{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T13:32:11Z","timestamp":1784640731297,"version":"3.55.0"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[1997,11,1]],"date-time":"1997-11-01T00:00:00Z","timestamp":878342400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1997,11]]},"abstract":"<jats:p>Afipia clevelandensis is a recently described gram-negative bacterium whose potential pathogenic role in human disease is under investigation. Only one strain, from the pretibial lesion of a patient hospitalized with necrotizing pancreatitis for 5 months, has been isolated. Using an indirect immunofluorescence assay to detect anti-A. clevelandensis antibodies, we found a seroprevalence of 1.5% among 30,194 sera routinely submitted for laboratory diagnosis of rickettsial diseases. However, among the 52 patients who were clinically evaluable and who exhibited detectable antibodies against A. clevelandensis, 42% were eventually diagnosed as certainly or probably having brucellosis and 15% were eventually diagnosed as certainly or probably having Yersinia enterocolitica O:9 infection, which is the serotype most often encountered in Europe. Western immunoblotting and cross-adsorption tests showed that an 11.5-kDa proteinase K-labile band and a 21-kDa proteinase-stable band, presumably lipopolysaccharide, were responsible for cross-reactivity among A. clevelandensis, Brucella abortus, and Y. enterocolitica O:9. Other diagnoses included nosocomial infections and various community-acquired diseases for which the role of A. clevelandensis remains undefined. Physicians and clinical microbiologists should be aware of this cross-reactivity in future assessments of the role of A. clevelandensis in human pathology.<\/jats:p>","DOI":"10.1128\/cdli.4.6.748-752.1997","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T18:40:33Z","timestamp":1578336033000},"page":"748-752","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Afipia clevelandensis antibodies and cross-reactivity with Brucella spp. and Yersinia enterocolitica O:9"],"prefix":"10.1128","volume":"4","author":[{"given":"M","family":"Drancourt","sequence":"first","affiliation":[{"name":"Unit\u00e9 des Rickettsies, CNRS UPRESS-A 6020, Facult\u00e9 de M\u00e9decine, Universit\u00e9 de la M\u00e9diterran\u00e9e, Marseille, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Brouqui","sequence":"additional","affiliation":[{"name":"Unit\u00e9 des Rickettsies, CNRS UPRESS-A 6020, Facult\u00e9 de M\u00e9decine, Universit\u00e9 de la M\u00e9diterran\u00e9e, Marseille, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Raoult","sequence":"additional","affiliation":[{"name":"Unit\u00e9 des Rickettsies, CNRS UPRESS-A 6020, Facult\u00e9 de M\u00e9decine, Universit\u00e9 de la M\u00e9diterran\u00e9e, Marseille, France."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Clinical Diagnostic Laboratory Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/cdli.4.6.748-752.1997","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/cdli.4.6.748-752.1997","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T07:27:33Z","timestamp":1645601253000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/cdli.4.6.748-752.1997"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,11]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1997,11]]}},"alternative-id":["10.1128\/cdli.4.6.748-752.1997"],"URL":"https:\/\/doi.org\/10.1128\/cdli.4.6.748-752.1997","relation":{},"ISSN":["1071-412X","1098-6588"],"issn-type":[{"value":"1071-412X","type":"print"},{"value":"1098-6588","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,11]]},"assertion":[{"value":"1997-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}