{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T13:52:49Z","timestamp":1762005169030},"reference-count":28,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1998,3,1]],"date-time":"1998-03-01T00:00:00Z","timestamp":888710400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1998,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>We have previously demonstrated that about one-third of patients with either Sjo\u0308gren\u2019s syndrome (SS) or systemic lupus erythematosus (SLE) react to human immunodeficiency virus (HIV) p24 core protein antigen without any evidence of exposure to, or infection with, HIV itself. Herein, we further characterize the specificity of this reaction using enzyme-linked immunosorbent assay to peptides representing fragments of p24. Characteristic epitope-specific profiles were seen for SS and SLE patients. SS patients had significantly increased responses to peptides F (p24 amino acids 69 to 86) and H (amino acids 101 to 111) and diminished reactivity to peptides A (amino acids 1 to 16) and P (amino acids 214 to 228). SLE patients had increased reactivity to peptides E (amino acids 61 to 76), H, and P. Utilization of peptide P hyporeactivity as the criterion to select for SS patients results in a screen that is moderately sensitive (64%) and specific (79.3%). Adding hyperreactivity to one other peptide (F or H) as an additional criterion yields an expected decrease in sensitivity (to 41%) while increasing specificity (to 93.1%). All sera-reactive peptides from regions of known structure of HIV p24 were located in the apex of the p24 molecule. Thus, the specificity of the peptide reactivities described here indicates a specific pattern of a nonrandom cross-reactivity between HIV type 1 p24 and autoimmune sera which may be partially syndrome specific. The future focus of our work will be to optimize assays of the peptide as diagnostic tools.<\/jats:p>","DOI":"10.1128\/cdli.5.2.181-185.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T19:20:10Z","timestamp":1577820010000},"page":"181-185","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Reactivity of Sera from Systemic Lupus Erythematosus and Sjo\u0308gren\u2019s Syndrome Patients with Peptides Derived from Human Immunodeficiency Virus p24 Capsid Antigen"],"prefix":"10.1128","volume":"5","author":[{"given":"Jane E.","family":"Deas","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology, Immunology and Parasitology1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonita G.","family":"Liu","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology, Immunology and Parasitology1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James J.","family":"Thompson","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology, Immunology and Parasitology1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M.","family":"Sander","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Microbiology and Immunology, Tulane Medical Center,2 New Orleans, Louisiana 70112"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara S.","family":"Soble","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Microbiology and Immunology, Tulane Medical Center,2 New Orleans, Louisiana 70112"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert F.","family":"Garry","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Microbiology and Immunology, Tulane Medical Center,2 New Orleans, Louisiana 70112"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William R.","family":"Gallaher","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology, Immunology and Parasitology1 and"},{"name":"<!--label omitted: 3-->Stanley S. Scott Cancer Center,3 Louisiana State University Medical Center, and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Anderson\nS. C.\nCockayne\nS.\nClinical chemistry: concepts and applications\n1993\n66\n69\nW. B. Saunders Company\nPhiladelphia Pa"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780370109"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1089\/aid.1990.6.1363"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/31.11.735"},{"key":"e_1_3_2_6_2","first-page":"s64","article-title":"A novel retroviral sequence in Sjogren\u2019s syndrome salivary gland biopsy co-cultures.","volume":"35","author":"Brookes S. M.","year":"1992","unstructured":"Brookes S. M. Boyd M. Stephens R. Whitby D. Shattles W. G. Veneables P. J. W. Schultz T. Maini R. N. A novel retroviral sequence in Sjogren\u2019s syndrome salivary gland biopsy co-cultures. Arthritis Rheum. 35 1992 s64","journal-title":"Arthritis Rheum."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780341022"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1089\/aid.1994.10.253"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/milmed\/156.2.93"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1089\/aid.1996.12.491"},{"key":"e_1_3_2_11_2","first-page":"5378","article-title":"Reactivity of serum antibodies from Sjogren\u2019s syndrome and lupus erythematosus patients with HIV capsid peptides.","volume":"38","author":"Gallaher W. R.","year":"1995","unstructured":"Gallaher W. R. Deas J. E. Luo-Zhang H. Sander D. Garry R. F. Reactivity of serum antibodies from Sjogren\u2019s syndrome and lupus erythematosus patients with HIV capsid peptides. Arthritis Rheum. 38 1995 5378","journal-title":"Arthritis Rheum."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1089\/aid.1990.6.1361"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780370405"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1701273"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(89)91027-1"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.273.5272.231"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.4.2866-2872.1997"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1089\/aid.1996.12.1367"},{"key":"e_1_3_2_19_2","first-page":"1","article-title":"Retroviruses and autoimmune rheumatic diseases.","volume":"98","author":"Kalden J. R.","year":"1994","unstructured":"Kalden J. R. Gay S. Retroviruses and autoimmune rheumatic diseases. Rev. Clin. Exp. Immunol. 98 1994 1 5","journal-title":"Rev. Clin. Exp. Immunol."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780351133"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/162.6.1379"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780351212"},{"key":"e_1_3_2_23_2","unstructured":"Sander D. M. Unpublished data."},{"key":"e_1_3_2_24_2","unstructured":"Sell\nS.\nAutoimmune diseases II\u2014diffuse connective tissue (collagen-vascular rheumatoid) diseases\nImmunology immunopathology & immunity.\nSell\nS.\nBerkower\nI.\nMax\nE. E.\n1996\n551\n587\nAppleton & Lange\nStamford Conn"},{"key":"e_1_3_2_25_2","unstructured":"Siegel\nA. F.\nMorgan\nC. J.\nStatistics and data analysis\n2nd ed.\n1996\n341\n343\nJohn Wiley & Sons Inc.\nNew York N.Y"},{"key":"e_1_3_2_26_2","unstructured":"Talal\nN.\nSjogren\u2019s syndrome and connective tissue disease with other immunologic disorders\nArthritis and allied conditions.\nHollander\nJ. L.\n1972\n849\n865\nLea & Febiger\nPhiladelphia Pa"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780330603"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114647"},{"key":"e_1_3_2_29_2","first-page":"2456","article-title":"Detection of retrovirus in salivary glands of patients with Sjogren\u2019s syndrome.","volume":"53","author":"Yamano S.","year":"1995","unstructured":"Yamano S. Saito J. Detection of retrovirus in salivary glands of patients with Sjogren\u2019s syndrome. Nippon Rensho 53 1995 2456 2460","journal-title":"Nippon Rensho"}],"container-title":["Clinical Diagnostic Laboratory Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.5.2.181-185.1998","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.5.2.181-185.1998","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T03:19:39Z","timestamp":1645586379000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CDLI.5.2.181-185.1998"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,3]]},"references-count":28,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1998,3]]}},"alternative-id":["10.1128\/CDLI.5.2.181-185.1998"],"URL":"https:\/\/doi.org\/10.1128\/cdli.5.2.181-185.1998","relation":{},"ISSN":["1071-412X","1098-6588"],"issn-type":[{"value":"1071-412X","type":"print"},{"value":"1098-6588","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,3]]},"assertion":[{"value":"1997-06-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1997-12-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}