{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:19:56Z","timestamp":1790875196586,"version":"4.1.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[1998,9,1]],"date-time":"1998-09-01T00:00:00Z","timestamp":904608000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1998,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The<jats:italic>Toxoplasma gondii<\/jats:italic>rhoptry protein Rop2 was expressed in<jats:italic>Escherichia coli<\/jats:italic>as a fusion protein containing 44 kDa of the 55-kDa mature Rop2, supplied with six histidyl residues at the N-terminal end (Rop2<jats:sub>196\u2013561<\/jats:sub>). Humoral response during<jats:italic>Toxoplasma<\/jats:italic>infection of humans was analyzed by immunoglobulin G (IgG), IgA, and IgM enzyme-linked immunosorbent assay with Rop2<jats:sub>196\u2013561<\/jats:sub>as the antigen substrate. The analyzed sera were divided according to<jats:italic>T. gondii<\/jats:italic>-specific serological tests (IgG, IgA, or IgM indirect immunofluorescence and IgA or IgM immunosorbent agglutination assay) as group A (IgG<jats:sup>+<\/jats:sup>IgA<jats:sup>\u2212<\/jats:sup>IgM<jats:sup>\u2212<\/jats:sup>;<jats:italic>n<\/jats:italic>= 35), group B (IgG<jats:sup>+<\/jats:sup>IgA<jats:sup>+<\/jats:sup>IgM<jats:sup>+<\/jats:sup>;<jats:italic>n<\/jats:italic>= 21), group C (IgG<jats:sup>+<\/jats:sup>IgA<jats:sup>+<\/jats:sup>IgM<jats:sup>\u2212<\/jats:sup>;<jats:italic>n<\/jats:italic>= 5), and group D (IgG<jats:sup>+<\/jats:sup>IgA<jats:sup>\u2212<\/jats:sup>IgM<jats:sup>+<\/jats:sup>;<jats:italic>n<\/jats:italic>= 16). Twenty-six<jats:italic>T. gondii<\/jats:italic>-seronegative sera from individuals with other infections were also included (group E). Anti-Rop2 IgG antibodies were detected in 82.8% of group A sera and in 97.6% of the sera with acute-phase marker immunoglobulins (groups B, C, and D). The percentage of IgA antibody reactivity against Rop2<jats:sub>196\u2013561<\/jats:sub>was 17.1% in group A, 50% in group D, and 80.8% in groups B and C. The percentage of IgM antibody reactivity was 0% in groups A and C and 62% in groups B and D. Sera from group E failed to show IgA, IgM, or IgG antibody reactivity. Since<jats:italic>T. gondii<\/jats:italic>Rop2 elicits a strong humoral response from an early stage of infection, it is suggested that recombinant Rop2<jats:sub>196\u2013561<\/jats:sub>would be suitable for use in diagnostic systems, in combination with other<jats:italic>T. gondii<\/jats:italic>antigens, to detect specific IgG, IgA, and IgM antibodies.<\/jats:p>","DOI":"10.1128\/cdli.5.5.627-631.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T19:21:20Z","timestamp":1577820080000},"page":"627-631","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Detection of Human<i>Toxoplasma<\/i>-Specific Immunoglobulins A, M, and G with a Recombinant<i>Toxoplasma gondii<\/i>Rop2 Protein"],"prefix":"10.1128","volume":"5","author":[{"given":"Valentina","family":"Martin","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Parasitolog\u0131\u0301a, ANLIS Dr. Carlos G. 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