{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T19:41:51Z","timestamp":1767987711735,"version":"3.49.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1998,3,1]],"date-time":"1998-03-01T00:00:00Z","timestamp":888710400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1998,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The O157 antigen of<jats:italic>Escherichia coli<\/jats:italic>shares structural elements with lipopolysaccharide (LPS) antigens of other bacterial species, notably<jats:italic>Brucella abortus<\/jats:italic>and<jats:italic>Yersinia enterocolitica<\/jats:italic>O9, a fact that confounds the interpretation of assays for anti-O157 antibodies. To address this problem, a blocking enzyme-linked immunosorbent assay (bELISA) was designed with<jats:italic>E. coli<\/jats:italic>O157:H7 LPS as the antigen and a monoclonal antibody specific for<jats:italic>E. coli<\/jats:italic>O157, designated 13B3, as the competing antibody. The bELISA had equivalent sensitivity to, and significantly higher specificity than, the indirect ELISA (iELISA), detecting anti-O157 antibodies in sera from cattle experimentally inoculated with O157:H7. Only 13% of sera from naive heifers vaccinated for or experimentally infected with<jats:italic>B. abortus<\/jats:italic>had increased anti-O157 bELISA titers, while 61% of anti-O157 iELISA titers were increased. The bELISA is a sensitive and specific method for the detection of serum antibodies resulting from exposure to<jats:italic>E. coli<\/jats:italic>O157.<\/jats:p>","DOI":"10.1128\/cdli.5.2.242-246.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T19:20:24Z","timestamp":1577820024000},"page":"242-246","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Development of a Blocking Enzyme-Linked Immunosorbent Assay for Detection of Serum Antibodies to O157 Antigen of<i>Escherichia coli<\/i>"],"prefix":"10.1128","volume":"5","author":[{"given":"William","family":"Laegreid","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->U.S. Meat Animal Research Center, USDA Agricultural Research Service, Clay Center, Nebraska,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Hoffman","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->National Animal Disease Center, USDA Agricultural Research Service, Ames, Iowa2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Keen","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->U.S. Meat Animal Research Center, USDA Agricultural Research Service, Clay Center, Nebraska,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Elder","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->U.S. Meat Animal Research Center, USDA Agricultural Research Service, Clay Center, Nebraska,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jimmy","family":"Kwang","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->U.S. Meat Animal Research Center, USDA Agricultural Research Service, Clay Center, Nebraska,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.epirev.a017914"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02092278"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/175.3.726"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(05)82049-9"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.25.8.1486-1489.1987"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1984.tb07994.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.42.9.973"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)90801-U"},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.2460\/ajvr.1996.57.08.1153","article-title":"Effects of age at vaccination on efficacy of Brucella abortus strain RB51 to protect cattle against brucellosis.","volume":"57","author":"Cheville N. F.","year":"1996","unstructured":"Cheville N. F. Olsen S. C. Jensen A. E. Stevens M. G. Palmer M. V. Florance A. M. Effects of age at vaccination on efficacy of Brucella abortus strain RB51 to protect cattle against brucellosis. Am. J. Vet. Res. 57 1996 1153 1156","journal-title":"Am. J. Vet. Res."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.4.1586-1590.1995"},{"key":"e_1_3_2_12_2","first-page":"2679","article-title":"Electrophoretic and immunochemical study of the lipopolysaccharides produced by chemostat-grown Escherichia coli O157.","volume":"133","author":"Dodds K. L.","year":"1987","unstructured":"Dodds K. L. Perry M. B. McDonald I. J. Electrophoretic and immunochemical study of the lipopolysaccharides produced by chemostat-grown Escherichia coli O157. J. Gen. Microbiol. 133 1987 2679 2687","journal-title":"J. Gen. Microbiol."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01717448"},{"key":"e_1_3_2_14_2","unstructured":"Ferris K. E. Miller D. A. Salmonella serotypes from animals and related sources reported during July 1995-June 1996 Proceedings of the 100th Annual Meeting of the United States Animal Health Association 1996 505 526"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"46","DOI":"10.2460\/javma.1995.207.01.46","article-title":"Risk factors for fecal shedding of Escherichia coli O157:H7 in dairy calves.","volume":"207","author":"Garber L. P.","year":"1995","unstructured":"Garber L. P. Wells S. J. Hancock D. D. Doyle M. P. Tuttle J. Shere J. A. Zhao T. Risk factors for fecal shedding of Escherichia coli O157:H7 in dairy calves. J. Am. Vet. Med. Assoc. 207 1995 46 49","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(95)00121-P"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268800051633"},{"key":"e_1_3_2_18_2","unstructured":"Hoffman M. T. Casey W. Laegreid and B. Bosworth. Bovine immune response to Escherichia coli O157:H7. Submitted for publication."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.135.16.382"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.5.1879-1883.1996"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)93151-0"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.5.1391-1395.1990"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6215(00)90103-4"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1139\/o86-004"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.96.3.600"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.9.2053-2057.1996"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198303243081203"},{"key":"e_1_3_2_28_2","unstructured":"Rosner B. Fundamental biostatistics 1987 369 441 Duxbury Press Boston Mass"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0895-4356(91)90128-V"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.110.9.202-a"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/159.2.344"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/93.2.252"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.3.679-684.1997"},{"key":"e_1_3_2_34_2","doi-asserted-by":"crossref","first-page":"1168","DOI":"10.2460\/javma.1994.204.08.1168","article-title":"Animals as a source of Escherichia coli pathogenic for human beings.","volume":"204","author":"Whipp S. C.","year":"1994","unstructured":"Whipp S. C. Rasmussen M. A. Cray W. C. Animals as a source of Escherichia coli pathogenic for human beings. J. Am. Vet. Med. Assoc. 204 1994 1168 1175","journal-title":"J. Am. Vet. Med. Assoc."}],"container-title":["Clinical Diagnostic Laboratory Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.5.2.242-246.1998","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.5.2.242-246.1998","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,29]],"date-time":"2024-07-29T13:37:50Z","timestamp":1722260270000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CDLI.5.2.242-246.1998"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,3]]},"references-count":33,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1998,3]]}},"alternative-id":["10.1128\/CDLI.5.2.242-246.1998"],"URL":"https:\/\/doi.org\/10.1128\/cdli.5.2.242-246.1998","relation":{},"ISSN":["1071-412X","1098-6588"],"issn-type":[{"value":"1071-412X","type":"print"},{"value":"1098-6588","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,3]]},"assertion":[{"value":"1997-09-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1997-11-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}