{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T14:02:27Z","timestamp":1790863347979,"version":"4.1.0"},"reference-count":13,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[1998,9,1]],"date-time":"1998-09-01T00:00:00Z","timestamp":904608000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Diagn Lab Immunol"],"published-print":{"date-parts":[[1998,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The results of a field trial conducted in Latin America with two indirect enzyme-linked immunosorbent assays (ELISAs) and two competitive ELISAs (CELISAs) for the detection of bovine antibody to\n            <jats:italic>Brucella abortus<\/jats:italic>\n            are reported. One of the CELISA formats performed most accurately. The percentage of positive reactions in the CELISA relative to the selected positive rose bengal agglutination test (RBT) and complement fixation test (CFT) results was 97.47%, the percentage of negatives relative to the selected negative RBT and CFT results for unexposed cattle was 98.32%, and the percentage of negatives in cattle vaccinated with\n            <jats:italic>B. abortus<\/jats:italic>\n            19 was 96.51%. The same assay format under Canadian conditions had an actual sensitivity of 100%, a specificity of 99.90% in nonvaccinates, and a specificity of 97.7% in a strain 19-vaccinated population. Overall, the CELISA performed as expected and the results were not dissimilar from the results obtained in the Canadian study. This provided further evidence that this CELISA can in many instances differentiate infected cattle from those that are vaccinated or infected with a cross-reacting organism while still giving very few false-positive or false-negative results.\n          <\/jats:p>","DOI":"10.1128\/cdli.5.5.654-661.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T19:21:23Z","timestamp":1577820083000},"page":"654-661","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Enzyme Immunoassays for Serological Diagnosis of Bovine Brucellosis: A Trial in Latin America"],"prefix":"10.1128","volume":"5","author":[{"given":"D.","family":"Gall","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Canadian Food Inspection Agency, Animal Diseases Research Institute, Nepean, Ontario, Canada K2H 8P91;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Colling","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->International Atomic Energy Agency, A1400, Vienna, Austria2;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Marino","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Instituto Colombiano Agropecuaria, ICA-CEISA, Santafe de Bogota DC, Colombia3;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Moreno","sequence":"additional","affiliation":[{"name":"<!--label omitted: 4-->Tropical Diseases Research Program, School of Veterinary Medicine, National University, Heredia, Costa Rica4;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Nielsen","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Canadian Food Inspection Agency, Animal Diseases Research Institute, Nepean, Ontario, Canada K2H 8P91;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Perez","sequence":"additional","affiliation":[{"name":"<!--label omitted: 5-->Servicios Agricolas y Ganaderos, Laboratorio Regional Osorno, Mackenna 674, Osorno, Chile5; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Samartino","sequence":"additional","affiliation":[{"name":"<!--label omitted: 6-->Instituto de Patobiologia-DPTO Bacteriologia, INTA-CICV, CC 77, 1708 Moron, Buenos Aires, Argentina6"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"168","article-title":"Enzyme linked immunosorbent assay (ELISA) for titration of antibodies against Brucella abortus and Yersinia enterocolitica.","volume":"84","author":"Carlsson H. E.","year":"1976","unstructured":"Carlsson H. E. Hurvell B. Lindberg A. A. Enzyme linked immunosorbent assay (ELISA) for titration of antibodies against Brucella abortus and Yersinia enterocolitica. Acta Pathol. Microbiol. Scand. 84 1976 168 176","journal-title":"Acta Pathol. Microbiol. Scand."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/15321819408009578"},{"key":"e_1_3_2_4_2","unstructured":"MacMillian\nA.\nConventional serological tests\nAnimal brucellosis.\nNielsen\nK.\nDuncan\nJ. R.\n1990\n153\nCRC Press Inc.\nBoca Raton Fla"},{"key":"e_1_3_2_5_2","first-page":"83","article-title":"A competitive enzyme immunoassay for brucellosis diagnosis.","volume":"97","author":"MacMillian A.","year":"1990","unstructured":"MacMillian A. Greiser-Wilke I. Moennig V. Mathias L. A competitive enzyme immunoassay for brucellosis diagnosis. Dtsch. Tieraerztl. Wochenschr. 97 1990 83","journal-title":"Dtsch. Tieraerztl. Wochenschr."},{"key":"e_1_3_2_6_2","first-page":"5","article-title":"Enzyme immunoassay for the differentiation of the antibody response of Brucella abortus infected or strain 19 vaccinated cattle.","volume":"50","author":"Nielsen K.","year":"1989","unstructured":"Nielsen K. Cherwonogrodzky J. Duncan J. R. Bundle D. R. Enzyme immunoassay for the differentiation of the antibody response of Brucella abortus infected or strain 19 vaccinated cattle. Am. J. Vet. Res. 50 1989 5","journal-title":"Am. J. Vet. Res."},{"key":"e_1_3_2_7_2","unstructured":"Nielsen\nK.\nGall\nD.\nKelly\nW.\nVigliocco\nA.\nHenning\nD.\nGarcia\nM.\nImmunoassay development: application to enzyme immunoassay for diagnosis of brucellosis. Monograph.\n1996\nAgriculture and Agri-Food Canada Animal Diseases Research Institute\nNepean Ontario Canada"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2427(88)90160-2"},{"key":"e_1_3_2_9_2","unstructured":"Nielsen\nK. H.\nGall\nD.\nKelly\nW.\nHenning\nD.\nGarcia\nM. M.\nEnzyme immunoassay: application to diagnosis of bovine brucellosis. Monograph.\n1992\nAgriculture Canada\nNepean Ontario Canada"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5877(95)00513-7"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-2607(95)01703-8"},{"key":"e_1_3_2_12_2","unstructured":"U.S. Department of Agriculture\nNADL diagnostic reagents manual\n1965\n65C\n65E\nDiagnostic Reagents Division National Animal Diseases Laboratory U.S. Department of Agriculture\nAmes Iowa"},{"key":"e_1_3_2_13_2","unstructured":"U.S. Department of Health Education and Welfare\nStandardized diagnostic complement fixation and adaptation to the microtest. Public health monograph N74. U.S.\n1965\nDepartment of Health Education and Welfare\nWashington D.C"},{"key":"e_1_3_2_14_2","unstructured":"Wright\nP. F.\nNielsen\nK.\nKelly\nW.\nPrimary binding assay techniques for the serodiagnosis of bovine brucellosis\u2014enzyme immunoassay\nAnimal brucellosis.\nNielsen\nK.\nDuncan\nJ. R.\n1990\n199\nCRC Press Inc.\nBoca Raton Fla"}],"container-title":["Clinical Diagnostic Laboratory Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.5.5.654-661.1998","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CDLI.5.5.654-661.1998","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T03:25:35Z","timestamp":1645586735000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CDLI.5.5.654-661.1998"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,9]]},"references-count":13,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1998,9]]}},"alternative-id":["10.1128\/CDLI.5.5.654-661.1998"],"URL":"https:\/\/doi.org\/10.1128\/cdli.5.5.654-661.1998","relation":{},"ISSN":["1071-412X","1098-6588"],"issn-type":[{"value":"1071-412X","type":"print"},{"value":"1098-6588","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,9]]},"assertion":[{"value":"1998-02-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-05-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}