificity. Methods which have detection limits in the picogram range, namely, radioallergosorbent assays (RAST) and enzyme-linked immunosorbent assays (ELISA), are inadequate for the identification of NL hypersensitivity in certain at-risk groups, such as health care workers. A flow cytometry assay (FCA), previously shown to be comparable to RAST and ELISA in the identification of NL-sensitized pediatric patients with spina bifida, was compared with electrochemiluminescence (ECL) in the evaluation of pediatric patients with spina bifida and NL-sensitized adult health care workers. As with RAST and ELISA, ECL is capable of detecting picogram amounts of specific analyte. The ECL assay detected NL-specific immunoglobulin E (NL-IgE) in three of six health care workers with strong histories of NL hypersensitivity. All six patients were negative by FCA. Further, 2 of 11 spina bifida patients found to be NL-IgE negative by FCA were NL-IgE positive by ECL. These findings suggest that in sensitivity the ECL assay is an improvement over the FCA for the identification of NL-sensitive individuals.<\/jats:p>","DOI":"10.1128\/cdli.3.1.42-46.1996","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T18:36:10Z","timestamp":1578335770000},"page":"42-46","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["A comparison of electrochemiluminescence and flow cytometry for the detection of natural latex-specific human immunoglobulin E"],"prefix":"10.1128","volume":"3","author":[{"given":"L","family":"Kobrynski","sequence":"first","affiliation":[{"name":"Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia 19104, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Tanimune","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia 19104, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N A","family":"Pawlowski","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia 19104, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S D","family":"Douglas","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia 19104, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D E","family":"Campbell","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia 19104, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Clinical Diagnostic Laboratory Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/cdli.3.1.42-46.1996","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/cdli.3.1.42-46.1996","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T06:07:23Z","timestamp":1645596443000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/cdli.3.1.42-46.1996"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1996,1]]}},"alternative-id":["10.1128\/cdli.3.1.42-46.1996"],"URL":"https:\/\/doi.org\/10.1128\/cdli.3.1.42-46.1996","relation":{},"ISSN":["1071-412X","1098-6588"],"issn-type":[{"value":"1071-412X","type":"print"},{"value":"1098-6588","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,1]]},"assertion":[{"value":"1996-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}