{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,24]],"date-time":"2024-08-24T10:59:40Z","timestamp":1724497180996},"reference-count":10,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[1998,9,1]],"date-time":"1998-09-01T00:00:00Z","timestamp":904608000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1998,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>The potential roles of specific antibodies of the different immunoglobulin G (IgG) subclasses in the serological diagnosis of cystic echinococcosis (CE) and alveolar echinococcosis (AE) were investigated by an enzyme-linked immunosorbent assay based on hydatid fluid as antigen. Specific antibodies of subclass 1 were found to be of major importance. In sera collected at the time of diagnosis (i.e., before any therapeutic intervention was initiated) they could be demonstrated in 14 of 15 sera from patients with CE and in all 12 sera from patients with AE. The most discriminatory and the most specific antibodies found in this study belonged to IgG subclass 4. Only one false-positive reaction was observed with 253 sera from healthy volunteers, and no cross-reactions occurred in 80 sera from patients with different parasitic infections. Specific IgG4 antibodies could be demonstrated in 61.0 to 66.7% (CE) or 47.6 to 66.7% (AE) of the cases. Antibody levels of IgG subclass 2 were elevated only moderately, and subclass 3 antibodies were detected in a few cases only. In addition, nonspecific reactions in sera of healthy volunteers or patients with other parasitic infections could partially be attributed to antibodies of subclasses 2 and 3.<\/jats:p>","DOI":"10.1128\/cdli.5.5.613-616.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T19:21:10Z","timestamp":1577820070000},"page":"613-616","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Analysis of Specific Immunoglobulin G Subclass Antibodies for Serological Diagnosis of Echinococcosis by a Standard Enzyme-Linked Immunosorbent Assay"],"prefix":"10.1128","volume":"5","author":[{"given":"Felix","family":"Grimm","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Institute of Parasitology, University of Zurich, Zu\u0308rich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Friedrich E.","family":"Maly","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institute of Parasitology, University of Zurich, Zu\u0308rich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Lu\u0308","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institute of Parasitology, University of Zurich, Zu\u0308rich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Llano","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institute of Parasitology, University of Zurich, Zu\u0308rich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1159\/000236582"},{"key":"e_1_3_2_3_2","unstructured":"Craig\nP. S.\nImmunodiagnosis of Echinococcus granulosus\nCompendium on cystic echinococcosis.\nAnderson\nF. L.\n1993\n85\n118\nBrigham Young University\nProvo Utah"},{"key":"e_1_3_2_4_2","first-page":"347","article-title":"Serological differentiation between Echinococcus granulosus and E. multilocularis infection in man.","volume":"69","author":"Gottstein B.","year":"1983","unstructured":"Gottstein B. Eckert J. Frey H. Serological differentiation between Echinococcus granulosus and E. multilocularis infection in man. Parasitol. Res. 69 1983 347 356","journal-title":"Parasitol. Res."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.5.3.248"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0888-0786(90)90016-H"},{"key":"e_1_3_2_7_2","unstructured":"Llano\nR.\nM.D. thesis.\n1997\nUniversity of Zurich\nZurich Switzerland"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1995.tb03778.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-706X(96)00637-7"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1994.51.741"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(95)90449-2"}],"container-title":["Clinical Diagnostic Laboratory Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.5.5.613-616.1998","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.5.5.613-616.1998","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T03:25:06Z","timestamp":1645586706000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CDLI.5.5.613-616.1998"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,9]]},"references-count":10,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1998,9]]}},"alternative-id":["10.1128\/CDLI.5.5.613-616.1998"],"URL":"https:\/\/doi.org\/10.1128\/cdli.5.5.613-616.1998","relation":{},"ISSN":["1071-412X","1098-6588"],"issn-type":[{"value":"1071-412X","type":"print"},{"value":"1098-6588","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,9]]},"assertion":[{"value":"1997-09-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-06-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}