{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T14:36:24Z","timestamp":1790001384067,"version":"4.0.1"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[1998,7,1]],"date-time":"1998-07-01T00:00:00Z","timestamp":899251200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1998,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>In 40 cases of human paragonimiases caused by<jats:italic>Paragonimus westermani<\/jats:italic>(20 cases),<jats:italic>P. miyazakii<\/jats:italic>(10 cases), and<jats:italic>P. skrjabini<\/jats:italic>(10 cases), responses of serum immunoglobulin G (IgG), IgG subclasses, and IgE were analyzed by immunoblotting with crude antigens prepared from egg, 4-week-old juvenile, and adult forms of<jats:italic>P. westermani<\/jats:italic>. The 32- and 35-kDa proteins in the adult extracts showed specific reactions regardless of the causative species (39 of 40 cases; 98%). Sera of patients infected with<jats:italic>P. westermani<\/jats:italic>and<jats:italic>P. miyazakii<\/jats:italic>reacted strongly with the 28-, 46-, and 94-kDa proteins of egg extracts, while those from patients infected with<jats:italic>P. skrjabini<\/jats:italic>reacted faintly. No sera from patients with other trematodiases (0 of 15 cases), cestodiases (0 of 20 cases), or lung cancer (0 of 5 cases) or from healthy controls (0 of 10 individuals) showed positive reactions. Analysis by IgG subclass revealed that IgG4 (33 of 40 cases; 83%) and IgG1 (29 of 40 cases; 73%) antibodies in the patient sera recognized the 32- and 35-kDa proteins predominantly. IgG3 reaction was found in 50% (10 of 20 cases) and 30% (3 of 10 cases) of the sera of patients infected with<jats:italic>P. westermani<\/jats:italic>and<jats:italic>P. miyazakii<\/jats:italic>, respectively. In an IgE immunoblot, 83% (33 of 40 cases) of the sera from paragonimiasis patients reacted with the 32- and 35-kDa proteins while no sera from patients with heterologous diseases and healthy controls showed a positive reaction. Both 32- and 35-kDa proteins in adult extracts of<jats:italic>P. westermani<\/jats:italic>were highly reliable for serodiagnosis of human paragonimiases.<\/jats:p>","DOI":"10.1128\/cdli.5.4.474-478.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T19:20:52Z","timestamp":1577820052000},"page":"474-478","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Immunoglobulin G (IgG) Subclass and IgE Responses in Human Paragonimiases Caused by Three Different Species"],"prefix":"10.1128","volume":"5","author":[{"given":"Yoon","family":"Kong","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Molecular Parasitology, College of Medicine, Sung Kyun Kwan University, Suwon 440-746,1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Ito","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Parasitology, Gifu University School of Medicine, Gifu 500,2 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun-Jong","family":"Yang","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Biomedical Research Center, Korea Institute of Science and Technology, Seoul 136-7093 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young-Bae","family":"Chung","sequence":"additional","affiliation":[{"name":"<!--label omitted: 4-->Department of Parasitology, Catholic University of Korea, School of Medicine,4 Seoul 137-701, Korea;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiro","family":"Kasuya","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Parasitology, Gifu University School of Medicine, Gifu 500,2 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masashi","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"<!--label omitted: 5-->Department of Parasitology, School of Medicine, Chiba University, Chiba 260,5 Japan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue-Han","family":"Liu","sequence":"additional","affiliation":[{"name":"<!--label omitted: 6-->Institute of Infectious and Parasitic Diseases, Chongqing University of Medical Sciences, Chongqing, China6"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung-Yull","family":"Cho","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Molecular Parasitology, College of Medicine, Sung Kyun Kwan University, Suwon 440-746,1"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1159\/000236582"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.2214\/ajr.162.1.8273653"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.1989.27.1.15"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.1989.27.1.15"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.2307\/3284286"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1994.tb03513.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1996.55.434"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1148\/radiographics.13.3.8316665"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(87)90267-7"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(91)90096-P"},{"key":"e_1_3_2_12_2","first-page":"347","article-title":"An antigenic component for the serodiagnosis of paragonimiasis miyazakii.","volume":"37","author":"Itoh M.","year":"1988","unstructured":"Itoh M. 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